Biomedical subjects

S Rust

Publications and source records attributed to S Rust.

28 records · Page 2Linked to original sources

PubMed1986

Glucose-6-phosphate-dehydrogenase (G6PDH) activity in the liver of golden hamsters--its dependence from estrous cycle and consequences on planning experiments.

Glucose-6-phosphate-dehydrogenase activity in the liver of female golden hamsters being in various stages of the estrous cycle were investigated. Animals of the pro- and diestrous stage showed a significantly higher activity than those of the met- and estrous stage. The interpretation of the results in test series has to consider this fact. Possibilities for it have been discussed.

Animals↗

PubMed1986

Detection of apolipoprotein E variants by an oligonucleotide "melting" procedure.

An oligonucleotide "melting" procedure was developed whereby we can monitor for a sequence heterogeneity in the gene for apolipoprotein E production. Two oligonucleotides were synthesized, one recognizing the common epsilon 3 allele but not the common epsilon 2 allele, the other recognizing the epsilon 2 allele but not the epsilon 3 allele. Samples from 15 subjects with different apolipoprotein E phenotypes as classified by isoelectric focusing were analyzed by this method for the presence of an Arg----Cys substitution in position 158 of the apolipoprotein E amino acid sequence. In 14 of these subjects the genotype determined by oligonucleotide melting agreed with the phenotype identified by isoelectric focusing. In one patient, however, whose phenotype was E2/2 by isoelectric focusing, the DNA hybridized with both oligonucleotides. We conclude that this patient has a mutation in one of his alleles for apolipoprotein E that differs from the frequently seen Arg----Cys change. The apolipoprotein E gene may thus be more heterogeneous than previously anticipated.

Alleles↗

PubMed1984

The effect of cimetidine treatment on ethanol formation in the human stomach.

Ethanol concentrations were determined in the gastric juice of 53 patients treated with a nasogastric tube. Significant ethanol concentrations ranging from 1 to 27 mmol/l were found in a subgroup of 29 patients receiving cimetidine (n = 22) or antacids (n = 7). The mean ethanol concentration in these patients was higher 1-2 h after a liquid meal (means +/- SEM, 6.95 +/- 2.6) than in the fasting state (3.44 +/- 2.1; p less than 0.05). In the second subgroup of 24 patients, who were not treated with cimetidine or antacids, ethanol concentrations in gastric juice of more than 1 mmol/l were found in 6 cases, with maximal ethanol values of 2.2 mmol/l. The alcohol concentration correlated significantly with the pH of the gastric juice. It is assumed that an increase in the yeast and/or bacterial population in the stomach due to the reduction of gastric acidity induced by cimetidine or antacids is responsible for the enhanced production of ethanol.

Antacids↗

PubMed1972