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S RAYMOND

Publications and source records attributed to S RAYMOND.

At least 19 recordsLinked to original sources
PubMed1964

PROTEIN PURIFICATION BY ELUTION CONVECTION ELECTROPHORESIS.

Purified protein components can be recovered from high-resolution gel-electrophoresis patterns by elution-convection electrophoresis. The protein components are all simultaneously eluted from the gel pattern by horizontal electrophoresis and are then simultaneously concentrated by electro-convection to recover the proteins in nearly the original sample concentration. Thirty individual protein fractions can be recovered in 3 hours from a sample containing 200 milligrams of protein.

Convection↗
PubMed1963

ANTISERUMS PREPARED WITH ACRYLAMIDE GEL USED AS ADJUVANT.

Antiserums to macroglobulins were prepared by a convenient and time-saving procedure in which acrylamide gel was used as adjuvant. After electrophoresis in acrylamide gel, the macroglobulin zone from the gel pattern was homogenized and injected directly into rabbits without intermediate isolation of the purified antigen. The resulting antiserums exhibited high titers of antiserum to macroglobulin and showed only weak cross-reactions with other gamma globulins in serologic tests.

Acrylamide↗
PubMed1959

Novobiocin jaundice.

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Anti-Bacterial Agents↗
PubMed1952

The isolation and identification of para-aminobenzoic acid produced by staphylococci resistant to sulfonamide.

Previous studies have shown that when Staphylococcus aureus becomes resistant to sulfonamides, an antisulfonamide substance is produced in the growth medium. Although these studies suggested that the substance was p-aminobenzoic acid (PABA), isolation and positive identification were not achieved. Because of the importance of these observations, unequivocal identification of the product was attempted. A strain of Stapkylococcus aureus resistant to sulfonamides was cultivated on a simplified medium, the organisms separated in a Sharples centrifuge and the diazotisable amine absorbed on amberlite IR-120 previously acidified. Elution was accomplished with pyridine and the amine recovered by ether extraction at pH 3.7. Paper chromatography revealed one amine resembling PABA and another contaminating diazotizable amine present in very small amounts. The PABA-like amine was further separated by chromatography; its R(f) value and its spectrum in the ultraviolet then equalled those obtained with PABA. A 2,4-dlnltrophenyl derivative of the amine was prepared and the m.p. was similar to that of the derivative made from PABA. On the basis of the physical and chemical properties described, it would appear unequivocal that this bacterial amine is PABA.

4-Aminobenzoic Acid↗