Some carry-over effects of pregnancy on post-partum ovarian function in the cow.
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Animals↗
Biomedical subjects
S Saiduddin
Publications and source records attributed to S Saiduddin.
Further consideration of systematic errors present in protein-ligand interactions.
Relatively minor systematic errors present during measurements of protein-ligand interaction can lead to large inaccuracies in the calculated values of the equilibrium dissociation constant and the total concentration of the binding protein. These errors, which include binding of the ligand to low affinity material and underestimation of bound ligand, cause the calculation of the concentration of free ligand at equilibrium to be overestimated. We report herein a model of ligand-protein binding which incorporates these errors into the mathematical formulation of the equilibrium binding equation. The effect of these errors on the Scatchard plot is presented.
Binding Sites↗
Assay and characterization of ovarian testosterone receptor.
Ovarian cytosol from immature, hypophysectomized rats was used to characterize the biochemical properties of ovarian androgen receptor and to develop a reliable and convenient assay procedure for its measurement in small quantities of tissue. The results show that Sephadex G-25 columns provide a rapid and reliable method to separate bound from free testosterone for assay of androgen receptor in the ovarian tissue. The binding affinity (KD = 0.6 nM) and sedimentation value (8S) for the ovarian androgen receptor are similar to other steroid receptors and is saturable. It is inactivated by heat and protease digestion and the binding is specific for potent androgens. The ovary contains approximately equal quantities of androgen and estrogen receptor. A physiological role for the presence of androgen receptor in the ovarian tissue is proposed.
Animals↗