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Biomedical subjects

S Schor

Publications and source records attributed to S Schor.

At least 19 recordsLinked to original sources

Quantitative study of various factors influencing the migration of lymphocytes in vitro: glucocortico-steroid, PHA, cyclosporin A and heparin.

Various agents known to influence the lymphocyte recirculation in vivo were investigated for their effect on the migration of lymphocytes in 3-D collagen gel. Both glucocortico-steroid and cyclosporin A were shown to inhibit the migration in a dose dependent manner. In contrast heparin and PHA treated lymphocytes migrated at a significantly faster rate.

Cell Movement

Migration of leukaemic lymphocytes (chronic lymphocytic leukaemia) through a 3-D collagen gel: a possible prognostic factor.

Migratory properties of malignant lymphocytes in 27 patients with chronic lymphocytic leukaemia (CLL) were investigated using 3-D collagen gel. There was significant impairment of migration in malignant lymphocytes as compared with normal lymphocytes. Moreover there was an inverse correlation between Rai's staging and the migration index. Patients with progression of disease also showed a decrease in migration index. There was no correlation between kappa and lambda type in terms of migration index. The prognostic significance of the migratory properties of malignant lymphocytes is also discussed.

B-Lymphocytes

Quantitative study of the migration of lymphocyte subsets through 3-D collagen gels.

Migratory properties of normal peripheral blood lymphocyte subsets were studied with a 3-D collagen gel model. Both the T and B subsets entered the gel at the same rate, but once inside the T subsets migrated significantly faster than the B subsets. In the T subsets, both the T-helper and T-suppressor cells entered the gel at the same rate, but the former migrated significantly faster once inside the gel. The biological significance of their migrations is discussed.

B-Lymphocytes

Susceptibility of skin fibroblasts from individuals genetically predisposed to cancer, to transformation by the tumour promoter 12-O-tetradecanoylphorbol-13-acetate.

Skin fibroblasts from patients with hereditary retinoblastoma (RB) and familial polyposis coli (FPC) were chosen for study since their predisposition to the tumour may be due to an inherited "initiation" event which is present in every cell. Thus, it might be predicted that skin fibroblasts from these patients would exhibit increased susceptibility to in vitro transformation by tumour promoters alone. In the case of skin fibroblasts from RB patients, transformation as assessed by the ability of the cells to grow in semi-solid medium and their migration in collagen gels did not occur. However, experiments involving skin fibroblasts from FPC patients showed certain of these cells to grow in semi-solid medium following treatment with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) alone, although the pattern of migration of the parent cell population in collagen gels was unchanged and they were non-tumorigenic in nude mice. The clones which grew in semi-solid medium, although stable with regard to anchor-age-independent growth, were also unaltered in terms of their migration pattern in collagen gels and their tumorigenicity in nude mice, and were considered not to be completely transformed. Parallel cytogenetic analysis showed that, during the course of these transformation studies, TPA significantly increased not only tetraploidy but also the chromosome aberration frequency. Several quadriradial figures were noted.

Adult

Collagen gels as a matrix for haemopoiesis.

We have shown that collagen gel can be used as a culture matrix for the cloning of granulocyte/macrophage progenitor cells (CFU-C), the production of foci of marrow stromal cells and the maintenance of stem cell proliferation, differentiation and the production of CFU-C. Since collagen is a physiological matrix and allows the simultaneous growth of a variety of cellular elements, the system should prove useful for examining the role of cell/cell interactions and regulatory molecules involved in haemopoiesis.

Animals

Evaluation of electrocardiogram in emergency room as a decision-making tool.

The contribution of the electrocardiogram to the clinical judgment used by the physician in the emergency room to determine the necessity for hospitalizing patients was evaluated. Thirty-five percent of all 1,578 patients with presumed myocardial infarction referred to the Chaim Sheba Medical Center, Tel Hashomer, Israel, for a one-year period had subsequently diagnosed myocardial infarctions. The ECG in the emergency room detected only 65 percent of these. The physician's clinical judgment was impressive in his decision to admit to the hospital almost all of the remaining 35 percent, while not admitting very many of the patients who did not have subsequently diagnosed myocardial infarctions. When the myocardial infarction was not evident on the ECG and the abnormalities on the tracings were identical for patients with subsequent myocardial infarctions and those without, again the physician made the right choice more often than the wrong. The follow-up ECG also attested to the good judgment of the physician in the emergency room. Of the emergency room ECGs of patients without subsequent myocardial infarctions who were admitted to the hospital, 17 percent showed myocardial infarction by follow-up, while this happened to only 2 percent of those denied admission.

Decision Making

Disposition of presumed coronary patients from an emergency room. A follow-up study.

All patients with presumed coronary problems seen at the Chaim Sheba Medical Center during a one-year period were followed up. The fate of those who were not hospitalized and the factors contributing to the two types of erroneous decisions, ie, refusing hospitalization to those needing it and unnecessary hospitalization of others, were evaluated. Approximately 50% of the patients were not admitted. Myocardial infarctions were later diagnosed in 6% of these patients. Another 8% were eventually categorized as other cardiac emergencies. Ten percent of all patients subsequently diagnosed as having myocardial infarctions were not admitted. On the other hand, 56% of the patients whose cases were later not considered to have been emergencies were hospitalized unnecessarily. Previous hospitalization for cardiac disease played a major role in making an error of both types. Other factors influencing the physician's decision regarding the patients' disposition included their age, sex, ethnic origin, and the findings from the emergency room electrocardiogram.

Adult

Acute myocardial infarction. Prognostic value of white blood cell count and blood glucose level.

Data obtained in the course of a nationwide study of patients with first acute myocardial infarction (AMI) demonstrated substantially higher hospital mortality among patients with leukocytosis or elevated blood glucose levels, or both. It is suggested that the combination of these two measurements could be used as a handy diagnostic indicator in the evaluation of some AMI patients and as a criterion for the patients' immediate disposition to a coronary care unit, rather than to a general ward.

Acute Disease

Reduction of hospital mortality from acute myocardial infarction by anticoagulant therapy.

A nationwide study of all 2330 patients hospitalized for a first definite acute myocardial infarction in Israel during a one-year period revealed a significantly lower 21-day mortality rate of patients treated by anticoagulants: 8.3 versus 27.3 per cent in those not receiving such therapy (p less than 0.0001). Out of the 22 hospitals studied, the effect was present in 18, absent in two, and not assessable in two. There was a highly significant inverse correlation between the proportion of patients receiving anticoagulant therapy in each hospital and the total 21-day mortality from infarction in that institution (r equals 0.57; less than 0.01). The superior survival of patients on anticoagulant therapy did not seem related to differences in age, sex, disease severity, site of infarction, diagnostic criteria or type of hospital. Despite the obvious limitations of retrospective studies the data justify a re-evaluation of the approach to the problem by further investigative effort.

Acute Disease

Some epidemiologic aspects of acute myocardial infarction in Israel.

A nationwide study of acute myocardial infarction in Israel was carried out during a one-year period. There were 2,504 new and 968 repeated attacks. Annual incidence rate of first attacks was 0.9 per 1,000 (1.3 in men and 0.5 in women). Incidence was highest among the European born in both sexes. But, while the pattern of the native born men was similar to that of those born in Europe, it approached the incidence of the Asian-African born in women. The sex ratio was relatively higher in those ethnic groups where male incidence was relatively higher and vice versa. Length of residence in the country had little effect on the incidence rates.

Acute Disease

Factors affecting immediate mortality of patients with acute myocardial infarction: a nationwide study.

The hospital mortality was evaluated for 21 days in all patients with a first attack of acute myocardial infarction (AMI) in Israel. Total mortality was 21.1 percent. The following factors were associated with relatively better prognoses: age, young; sex, male; marital status, married; ethnic origin, European; site, subendocardial; history of smoking, and, to a certain degree, hypertension; low levels of white blood cells, blood sugar, serum transaminase and cholesterol; and anticoagulant therapy.

Acute Disease