Biomedical subjects

S S Shchelokova

Publications and source records attributed to S S Shchelokova.

4 recordsLinked to original sources

PubMedDate not supplied

[Oospora lactis strain actively producing lipase].

Morphological, cultural and physiological properties of the fungus Oospora lactis producing lipase are described. The activity of lipase depends on the composition of a growth medium, pH, temperature, the duration of cultivation. Optimum conditions for lipase biosynthesis by the fungus have been found. The strain produces extracellular and endocellular lipases during submerged cultivation in the optimum growth medium at a high rate.

Culture Media↗

PubMedDate not supplied

[Torula thermophila, strain UzPT-1 a thermophilic producer of proteolytic enzymes].

The properties of the thermophilous fungus Torula thermophila producing proteolytic enzymes are described. Oprimal conditions for its cultivation are established. The maximum action of the preparation isolated from the culture liquid filtrate is at pH 9-11. The temperature maximum of the enzyme activity is 70 degrees C.

Cryptococcus↗

PubMedDate not supplied

[Effect of different nitrogen sources on the biosynthesis of lipase by Oospora lactis].

The effect of inorganic and organic nitrogen compounds on the synthesis of biomass and extracellular lipase by Oospora lactis was studied. Among the inorganic nitrogen sources ammonium sulphate and ammonium secondary phosphate and among the organic nitrogen sources yeast autolysate proved to be most beneficial for the lipase synthesis. Lipase activity and biomass accumulation in the medium containing yeast autolysate were greater than in the media containing the above ammonium salts. Lipase synthesis reached maximum in the nutrient medium containing yeast autolysate (0.7%) and ammonium sulphate (0.3%).

Ammonium Sulfate↗

PubMedDate not supplied

[Effect of tweens on the lipase activity of Oospora lactis].

Oospora lactis was found to use Tweens 20, 40, 60 and 80 as a source of carbon in a nutrient medium for lipase biosynthesis. The highest production of lipase was registered in the medium with Tween-80. The lipase activity of the culture increased if even small quantities of Tweens 40, 60 and, particularly, 80 (0.05--1.0%) were added to the medium containing cottonseed oil. All the tested Tweens stimulating the biosynthesis of lipase were also, at a particular concentration, the inhibitors of this enzyme.

Culture Media↗