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Biomedical subjects

S S Scott

Publications and source records attributed to S S Scott.

17 recordsLinked to original sources
PubMed2001

Recombinant expression and purification of human androgen receptor in a baculovirus system.

A full-length human androgen receptor (hAR) cDNA was used to produce recombinant baculovirus. Spodoptera frugiperda (Sf9) cells infected with this virus expressed protein with an N-terminal hexahistidine tag (His(6)-hAR) in soluble and insoluble forms. The soluble cytosolic His(6)-hAR demonstrated similar association and dissociation half-times for mibolerone, similar binding affinity for mibolerone, and similar steroid specificity as bona fide AR. Under native conditions, the soluble cytosolic His(6)-hAR was purified to apparent homogeneity in the presence of dihydrotestosterone, using metal ion affinity chromatography. The insoluble pellet fraction was solubilized with strong denaturant 6 M guanidine HCl, and His(6)-hAR was purified from it in the presence of 6 M guanidine HCl. Both the solubilized crude pellet fraction and the solubilized/purified His(6)-hAR could be renatured to bind mibolerone. The baculovirus system will therefore provide an efficient means for producing hAR for ligand-binding assays, as well as purifying hAR for detailed molecular analyses.

Animals↗
PubMed2000

Temperature measurement in critically ill adults: a comparison of tympanic and oral methods.

BACKGROUND: Despite increasing use of tympanic thermometers in critically ill patients who do not have a pulmonary artery catheter in place, variations in measurements obtained with the thermometers are still a problem. OBJECTIVE: To compare the range of variability between tympanic and oral electronic thermometers. METHODS: Subjects were a convenience sample of 72 patients admitted to a 24-bed adult medical-surgical intensive care unit. For each patient, temperatures were measured concurrently (within a 1-minute period) with an oral (Sure Temp 678) thermometer, a pulmonary artery catheter (Baxter VIP Swan-Ganz Catheter), and 2 tympanic (FirstTemp Genius II and ThermoScan Ear Pro-1) thermometers. Each subject was used up to 3 times for data collection. Measurements obtained with the oral and tympanic thermometers were compared with those obtained with the pulmonary artery catheter. Nonparametric analysis of data was used. RESULTS: The magnitude of error for the ThermoScan tympanic thermometer differed significantly from that of the Genius II tympanic thermometer and the SureTemp oral thermometer (P < .001). Application of the Bland and Altman method to frame the data on the basis of an accuracy tolerance zone of +/-0.5 degrees C indicated variability with both the oral and tympanic methods. The overall degree of variability was lower for the oral thermometer. CONCLUSIONS: Oral thermometers provide less variable measurements than do tympanic thermometers. Use of oral thermometry is recommended as the best practice method for temperature evaluation in critical care patients when measurement of core temperature via a pulmonary artery catheter is not possible.

Adult↗
PubMed1999

Temperature measurement in critically ill orally intubated adults: a comparison of pulmonary artery core, tympanic, and oral methods.

OBJECTIVE: Core temperature measurement using a pulmonary artery (PA) catheter is considered the gold standard for measuring temperatures in critically ill patients. The objective of this study was to compare oral and tympanic temperature measurements (in both the oral and core equivalence modes) against PA core temperature measurements to determine which method was the most accurate and reliable in the absence of a PA catheter. DESIGN: Prospective, descriptive comparative analysis. PATIENTS: Convenience sample of 102 critically ill orally intubated patients with a PA catheter in place. SETTING: A 24-bed medical/surgical/trauma intensive care unit in a university-affiliated medical center. INTERVENTIONS: Four experienced intensive care unit nurses were trained in the use of temperature measurement with the oral, tympanic (both core and oral equivalence modes were used), and PA core methods. Simultaneous temperature measurements were then taken once in each subject using each method. The potential covariates that were analyzed were mean blood pressure, patient acuity using the Simplified Acute Physiology Score II, age, sex, ambient room temperature, and ventilator circuit temperature. MEASUREMENTS AND MAIN RESULTS: The training period indicated that it took more time to train experienced nurses in the use of tympanic thermometry than oral thermometry. Descriptive statistics were the following: core, x = 37.33 (SD = 0.89); oral, x = 37.18 (SD = 0.92); tympanic oral, x = 36.80 (SD = 0.93); and tympanic core, x = 37.12 (SD = 1.0). Bias averages were calculated and were significantly different from 0 for all three methods (oral-PA core, -0.15 [SD = 0.36]; tympanic core-PA core, -0.11 [SD = 0.57], tympanic oral-PA core, -0.52 [SD = 0.53]), indicating that there is some degree of decreased accuracy associated with each method when compared with PA core. However, scatter plots using the Bland and Altman methodology (Altman DG, Bland JM: Practical Statistics for Medical Research. London, Chapman and Hall, 1991) illustrate that the greatest variability is associated with the tympanic method. CONCLUSIONS: Temperature measurement is an important piece of clinical data in a critically ill patient population. We found oral thermometry to be the most accurate and reproducible method when a PA core measurement was not available.

APACHE↗
PubMed1998

Influence of port site on central venous pressure measurements from triple-lumen catheters in critically ill adults.

BACKGROUND: Measurements of central venous pressure are generally obtained through one of the three ports of centrally placed triple-lumen catheters. However, no scientifically based literature is available that guides clinical practice and indicates which of the lumens is most appropriate for obtaining these measurements. OBJECTIVE: To determine if a difference exists between measurements of central venous pressure obtained via the proximal, medial, and distal ports of a triple-lumen catheter. METHODS: Measurements of central venous pressure in 48 adult ICU patients were obtained via each of the three ports of a triple-lumen catheter. Catheters were placed in either the right or left subclavian vein or the right or left internal jugular vein. The flush system was single-transducer, trifurcated pressure tubing system. Data were evaluated for variation among the three ports of the catheter for readings taken at a single point. RESULTS: A repeated-measures analysis of variance showed significant differences across port sites. Post hoc univariate F tests showed significant differences between the proximal and distal ports and between the medial and distal ports. In some patients, the difference between central venous pressure obtained from the distal port and pressure obtained from the proximal or the medial port may be clinically significant. CONCLUSION: Because measurements of central venous pressure may not always be comparable for all three ports of a triple-lumen catheter, care should be taken to distinguish when changes in pressure readings are a result of a change in port site rather than in the patient's condition.

Adult↗
PubMed1995

Child abuse potential inventory and parenting behavior: relationships with high-risk correlates.

The primary purpose of this research was to examine the construct validity of the Child Abuse Potential Inventory by comparing maltreating and high-risk parents' CAP Inventory abuse scores to their behavior during interactions with their children. A second purpose was to determine the degree to which CAP Inventory scores and parenting behavior were related to several known correlates of abuse, as measured by parent and teacher reports. Participants (n = 41) included abusive and high-risk parents and their children referred to a treatment group. Correlational analyses revealed that CAP Inventory scores and observed parenting style yielded highly related findings, supporting construct validity of the CAP Inventory. However, the CAP Inventory and observed behavior index showed a different pattern of relationships to the risk correlates. Implications for assessment of risk status are discussed and recommendations are provided for continued research.

Adult↗
PubMed1991

A change in legal status: an overlooked dimension in the transition to higher education.

The transition from high school to college entails a change in legal status for individuals with learning disabilities. As students leave high school and the dominion of P.L. 94-142, they enter a setting structured by Section 504 of the Rehabilitation Act of 1973. How does this change in legal status affect student rights and responsibilities? Do students with learning disabilities need specific skills to facilitate their legal transition into higher education? Is current transition programming addressing To investigate these questions, basic provisions of P.L. 94-142 and Section 504 were reviewed. Implications of the change in legal status were discussed, and existing transition programs were examined. Guidelines are proposed for incorporating legal transition skills in future transition programming.

Adolescent↗
PubMed1989

Vitamin B6 repletion in cirrhosis with oral pyridoxine: failure to improve amino acid metabolism.

This study evaluated the effect of daily oral pyridoxine supplementation in patients with cirrhosis. Eight subjects were treated with 25 mg of pyridoxine for 28 days. Before and after the supplementation period, B6 status was assessed by measuring fasting plasma vitamer levels and response to a 25 mg oral pyridoxine load. In addition, a 24-hr urine collection was analyzed during each load study for B6 metabolites. The data indicated that supplementation achieved repletion of peripheral B6 stores, as evidenced by: (i) a significant (p less than 0.005) rise in fasting plasma pyridoxal phosphate after supplementation (mean +/- S.D. = 56.8 +/- 30.5 nmoles per liter) as compared to initial levels (17.0 +/- 17.8 nmoles per liter); (ii) a higher (p less than 0.05) percentage excretion of the pyridoxine load as urinary 4-pyridoxic acid (31.0 +/- 9.3%) compared to the initial load (19.6 +/- 5.8%), and (iii) a postsupplementation area under the plasma concentration vs. time curve for pyridoxal phosphate (377 +/- 529 nmoles.hr per liter), which was decreased (p less than 0.005) from the presupplementation value (934 +/- 756 nmoles.hr per liter). The postsupplementation fasting plasma pyridoxal phosphate concentrations were within the normal range. The consequences of B6 repletion on amino acid metabolism were measured by oral protein loads (n = 4) or oral methionine loads (n = 4). No significant changes were observed for methionine or any other amino acid in regard to plasma fasting concentration, peak concentration or AUC. Although the vitamin B6 deficiency of cirrhosis was corrected by daily oral pyridoxine supplementation, there was apparently no improvement in the deranged amino acid metabolism.

Amino Acids↗
PubMed1987

Depressed leukotriene B4 chemotactic response of neutrophils from localized juvenile periodontitis patients.

Previous studies have demonstrated that certain local juvenile periodontitis (LJP) patients possess neutrophils with an intrinsic chemotactic defect to the peptide chemoattractants f-met-leu-phe (FMLP) and C5a. In this investigation, the in vitro response of neutrophils to the chemotactic agent leukotriene B4 (LTB4) was examined. Those LJP patients who possessed defective chemotactic responses to FMLP and endotoxin activated serum (EAS) also had impaired chemotaxis to LTB4. Exogenous LTB4 failed to augment the impaired response to FMLP. Evidence for a global membrane defect in LJP neutrophils resulting in hyporesponsiveness to chemically diverse chemotactic agents is presented.

Adult↗
PubMed1984

Phase resetting properties of cardiac pacemaker cells.

Aggregates of heart cells from chicken embryos beat spontaneously. We used intracellular microelectrodes to record the periodic behavior of the membrane potential that triggers the contractions. Every 5-12 beats, a short current pulse was applied at various points in the cycle to study the phase-dependent resetting of the rhythm. Pulses stronger than 2.5 nA caused the final rhythm to be reset to almost the same point in the cycle regardless of the phase at which the pulse was applied (type zero resetting). Pulses of less than or equal to 1 nA only caused a slight change of the phase. Increasing current intensities to between 1 and 2.5 nA gave rise to an increasing steepness in a small part of the phase-response curve. The observation of type zero resetting implies the existence of a critical stimulation that might annihilate the rhythm. Although we did find a phase at which more or less random responses occurred, the longest pause in the rhythm was 758 ms, 2.4 times the spontaneous interval. This suggests that the resting membrane potential was unstable, at least against the internal noise of the system. The conclusions are discussed in terms of the concepts of classical cardiac electrophysiology.

Action Potentials↗
PubMed1983

Multiple fimbrial haemagglutinins in Serratia species.

Fifty-six strains from nine Serratia species, grown under a variety of cultural conditions, were examined in rocked-tile tests for the presence of haemagglutinins (HAs) and with the electron microscope for fimbriae. All strains were haemagglutinating; most (71%) formed two or three of the different kinds of HA detected. These were: (i) a mannose-sensitive HA (MS-HA); (ii) a mannose-resistant, klebsiella-like HA (MR/K-HA); (iii) two mannose-resistant, proteus-like HAs (MR/P-HA), one of which showed broad-spectrum HA activity against different species of erythrocytes and the other narrow-spectrum HA activity. Five types of fimbriae associated with the different HAs were observed. This description of the fimbrial HAs in nine species of Serratia is the most comprehensive so far produced and should provide a basis for future studies directed towards elucidating the ecological role of the adhesins in in-vivo colonization by serratiae as commensals or pathogens.

Fimbriae, Bacterial↗
PubMed1981

Hemagglutinins and fimbriae of Providencia spp.

A series of 25 strains of Providencia spp. produced at least five different patterns of hemagglutination as judged by results from hemagglutination tests in the presence and absence of D-mannose and at least six distinct types of fimbriae as seen from observations with the electron microscope.

Fimbriae, Bacterial↗
PubMedDate not supplied

Definition and documentation: theory, measurement, and the courts.

Definition of terms and the selection criteria for operationalizing the standard of disability are critical to accessing accommodations. The purpose of this article was to examine the research surrounding definitiorns and selection criteria for learning disabilities and attention-deficit/hyperactivity disorder at the postsecondary level. Implications from and for the courts follow a critical analysis of the professional literature. The need for researchers to utilize theory-based models to investigate the multivariate relationships between and within constructs underlying current definitions and eligibility criteria is also disscussed.

Attention Deficit Disorder with Hyperactivity↗
PubMedDate not supplied

Meeting the evolving education needs of faculty in providing access for college students with LD.

Faculty play an essential role in providing access for college students with LD. Though many recommendations exist in the literature for educating faculty about their roles regarding students with LD, it is unclear whether these strategies are actually addressing faculty needs. To examine this issue, the evolving role of faculty is discussed. Current practices in faculty education pertaining to college students with LD are reviewed. Discrepancies between the evolving faculty role and current faculty education practices are examined. Guiding questions are proposed for expanding faculty education efforts and models to keep pace with the evolving faculty role in providing access for college students with LD.

Adult↗
PubMedDate not supplied

Coming to terms with the "otherwise qualified" student with a learning disability.

Section 504 of the Rehabilitation Act of 1973 ensures all "otherwise qualified" individuals with handicaps the right to higher education. Federal regulations implementing the broad principles of Section 504 address only obliquely the interpretation of the construct "otherwise qualified." The determination of the qualified individual with a handicap in higher education entails unique consideration when applied to students with learning disabilities. In a setting traditionally based on academic merit and competition, the student with a learning disability requires accommodation of the learning process. Postsecondary institutions are faced with the task of determining the qualified student while maintaining academic standards. To clarify this process, existing tenets of the construct "otherwise qualified" were examined. Issues in implementing the law with students with learning disabilities were discussed, and current practice in addressing the issues was evaluated in light of legal principles. Guidelines were proposed for implementing the construct "otherwise qualified" with students with learning disabilities in higher education.

Persons with Disabilities↗
PubMedDate not supplied

Determining reasonable academic adjustments for college students with learning disabilities.

Federal law and the implementing regulations have established that appropriate academic adjustments must be provided to college students with learning disabilities in order to ensure meaningful access to higher education. However, federal law is necessarily broad and offers no guidance in how to consider accommodation requests for individual students in specific contexts. To address this issue, legal tenets from federal and case law were examined, and existing guidelines for weighing accommodation requests were reviewed. Recommendations were proposed for determining appropriate academic adjustments for college students with learning disabilities, and persisting issues were discussed.

Persons with Disabilities↗