PubMed Health⌕ Search

Biomedical subjects

S Shiobara

Publications and source records attributed to S Shiobara.

At least 91 records · Page 5Linked to original sources
PubMed1984

T lymphocyte reconstitution in long-term survivors after allogeneic and autologous marrow transplantation.

T cell subsets and their immune reactivities were studied in long-term survivors after bone marrow transplantation and the results of autotransplanted and allotransplanted patients were compared. These two groups of patients (4 autotransplants and 4 allotransplants) were roughly comparable in terms of their underlying diseases, pretransplant conditioning regimens, supportive care, and posttransplant sampling days for immunological studies. Significant differences were observed between autologous and allogeneic marrow recipients in the total number of OKT3-, OKT4-, OKT8-, and OKIa1 -positive cells. Similar differences were observed between transplant patients and normal controls. Decreased OKT4 cells and increased OKT8 cells resulted in inversion of the OKT4:OKT8 ratio, which was significantly lower in allotransplanted compared with autotransplanted patients, and both groups of transplant patients showed depressed responses in comparison with normal controls. In contrast, there were no significant differences in MLR reactivities between transplanted patients and normal controls. When mitogenic responses were analyzed in relation to T cell subsets, phytohemagglutinin responsiveness showed a significant correlation with OKT4:OKT8 ratios (P less than 0.01) and the proportions of cultured OKT4 cells (P less than 0.01). These observations suggest that T lymphocyte reconstitution is still incomplete or abnormal in long-term survivors regardless of the type of graft. Furthermore, abnormalities observed in these long-term survivors were characterized by an imbalance of T cell subsets that was more profound in allotransplanted than in autotransplanted patients.

Adolescent↗
PubMed1984

Immunoglobulin synthesis after HLA-identical marrow grafting. V. The role of peripheral blood monocytes in the regulation of in vitro immunoglobulin secretion stimulated by pokeweed mitogen.

The ability of purified monocytes to regulate in vitro immunoglobulin (Ig) production was examined in 12 patients after HLA-identical marrow grafting. Five patients were studied less than 3 mo after grafting and seven more than 1 yr after grafting. One of the former had acute graft-vs-host disease and five of the latter had chronic graft-vs-host disease. Ficoll-Hypaque-separated peripheral blood mononuclear cells from patients, normal marrow donors, or healthy unrelated individuals were separated into T and non-T cells by sheep erythrocyte rosetting. Highly enriched monocyte and B cell subpopulations were obtained by placing the non-T cells over discontinuous Percoll gradients. Co-cultures of patient or normal monocyte populations with either normal or patient T and B cells with pokeweed mitogen were performed. A hemolytic plaque assay was used to assess Ig secretion after 6 days of culture. Co-culture of T and non-T cells from 10 of 12 patients failed to produce Ig. Monocyte-enriched fractions from all patients provided normal accessory cell functions when co-cultured with normal T and B cells. Two of five patients with chronic graft-vs-host disease had monocytes that suppressed Ig synthesis at high ratios of monocytes to normal T and B cells. Normal monocyte-enriched fractions did not restore Ig production to T and B cells of patients whose T and non-T cells failed to produce Ig. These data indicate that the observed defects in pokeweed mitogen-driven Ig secretion after marrow grafting are due primarily to defective T and B cell functions and that the monocyte accessory function is intact in most patients studied.

Adolescent↗
PubMed1983

[Autologous bone marrow transplantation in the treatment of nasopharyngeal carcinoma--report of 2 cases].

Two patients with nasopharyngeal carcinoma (NPC) were treated with "marrow-lethal" chemoradiotherapy and infusion of cryopreserved autologous marrow. Patient AUT 04 (unique patient number), a 28-year-old male, was referred to us because of the right cervical lymphadenopathy. He was diagnosed NPC at stage II (T1N2M0). A trial of combined-modality therapy using autologous bone marrow transplantation (auto-BMT) was scheduled for possible cure. Before auto-BMT, he was treated with a conventional dose of radiation to the nasopharyngeal and bilateral cervical areas. For the pretransplant marrow-lethal therapy, he received high-dose cyclophosphamide (CY), 120mg/kg, and 1,000 rad total body irradiation (TBI). Within 24 hr after TBI, previously cryopreserved-thawed autologous bone marrow cells (1.37 X 10(8)/kg) was infused to the patient. Rapid and complete hematologic recovery was observed and he is now alive in a disease-free remission state 28 months after transplantation. Patient AUT 08, a 18-year-old female, was referred to us for the treatment of head and neck tumor with auto-BMT. She was diagnosed NPC at stage IV (T4N2M0). After cryopreservation of bone marrow, she was treated with local radiation and doxorubicin (80mg X 2d). Then she was conditioned with high-dose CY and upper half body irradiation and received auto-BMT (marrow dose: 1.2 X 10(7)/kg). Successful engraftment was obtained with tumor response but she developed recurrence of the disease 12 months after transplantation. Although the data are too preliminary, auto-BMT is evaluated as one of the favorable treatment approaches for some selected patients with NPC.

Adolescent↗
PubMed1983

Hematologic recovery following autologous and allogeneic bone marrow transplantation.

Posttransplant hematologic recovery was compared between 9 autologous and 11 allogeneic marrow transplant patients who received similar marrow-lethal chemoradiotherapies before transplantation. Although the autotransplant patients were infused with much lower marrow doses compared with the allotransplant patients, they showed adequate hematologic recovery with acceptable risk. Regardless of marrow doses, delayed platelet recovery and failure of platelet recovery were observed in 7 patients. All of these patients experienced early transplantation-related complications before engraftment. Despite the limited number of patients, a number of myeloid progenitor cells (CFUC) infused correlated significantly with the period for recovery of polymorphonuclear cells in autologous marrow recipients while there was no significant correlation found between marrow dose and hematologic recovery in both groups of patients. Furthermore, autotransplant patients showed a characteristic recovery pattern of peripheral blood lymphocytes in an early posttransplant period, whic was not observed in allotransplant patients.

Acute Disease↗