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Biomedical subjects

S Rico

Publications and source records attributed to S Rico.

12 recordsLinked to original sources
PubMed1999

Interlaboratory study comparing the microbiological potency of spiramycins I, II and III.

An interlaboratory study has been performed to determine the relative potencies of spiramycins (SPMs) I, II and III by diffusion or/and turbidimetric assays with Bacillus subtilis or Staphylococcus aureus as the test organisms. Six laboratories from three countries participated. Experimental procedures were according to the European Pharmacopoeia, 3rd ed. The activity of SPM I is markedly higher than that of SPM II and III. By diffusion, the activities of SPM II and III relative to SPM I were found to be 57 and 72%, respectively. The interlaboratory relative standard deviations (RSD) varied from 3.6 to 16.3%. By turbidimetry, the activities of SPM II and III relative to SPM I were found to be 45 and 52%, respectively. The interlaboratory RSD values varied from 2.6 to 7.7%. The results of the study were analyzed according to the ISO 5725-2 guidelines to determine the repeatability, the between-laboratory and the reproducibility variances of both methods.

Aminoglycosides↗
PubMed1998

Neomycin: microbiological assay or liquid chromatography?

In a multicentre study involving six laboratories, a microbiological assay was performed on three neomycin samples containing respectively, 0.12, 2.1 and 11% (m/m) of neomycin C, as well on a pure neomycin C sample. The potency was determined according to the European Pharmacopoeia method but using a neomycin B base standard. The relative standard deviations between laboratories (RSD) on the potencies varied from 4.8 to 50%, depending on the sample examined. The RSD increased with the neomycin C content of the samples and the highest RSD values were observed for the pure neomycin C sample. The activity of neomycin C relative to neomycin B was found to be 62% by diffusion (RSD:41%) and 56% by turbidimetry (RSD: 50%). This confirmed that the presence of neomycin C in a neomycin sample influences the reproducibility of the microbiological assay. T estimate the influence of this effect on official standard, their composition was verified by liquid chromatography. The neomycin C base content of the standards varied between 0.4 and 5.8% (m/m). Based on the results obtained and on formerly published reports discussing problems encountered with microbiological assay of neomycin, it is proposed to introduce liquid chromatography in official monographs to replace microbiological assay.

Anti-Bacterial Agents↗
PubMed1985

[Cardiorespirography of the newborn infant at 12 hours of life].

The introduction of the cardiorespirogram more than twenty years ago provided a useful continous noninvasive monitoring technique of heart and respiratory functions. However, there are only few studies about recording of these parameters and the topic is often partially discussed. Thus, the aim of this paper is to analyze the cardiorespirograms from newborn infants, divided into three groups: 50 normal fullterm infants, 28 preterm and 16 fullterm low birth weight infants. The age at monitoring was 12 hours. The 50 normal newborns reveal patterns similar to those described by other authors. The preterm and fullterm low birth weight infants show a significantly higher baseline with less variability and reactivity, data which lead us to bear in mind gestational age and birth weight to evaluate the cardiorespirogram.

Heart Rate↗
PubMed1980

[Complete triploidy in a liveborn premature (author's transl)].

Authors describe the clinical, pathological and cytogenetic data of a polymalformed premature with a complete a triploidy 69,XXY). This rare condition (30 cases in the literature) may be suspected in a premature with broad posterior fontanella, eye and ear anomalies, syndactily between IV and III fingers/toes, intersexual status and mielomeningocele. Frequently the placenta presents a molar or micromolar degeneration.

Abnormalities, Multiple↗