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Biomedical subjects

S Sagami

Publications and source records attributed to S Sagami.

At least 37 records · Page 2Linked to original sources
PubMed1987

Ultrastructural studies of the skin and cultured fibroblasts in I-cell disease.

The skin and cultured fibroblasts from a patient with I-cell disease were examined by electron microscopy. Multiple vacuolations were seen in fibroblast and/or histiocyte-like cells, secretory cells of eccrine glands, and Schwann cells of the skin. Vacuolar inclusions were single membrane-limited, and contained a few reticulo-floccular and vesicular materials, endothelial cells of the dermal capillaries contained other types of inclusions, which were also membrane-limited, more electron dense, and multivesicular. The epidermis and pilosebaceous appendages seemed to be normal. Cultured skin fibroblasts contained prominent inclusions which varied in size and morphology. Acid phosphatase activity was seen in some of those inclusions, indicating their derivation from lysosomes. These findings suggest that the I-cell disease is a type of lysosomal storage disease and that electron microscopic examination of normal-appearing skin in this disease may contribute to the diagnosis.

Cells, Cultured↗
PubMed1986

Bowen's disease associated with multiple myeloma.

The occurrence of Bowen's disease on unexposed area of the body in a 68-year-old man with multiple myeloma is reported. Not only non-reticular but also reticular malignancy should be taken into consideration as internal malignancy associated with Bowen's disease. The previous reports of Bowen's disease associated with reticular malignancy are also reviewed.

Aged↗
PubMed1985

Electron microscopic study on Langerhans cells and related cells in lymph nodes of DNCB-sensitive mice.

To understand contact hypersensitivity, it is important to know the kinetics of Langerhans cells (LC) and related cells in the lymph node (LN), as well as in the skin. For this purpose, we tried experimentally to induce increased numbers of LCs, Birbeck granule-like structure (BgS)-containing cells, and interdigitating reticulum cells (IDC) in DNCB-sensitive mice and studied them by means of electron microscopy with the following results: (1) cytologically, LC, BgS-containing cells and IDC were closely related; (2) BgS seemed to arise from rough-surfaced endoplasmic reticulum (r-ER), and BgS-containing cells were midway in nature between LC and IDC from the morphological view point. From these findings, it appears that IDC, BgS-containing cells, and LCs were simultaneously involved in the contact hypersensitivity reactions of LNs.

Animals↗
PubMed1985

Increased Langerhans cells and related cells in mesenteric lymph nodes of DNCB-sensitive mice.

We used electron microscopy to study mesenteric lymph nodes (LNs) of 2,4-dinitro-1-chlorobenzene(DNCB)-sensitive mice. It was found that Langerhans cells (LCs) and cells containing Birbeck-granule-like structures (BgSs) increased in number only after challenge with DNCB. This increase reached a peak 4 h after challenge. LCs and BgS cells were only found in the paracortical area of LNs. There were no appreciable differences in the morphology of LCs, BgS cells, and interdigitating reticulum cells (IDCs), except for the presence or absence of Bgs or BgSs. No LCs, BgS cells, or IDCs were found in the marginal sinus of mesenteric LNs or in the surroundings of afferent blood vessels. These findings were in complete agreement with those obtained in our previous study of cutaneous LNs. From our findings and the results of previously published studies, we propose the following conclusions: first, LCs and BgS cells observed in mesenteric LNs are the product of the differentiation and maturation of precursor cells pre-existing in the paracortical area of LNs caused by DNCB stimulation. Second, the activated T lymphocyte to DNCB pre-exists in the paracortical area; thus, the DNCB used for challenge stimulates this T lymphocyte to release lymphokine, which then causes some precursor cells to differentiation into LCs and/or BgS cells. Finally, morphological findings suggest that precursor cells of LCs and BgS cells are probably IDCs.

Animals↗
PubMed1983

Induction of suppressor T cells to dinitrofluorobenzene contact sensitivity by application of sensitizer through Langerhans cell-deficient skin. II. Kinetics of the induction of suppressor T cells.

Tolerance to dinitrofluorobenzene (DNFB) contact sensitivity (DNFB CS) appeared in C3H/HeN mice within 2 days after application of DNFB on Langerhans cell-deficient tail skin. A single painting on tail skin led to complete prevention of DNFB CS for at least 14 days. Spleen cells from tolerant mice could transfer the suppression to syngeneic recipients, while the cells treated in vitro with anti-Thy 1.2 lost their suppressive activities completely. From these facts, this tolerance might be due to active suppression caused by suppressor T cells. These suppressor T cells were antigen-specific, because recipients exhibited normal CS to picryl chloride. Furthermore, the suppressor T cells were demonstrated to be afferent blockers in nature and their precursors were sensitive to cyclophosphamide. Therefore, the suppressor T cells induced by DNFB percutaneously administered through epidermal Langerhans cell-deficient region were thought to be generated in the same or in a very similar suppressor pathway as the intravenously administered dinitrobenzene sulfonate (DNBSO3), a soluble reactive dinitrophenyl congener.

Animals↗
PubMed1978

An ultrastructural study of localized lymphangioma circumscriptum.

An electron microscopic study was performed on the lymphatic vessel (lv) of localized lymphangioma circumscriptum (Lc), which was found clinically typical but histologically not associated with hemangioma and inflammation. The lv of Lc was morphologically divided into two types. The first type was situated at the upper dermis of the lesion and the other, at the deep dermis. The former had certain similarities to lv of experimentally induced lymph-stasis, whereas the latter was morphologically similar to lv of the normal skin. From the findings of the present study and those reported in literature, Lc could not be considered as a mere benign overgrowth of lv, but as one of pathological results induced by a long-standing lymph-stasis.

Adult↗