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S Roggero

Publications and source records attributed to S Roggero.

24 records · Page 2Linked to original sources
PubMed1989

Generation and selection of monoclonal antibodies identifying surface molecules discriminating between fetal and adult fibroblasts.

The present report describes the reactivity of two murine monoclonal antibodies, i.e., CB11 and CB43, the first obtained by using embryonic material as immunizer and the second elicited against a human cell line. These reagents recognize two distinct molecules marking a peculiar differentiation stage on fetal/embryonic fibroblasts. The first structure is a single chain of 14.5 kd, the second one a heterodimer of 40-80 kd. Even if these reagents are not strictly fibroblast restricted, translineage expression does not diminish the medical relevance of the reagents; in fact, they may have application in prenatal diagnostics and amniocentesis testing, in which it is necessary to discriminate between fetal and maternal cells.

Adult↗
PubMed1989

Phenotypic and functional analysis of circulating phagocytic cells in IgA nephropathy patients.

We analysed the possible mechanisms responsible for the functional abnormalities reported on phagocyte cell function in IgA nephropathy patients. The oxidative metabolism by the superoxide anion ferricytochrome C reduction test on monocytes and the chemiluminescence response on neutrophils was examined in 32 patients. Moreover the expression of cell membrane structures involved in immune response and phagocytosis (i.e. Fc receptors and HLA class II antigens) was evaluated cytofluorimetrically using specific monoclonal antibodies. Our own results did not show striking differences in oxidative potential and phenotype of patients' phagocyte cells as compared with healthy controls. However a HLA class II-associated structure was found to be quantitatively more expressed in patients' monocytes than in normal controls (P less than 0.005). These findings suggest that the defective phagocyte system function reported in IgA nephropathy patients is not simply due to a loss of cell surface receptors, nor to abnormalities in intracellular metabolic pathways implying oxygen consumption. The increase in expression of some HLA class II structures, often associated with the presence of circulating IgA immune complexes, probably reflects a protracted immunological stimulation.

Adolescent↗
PubMed1988

Exercise-induced microalbuminuria in diabetes is associated with the urinary excretion of cationic proteins.

Urinary protein components, highly cationic in nature (pI 10.9), with apparent molecular weights of 65 kD, 45 kD, and 28 kD were detected in type I diabetic patients before (2/7), during (4/7) and after (1/7) exercise. Based on the apparent isoelectric point and molecular weights, the urinary cationic components in the urines of type I diabetic patients were superimposable to purified cationic proteins derived from human platelets. Furthermore, they also shared common antigenic components as evidenced by the ability of a goat anti-human platelet-derived cationic protein serum to immunoprecipitate the 45 kD protein fraction. The latter induced vascular permeability when injected intradermally in the rabbit's skin. The occurrence of urinary cationic proteins was associated with the significant increase in albumin excretion rate (AER) in diabetic patients. No urinary cationic components were ever detected in the urines of 6 healthy subjects before, during, or after exercise. The possible pathogenetic relevance of the urinary excretion of cationic proteins in diabetic AER is discussed.

Adolescent↗
PubMed1984

Generation and characterization of murine monoclonal antibodies against HLA Class II molecules.

HLA Class II antigens (human la) are coded by Major Histocompatibility Complex and play important biological roles in health and disease. In this report we describe the generation and characterization of nine murine monoclonal antibodies (MoAbs) specific for determinants localized on the human la molecules. The reactivity of these MoAbs inferred from serological analysis along with the data obtained from biochemical characterization of the target structures allowed a classification of these reagents as monomorphic and polymorphic. Two monomorphic MoAbs, identifying different subsets of human la molecules, were studied in detail.

Animals↗
PubMedDate not supplied

Waiting list for kidney transplant. Some patients wait too long.

The selection of a kidney graft recipient should be made not only taking into account biological and clinical parameters, for assuring the maximum possible clinical success; the ethical objective to allow every patient equal opportunity of receiving a transplant should also be pursued. In every waiting list of transplant candidates a proportion of patients remains in the list for a particularly long time. The present analysis aimed to find out the factors associated with a prolonged waiting time, in order to allow the implementation of patient selection criteria able to balance unfavourable factors. The analysis of the waiting list of our kidney transplant centre allowed to observe that blood group 0, anti-HLA immunisation, presence of rare HLA antigens and, at a lesser extent, HLA homozygosity are associated with a longer waiting time for a kidney transplant.

Algorithms↗