Biomedical subjects
S Salamon
Publications and source records attributed to S Salamon.
Storage of ram semen.
Storage of ram semen in liquid and frozen state, the diluents used for both methods, processing, cooling, freezing and thawing of semen are reviewed. Factors influencing the fertility of stored semen and methods used for improvement are discussed, and fertility results of long-term frozen stored ram semen are also given.
Production and storage of goat semen for artificial insemination.
Environmental influences on reproduction and semen production in the buck, the problem of interaction between seminal plasma and egg yolk or milk constituents in diluent, liquid storage and processing of semen for freezing are discussed. A review is given on the use of frozen-thawed semen for artificial insemination (AI) in spontaneous and induced oestrus and factors influencing the fertility.
Liquid storage of ram semen: a review.
Research from 1930 to 1992 is reviewed with regard to storage of semen at reduced (0-5 degrees C or 10-15 degrees C) and at ambient temperatures. Diluents investigated have included synthetic buffers combined with sugars and egg yolk or its fractions, milk from various sources, glycine, and other substances. Irrespective of the diluent, dilution rate, temperature or conditions of storage, the spermatozoa deteriorate with time of storage. Changes include reduction in motility and morphological integrity of spermatozoa, accompanied by a decline in their survival in the female reproductive tract, reduction in fertility and increased embryonic loss. In critical studies, fertility declined rapidly when semen stored for more than 24 h was used for cervical insemination, but after intrauterine insemination some spermatozoa maintained their fertilizing capacity up to 10 days. Laparoscopic intrauterine or transcervical inseminations could be the means of improvement of fertility. These methods may eliminate the problem of sperm transport through the cervix and ageing of spermatozoa in the reproductive tract, thereby improving fertilization of ova and reducing embryonic loss.
Frequent semen collection and sperm reserves of the male Angora goat (Capra hircus).
Semen was collected from six mature and sexually rested Angora bucks at one-hour intervals five times a day on each of 5 consecutive days in the breeding season. There was a marked decline in semen volume (P less than 0.001), sperm concentration (P less than 0.05) and number of spermatozoa (P less than 0.001) on consecutive days. Successive ejaculates within days differed only in number of spermatozoa (P less than 0.001). The following year at the beginning of the breeding season, the weights of testes and epididymides and the reserves of spermatozoa in these parts were examined after slaughter of the six bucks. The mean number of spermatozoa in the paired testes, capita, corpora and caudae of the epididymides were (22.8 +/- 1.24) x 10(9), (9.4 +/- 1.19) x 10(9), (3.4 +/- 0.22) x 10(9) and (35.0 +/- 2.21) x 10(9), respectively. Epididymal reserves of spermatozoa were correlated with testicular weight (r = 0.50, P = 0.01) and number of spermatozoa in the testes (r = 0.42, P = 0.07), but not with epididymal weight. The daily production of spermatozoa per animal in the breeding season was estimated to be 4.0-6.4 x 10(9).
Ovulation and LH secretion in the goat after intravaginal progestagen sponge-PMSG treatment.
The time and rate of ovulation, and preovulatory release of LH were examined in Angora goats after treatment with progestagen-impregnated intravaginal sponges with or without PMSG during the anoestrous and breeding seasons. The administration of PMSG was necessary to stimulate a satisfactory ovulatory response in lactating and non-lactating females, and in the anoestrous and breeding seasons. Increasing doses of PMSG increased the ovulation rate in non-lactating goats and advanced the time of ovulation in lactating and non-lactating females. The advancement of time of ovulation and of the preovulatory LH surge was most pronounced when PMSG was administered 48 h before rather than at sponge removal.
Effects of seminal plasma and of its removal and of egg yolk in the diluent on the survival of fresh and frozen-thawed spermatozoa of the Angora goat.
After twofold dilution with Tris-glucose medium, buck seminal plasma was a poorer milieu than ram seminal plasma for the maintenance of viability of freshly collected spermatozoa of both species. Egg yolk (9% v/v) in the diluted buck seminal plasma caused coagulation of the medium coupled with death of spermatozoa after 2 h incubation at 37 degrees C. Removal of seminal plasma by centrifugation (washing) of buck semen was beneficial for the survival of spermatozoa after freeze-thawing, but the effect depended on the intensity of washing. When the semen was diluted 6- or 11-fold, double washing was more effective than single washing. However, the efficiency of the latter method, after 21-fold dilution, was similar to that of double washing at 11-fold extension. Survival of washed spermatozoa was better when the resuspending-freezing medium contained 1.5-12% (v/v) egg yolk than no egg yolk. Egg yolk concentration higher than 1.5% (v/v) depressed the post-thawing survival of non-washed spermatozoa.
Deep freezing of Angora goat semen: effects of diluent composition and method and rate of dilution on survival of spermatozoa.
Five factorial experiments were conducted to examine the effects of concentration of tris(hydroxymethyl)aminomethane (Tris), type and concentration of sugar in the diluent, rate and method of dilution on the survival of goat spermatozoa after freezing by the pellet method. Spermatozoa tolerated a relatively wide range in concentration of Tris, but the cell survival depended on the type of sugar included in the Tris diluent. Glucose and fructose were more suitable components than lactose or raffinose. Survival of spermatozoa after thawing was better for three- to fivefold prefreezing dilution. There was interaction between method of semen dilution (one-step, two-step), holding time at 5 degrees C, and glycerol concentration. The best result was obtained after one-step dilution at 30 degrees C (Tris 375 mM-glucose 41.625 mM-citric acid 124 mM), 1.5 h holding at 5 degrees C, and with 4% (v/v) glycerol concentration in the diluted semen.
Fertility of frozen-thawed boar semen.
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Examination of some processing methods for freezing boar semen.
Five experiments were conducted to examine the effect of processing methods and diluents on survival and morphology of boar spermatozoa after freezing. Post-thawing survival of spermatozoa was better for Beltsville-F3 (BF3) than for tris-fructose-EDTA freezing diluent when the seminal plasma and glycerol were removed prior to freezing (method A). Both freezing diluents yielded similar viability results when the spermatozoa were frozen in the presence of siminal plasma and glycerol (method B). Viability of spermatozoa after thawing was better when glycerol concentration in the prefreezing diluent (method A) or in the freezing medium (method B) was 2-5 and 5-0 rather than 7-5%. Cooling of diluted semen to 5 degrees C beyond 4 h decreased the post-thawing survival of spermatozoa. The proportion of spermatozoa with undamaged acrosomes after processing and thawing by different methods was indistinguishable and relatively low. When the semen was frozen at cell concentrations ranging from 0-25 to 2-0 X 10(9)/ml, the viability of spermatozoa declined with increasing concentration following freezing in BF3, and S-1 diluents. Viability results were very similar for all cell concentrations examined when tris-fructose-EDTA diluent was used, indicating the possibility of freezing boar semen in a concentrated state.
Fertility test of frozen boar semen.
The fertility results of two experiments are presented. In experiment 1, the semen was frozen in tris-fructose-EDTA or BF3 diluents at 0-25 X 10(9)/ml sperm concentration and extended after thawing with either seminal plasma (SP) or the freezing medium (FM) containing no cryoprotective agent. In the second experiment the semen was glycerolated by two methods, frozen at 1-0 X 10(9)/ml sperm concentration, and extended wtih FM before insemination. Fertility after double insemination within one oestrus with semen frozen in tris-fructose-EDTA or BF3 diluents varied depending on the medium used for extension of thawed semen. The farrowing rates for semen frozen in the former diluent with FM and SP post-thawing media were 4/8 and 1/8 respectively, and for semen frozen BF3 diluent with FM and SP post-thawing extenders 1/8 and 5/8. The mean farrowing for the 32 animals inseminasted was 34-4%. Pregnancies for semen frozen in tris-fructose-EDTA and glycerolated at 30 or 5 degrees C were 5/12 and 4/12 respectively, and for single and double inseminations 6/12 and 3/12 respectively. Of 24 animals inseminated 37-5% farrowed.
Proceedings: Fertility of boar semen frozen-stored for 2 1/2 years.
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Proceedings: effect of alpha-chlorohydrin on the fertility of rams and on the metabolism of spermatozoa in vitro.
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Fertility following inseminations with frozen-thawed reconcentrated and unconcentrated ram semen.
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Effect of composition of tris-based diluent on survival of boar spermatozoa following deep freezing.
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Fertility after surgical insemination with frozen boar semen.
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Proceedings: Fertility after surgical insemination with frozen boar semen.
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