[Current problems in the research on active substances. 13. Fluidity of cell membranes and mobility of cellular receptors, immunological and pharmacological aspects].
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Biomedical subjects
Publications and source records attributed to S Schnitzler.
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The guinea pig alveolar surfactant reduces the contraction of the isolated guinea pig ileum, induced by Angiotensin II. It is tentatively proposed, that the surfactant system suppresses the reactivity of lung blood vessels towards contacting stimuli.
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The carbohydrate binding glycoprotein Anti-AHP binds to the plasma membrane of the (transformed) L-cell both in agglutination reactions and by the immunofluorescence technique. On the surface of (normal) mouse embryo fibroblast cells Anti-AHP receptors were not detectable. Anti-AHP is suitable as another marker for L cells. The random distribution of the binding sites at 4 degrees C changes with elevated temperatures (clustering, capping of sites). Anti-AHP inhibits the growth of L cells much more than that of fibroblasts. It does not act as a mitogen for the investigated cells.
Antisera produced in rabbits against Triton X-100 solubilized human erythrocyte ghosts formed several several precipitation lines in immunoelectrophoresis and immunodiffusion tests against the solubilized ghosts. The antisera were used for detecting several membrane components during chromatographic separation. A membrane component was partially isolated using gel filtration on SEPHADEX G-200 (fraction F1/F2). Spectrin (Tektin A) was shown to be the main moiety of these fractions. Associated proteins (haemoglobin, GAPD) could not be detected by the antisera against whole ghost solubilisate.
Using polyvalent antisera directed against solubilized human erythrocyte ghosts in the immunoelectrophoresis we obtained some precipitation arcs. Antisera against the purified spectrin allowed the identification of spectrin in the crossed immunoelectrophoresis.
The cyst fluid of cestodes from various animals kept in zoological gardens was examined for the presence of A-, B-, H- and P1-blood group activity. While the results in the ABH-system were negative, the cysts from 2 of 13 animals examined (1 elk and 1 reindeer) presented P1-blood group activity.
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