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S Schoenbeck

Publications and source records attributed to S Schoenbeck.

7 recordsLinked to original sources
PubMed1991

Detection of individual Peyer's patch T cells that produce interleukin-5 and interferon-gamma.

A method was developed to detect individual interferon-gamma (IFN-gamma)- and interleukin-5 (IL-5)-producing cells among freshly isolated T cell populations and long term lines of CD4+ Peyer's patch T cells using frozen semi-thin sections of paraformaldehyde fixed-T cells and immunofluorescence techniques. Using this method, individual CD4+ Peyer's patch T cells could be shown to produce IFN-gamma, characteristic of the T helper 1 (Th1) T cell type, IL-5, characteristic of the T helper 2 (Th2) T cell type, as well as both IFN-gamma and IL-5. These data support the notion that Th1 and Th2 cells derive from a common T cell precursor.

Animals↗
PubMed1989

Vicia villosa agglutinin separates freshly isolated Peyer's Patch T cells into interleukin 5- or interleukin 2-producing subsets.

Murine L3T4 T cells freshly isolated from Peyer's Patch were fractionated based on differential adherence to Vicia villosa agglutinin (VVA). VVA adherent cells secreted IL-5, but not IL-2, after stimulation with Con A and IL-1. In striking contrast, VVA nonadherent PP L3T4 T cells secreted IL-2, but not IL-5, under the same conditions. In addition, supernatants from VVA adherent, but not from VVA nonadherent cells cultures, enhanced IgA secretion by LPS-stimulated splenic B cells to the same extent as purified IL-5. Thus, IL-5-producing T cells are present in PP in situ and may play an important role in the development of mucosal immunity. Further, differential adherence to VVA can be used to separate T cell populations that preferentially secrete IL-5 or IL-2.

Animals↗
PubMed1989

Interleukin 5 is a differentiation factor for IgA B cells.

Interleukin 5 (IL5) stimulates murine B cells to IgA production in vitro. The present study examined the B cell target of IL5 activity, and asked whether IL5 acts as an isotype switch factor, a growth factor, or a differentiation factor on B cells that produce the IgA isotype. Lipopolysaccharide-activated surface IgM-positive, surface IgA-positive or surface IgA-negative B cell populations from spleen or Peyer's patches were stimulated with IL5, after which the expression of cell surface IgA, IgA secretion, and numbers of IgA-secreting cells were determined. IL5 increased the IgA response by acting on B cells that are already committed to IgA expression. Thus, with regard to the IgA response, IL5 acts as a differentiation factor, rather than a growth or IgA isotype switch factor.

Animals↗