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Biomedical subjects

S Rauf

Publications and source records attributed to S Rauf.

13 recordsLinked to original sources
PubMed2005

Glucose oxidase immobilization on a novel cellulose acetate-polymethylmethacrylate membrane.

Glucose oxidase (GOD) was immobilized on cellulose acetate-polymethylmethacrylate (CA-PMMA) membrane. The immobilized GOD showed better performance as compared to the free enzyme in terms of thermal stability retaining 46% of the original activity at 70 degrees C where the original activity corresponded to that obtained at 20 degrees C. FT-IR and SEM were employed to study the membrane morphology and structure after treatment at 70 degrees C. The pH profile of the immobilized and the free enzyme was found to be similar. A 2.4-fold increase in Km value was observed after immobilization whereas Vmax value was lower for the immobilized GOD. Immobilized glucose oxidase showed improved operational stability by maintaining 33% of the initial activity after 35 cycles of repeated use and was found to retain 94% of activity after 1 month storage period. Improved resistance against urea denaturation was achieved and the immobilized glucose oxidase retained 50% of the activity without urea in the presence of 5M urea whereas free enzyme retained only 8% activity.

Biosensing Techniques↗
PubMed2005

Electrochemical approach of anticancer drugs--DNA interaction.

The interaction of drugs with DNA is among the most important aspects of biological studies in drug discovery and pharmaceutical development processes. In recent years there has been a growing interest in the electrochemical investigation of interaction between anticancer drugs and DNA. Observing the pre and post electrochemical signals of DNA or drug interaction provides good evidence for the interaction mechanism to be elucidated. Also this interaction could be used for the quantification of these drugs and for the determination of new drugs targeting DNA. Electrochemical approach can provide new insight into rational drug design and would lead to further understanding of the interaction mechanism between anticancer drugs and DNA.

Antineoplastic Agents↗
PubMed1996

Nephritogenicity and alpha-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane.

Nephritogenicity (anti-GBM-nephritis-inducing activity) and alpha-chain composition of globular-do-main (NCI) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different alpha chains of type IV collagen. A purified nephritogenic fraction from renal BM contained alpha 1-alpha 6(IV)NCI, whereas a non-nephritogenic fraction contained only alpha 1-alpha 2(IV)NCI. Renal and pulmonary NCI had strong nephritogenic activity: placental NCI had weak activity. The renal and pulmonary fractions contained alpha 1-alpha 6(IV)NCI, and the placental fraction had a large amount of alpha 1-alpha 2(IV)NCI and a very small amount of alpha 3-alpha 6(IV)NCI. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained alpha 1-alpha 5(IV) chains, but not the alpha 6(IV) chain. The absence of alpha 6(IV) chain in glomerular BM in bovine and in humans indicates that alpha 6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of alpha 3-alpha 5(IV)NCI.

Animals↗
PubMed1992

Isologous monoclonal antibodies can induce anti-GBM glomerulonephritis in rats.

Injection of isologous monoclonal antibodies (SR2, SR3) caused anti-glomerular basement membrane antibody-induced glomerulonephritis (anti-GBM nephritis) in WKY/NCrj rats. The antibodies were obtained from hybridoma cells derived from fusion of the spleen of a nephritic WKY/NCrj rat injected with rat solubilized renal basement membranes with adjuvant, and mouse SP2-myeloma cells. They belonged to the rat IgG2a subclass and bound to rat kidney in a linear pattern along the glomerular and tubular basement membranes. Histological changes in glomeruli were detected at day 1 after the injection; proteinuria with haematuria appeared on day 2; and proteinuria became severe and reached a plateau by day 5. These results demonstrate that anti-GBM nephritis can even be induced by an isologous monoclonal antibody and that the rat IgG2a subclass is at least nephritogenic. The experimental model of anti-GBM nephritis with isologous monoclonal antibodies makes it possible and easier to analyse further the mechanism of anti-GBM nephritis.

Animals↗
PubMed1991

Structural homology between hemagglutinin (HA) of measles virus and the active site of long neurotoxins.

The amino acid sequence of a carboxy terminal domain corresponding to the end of the outer envelope projection of the hemagglutinin glycoprotein (HA) of measles and subacute sclerosing panencephalitis viruses has a high degree of homology with the active domain of long neurotoxins, which specifically binds to the nicotinic acetylcholine receptor. The homology in amino acid sequence of HA to this domain of neurotoxin, as well as native alpha-bungarotoxin (BTx), was confirmed by the following evidence: a) rabbit anti-HA monospecific sera reacted with BTx in ELISA, b) HA dose-dependently blocked the binding of radio-labeled BTx in competitive radioimmunoassay, and c) antibody to a synthetic peptide of the active domain of BTx precipitated HA in radioimmunoprecipitation. In addition, SSPE patients had significantly high titers of antibody to BTx than healthy children who had been previously infected with measles. This epitope of HA may play an important role in the transsynaptic spreading of the virus in the brain.

Adolescent↗
PubMed1990

Preliminary experience with extracorporeal shock-wave lithotripsy monotherapy and bladder calculi.

During the period November 1988 to April 1989, eleven patients with bladder calculi, seen at our Centre, were offered ESWL therapy. However, only eight patients underwent ESWL. One patient passed his stone spontaneously, one requested endoscopic lithotrity as the initial mode of therapy and one underwent transurethral resection of the prostate and simultaneous endoscopic lithotrity of his bladder calculus. This series, though admittedly, small, nevertheless serves to introduce a safe and expeditious modality for the removal of bladder calculi. A high degree of patient acceptance and no treatment failures embolden us to advocate this modality as initial therapy for bladder calculi.

Adult↗
PubMedDate not supplied

An epidemological study of smoking at Abbottabad.

BACKGROUND: Smoking is a type of environmental pollution and is injurious to health. We conducted an epidemiological study of different forms of smoking like cigarette, Hookah (Hubble-Bubble) and cigar in Abbottabad. METHODS: 600 randomly selected subjects residing in different localities of Abbottabad were included. A specifically designed questionnaire was filled. RESULTS: Out of the study population 200 (33%) were found to be smoking. CONCLUSIONS: A significant proportion of Abbottabad population smokes. This city is supposed to be a relatively less polluted hill station where a lot of people come for vacations.

Humans↗
PubMedDate not supplied

Effect of oral and injectable contraceptives on serum calcium, magnesium and phosphorus in women.

BACKGROUND: This study aims at evaluating serum calcium, magnesium and phosphorus levels in women taking oral and injectable contraceptives. METHODS: Serum calcium, magnesium and phosphorus were measured in 50 women taking oral contraceptives (Lofeminal) and 50 women taking injectable contraceptive (Depo-medroxy progesterone acetate and Norigest). These women were used as controls before starting these contraceptives. RESULTS: There was significant decrease in serum levels of calcium, magnesium and phosphorus in women taking oral contraceptives but there was significant increase in these minerals in women taking injectable contraceptives. CONCLUSIONS: It is suggested that these contraceptives should be used with due care and with proper investigations of the women before and during the therapy.

Adult↗
PubMedDate not supplied

Effect of oral and injectable contraceptives on serum electrolytes, weight and blood pressure.

BACKGROUND: Since the introduction of oral and injectable contraceptives, many metabolic side effects have been reported. The use of oral contraceptive has been shown to be associated with increased level of serum electrolytes, but decrease in levels of serum electrolytes has been reported in women using injectable contraceptives. This study evaluated serum electrolytes, weight and blood pressure in women taking oral and injectable contraceptives because these changes have adverse effects on the health and economy of the subject. METHODS: Serum electrolytes, weight and blood pressure were measured in 50 women taking oral contraceptive (Lofeminal) and 50 women taking injectable contraceptives (Depomedroxy progesterone acetate and Norigest). The same women served as control before starting these contraceptives. RESULTS: There was a significant increase in serum sodium and chloride but significant decrease in serum potassium and bicarbonate level in women taking oral contraceptive. There was non-significant decrease in serum electrolytes in women taking injectable contraceptives. There was significant increase in weight and blood pressure of women taking these contraceptives.

Adult↗
PubMedDate not supplied

Plasma concentrations of urea and creatinine in primary school children in Ujung Pandang.

A study of plasma concentrations of urea creatinine in 202 primary school children aged between 6 to 15 years was carried out in Ujung Pandang from November 1, 1988 through February 28, 1989. Sampling was done using multi-stage random sampling method. Plasma urea concentrations were not affected by sex, age and nutritional status. Normal distribution of plasma urea concentrations in P2.5 and P97.5 were 8.13 mg/dl and 24.09 mg/dl respectively (95% confidence level). There was no difference of creatinine concentration between the two sexes. The overall mean creatinine concentrations was significantly higher in the well-nourished group (0.73 +/- 0.081) mg/dl) as compared to PEM group (0.63 +/- 0.066 mg/dl). This study revealed a correlation between age and plasma creatinine concentrations in the well-nourished (r = 0.46, p less than 0.01) as well as in PEM (r = 0.37, p less than 0.01) group. Hence, normal distribution of plasma creatinine concentrations should be base on values in each age group. This study showed no correlation between plasma urea and creatinine concentrations.

Adolescent↗