Biomedical subjects

S R Luo

Publications and source records attributed to S R Luo.

13 recordsLinked to original sources

PubMed1993

[Study on lignans from Diphylleia sinensis].

A new lignan along with eight known lignans and a flavonoid were isolated from the rhizomes of Diphylleia sinensis Li.. Their structures were elucidated on the basis of chemical and spectral analysis. The new lignan, compound IX, was named as picropodophyllin-1-ethyl ether (IX). The eight known lignans were identified as podophyllotoxin (I), isopicropodophyllone (II), dehydropodophyllotoxin (III), diphyllin (IV), picropodophyllin (V), podophyllotoxone (VI), 4'-demethylpodophyllotoxin (VII) and picropodophyllin glucoside (VIII). Compounds II, VI, VII and VIII were found for the first time from the rhizome of this plant. The flavonoid was identified as kaempferol.

Antineoplastic Agents, Phytogenic↗

PubMed1993

[Determination of gelsemium alkaloids by RP-HPLC].

Gelsemium elegans Benth is a kind of traditional Chinese medicine, eight alkaloids have been isolated from this herb. In recent years, an HPLC method for the separation and determination of five of these alkaloids, ie. gelsemium A (G), koumine (F), kumantenidine (D), kumantenine (B) and kumantenmine (A) is described, dichroine being used as the internal standard. In this report a RP column of C18 and the mobile phase methanol--water--n-butylamine (78:22:0.1 V/V) were employed. The flow rate was 1.0 ml/min, the column temperature was 25 degrees C and the detection wavelength was 256 nm. The calibration curves showed good linearity over the range of 0.02-0.12 microgram, r = 0.9835-0.9977 and the recoveries were 95.01-99.70% for the five alkaloids. The method is simple, sensitive and reproducible and can be used for the quality control of Gelsemium preparations for clinical evaluation.

Alkaloids↗

PubMed1992

[Studies on the TLC scanning determination of lignans in Diphylleia sinensis Li].

A simple, sensitive and accurate method for the separation and determination of the lignans: podophylltoxone (I), isopicropodophyllone (II), picropodophyllone (III), dehydropodophyllotoxin (IV), picropodophyllin (V), podophyllotoxin (VI), 4'-demethylpodophyllotoxin (VII) and diphyllin (VIII) is described. The sample solution was applied at a point 1 cm from the bottom edge of the HPTLC silica gel plate (10 cm x 10 cm), dichloromethane-diethyl ether (4:1) was used as the developing solvent. The plate was saturated for 30 min and then developed twice for 9.5 cm using ascending technique. The plate was sprayed with 2.5% ammonium ceric sulphate--20% nitric acid and toasted for 15 min at 120 degrees C, then fumigated with ammonia solution for 20 min at room temperature to intensify the spot color. The spots were scanned with a Shimadzu CS-930 TLC scanner. The contents of eight lignans in Diphylleia sinensis was calculated by comparison with standards spotted on the same plate. The standard curves were linear in the range of 0.48-2.52 micrograms for the eight lignans. The method has been applied to the analysis of various samples and can be used for the quality control of Diphylleia sinensis, podophyllum and dysosma preparations used in clinic.

Chromatography, Thin Layer↗

PubMed1991

[Comparative study of the relationship between in vitro motility and invasion potentials of three mouse forestomach cancer (MFC) cell lines].

The relationship between motility of cancer cells and their invasiveness is important in understanding the invasion mechanisms of malignant tumors. The in vitro motility of three MFC cell lines was measured by the Boyden chamber technique. Statistical analysis of the results showed significant differences in vitro motility among these three cell lines. The motility correlated with in vitro invasion potentials.

Animals↗

PubMed1991

[Determination of naphthoquinones in tissue cultured Zicao].

A simple, sensitive and accurate method for the separation and determination of the naphthoquinones, deoxyshikonin (I), acetylshikonin (II) and beta-beta-dimethylacrylshikonin (III) in tissue cultured Zicao is described. The sample solution was applied at a point 1 cm from the bottom edge of the TLC silica gel plate (10 x 15 cm). A mixture of n-hexane-acetone-chloroform-glacial acetic acid (13:0.3:0.2:0.05 v/v) was used as the developing solvent. The plate was saturated for 30 min and then developed twice for 15 cm using ascending technique. The spots were scraped off and extracted with CHCl3, then determined spectrophotometrically. The standard curve was linear in the range of 0.65-2.25 micrograms for the naphthoquinones. The method has been applied to the analysis of different samples. This method can be used for the quality control of naphthoquinones in tissue culture products.

Chromatography, Thin Layer↗

PubMed1989

Establishment, characteristics, and utilization of a new in vivo-in vitro system.

Radiotherapy and chemotherapy are two important methods for malignant tumor treatment. To research radiobiological response in therapy, we have established a better experimental method in contrast to the traditional ones such as TCD50, regrowth delay, cell survival curve, etc, all with their limitations. A new mouse tumor in vivo-in vitro system LA795 Vv-Vt has been developed for studies on radiobiology. Such a system could be used to study the in vivo response of a solid tumor by the in vitro cloning assay. For the purpose of increasing the PE in vitro, LA795 Vv-Vt tumor line was purified through culturing the cells as a clonogenic spheroid. The spheroids were then injected into the flank of mouse subcutaneously for tumor growth. The in vivo-in vitro system LA795 Vv-Vt is an excellent model dissecting and analyzing the various factors which affect tumor development and determine the response of tumor to specific agent and regimens.

Animals↗

PubMed1988