Biomedical subjects
Publications and source records attributed to S Slovin'ski.
Basing on measurements of the activities of 7 lysosomal enzymes, the authors have assessed the enzymic activities of neutrophils in 22 patients with protracted renal insufficiency treated by hemodialysis. Induction of some examined enzymes was observed, which remained virtually unchanged after hemoperfusion.
B-lymphocyte counts, immunoglobulin levels, blood serum antiA and antiB isoagglutinin concentrations and lysozyme activity, and hemolytic activity of the complement were measured in 85 healthy men aged 18 to 42 (mean age 34) smoking 15-25 cigarettes daily for 2-25 years and in a reference group of 49 nonsmokers aged 17 to 50 (mean age 31). Tobacco smoking was found to have no effect on the peripheral blood B lymphocyte count, nor on the blood serum concentrations of group isoagglutinins. In men smoking for 6.5 years on an average the absolute count of lymphocytes was elevated vs. the reference value. In those smoking for 18.5 years on an average IgA, IgG, and lysozyme concentrations were lowered. These results evidence that the mechanism of humoral immunity weakening in tobacco smokers is intricate and related to a deficit of many proteins responsible for immunobiological processes in human body.
T-lymphocyte counts have been measured and the skin test with amnestic antigens performed in 85 clinically healthy men aged 18-42 (the mean age 34) smoking 15-25 cigarettes daily during 2-25 years and in a reference group of 49 non-smokers aged 17-50 (the mean age 31). The absolute count of circulating lymphocytes has been found increased in 38 subjects who smoked for up to 10 years (the mean period 6.5 years), whereas in the subjects who smoked for more than 10 years (the mean period 18.5 years) the absolute and the relative counts of T-lymphocytes have been decreased, the tuberculin test has been much more often negative (14.9% vs. 6.1 and 5.3%), as well as the distreptase test (19.1% vs. 8.2 and 5.3%). These data confirm the suppressor effect of tobacco smoke, inhaled for a long time, on cell-mediated immunity in humans.
Neutrophil level in blood (1 l) and those of certain enzymes in neutrophils were measured in 36 patients with stomach tumors and 40 healthy controls. The range of the enzymes included myeloperoxidase (MPO), beta-glucuronidase (GR), N-acetyl-beta-D-glucosaminidase (NAG), acid phosphatase (AcP), alkaline phosphatase (AlP), lactate dehydrogenase (LD) and dehydrogenase-glucoso-6-phosphoric acid (DG-6-P). Glycogen and lipids were also assayed. The MPO and AcP levels of neutrophils in cancer patients were found to be significantly higher than in healthy controls, whereas the levels of the other enzymes were not. The glycogen and lipid concentrations in neutrophils in cancer patients were significantly lower than in healthy controls.
Cytochemical assays of neutrophil acid phosphatase, beta-glucuronidase, N-acetyl-beta-D-glycosamidase (NAG), myeloperoxidase activities, and the Sudan black B test, were carried out in 25 patients with myocardial infarction. Leucocytosis seen in the early days of infarction was associated with increased proportion of neutrophils characterized by high activities of the enzymes in question and enhanced reaction with Sudan black B. Neutrophilosis of the early myocardial infarction may result from the activity of the marginal cell fraction. The fact that increased myeloperoxidase activity and the enhanced response to Sudan black B persist through 14 days of the disease, coupled with a rise in neutrophil NAG activity on day 14, expands possibilities of myocardial infarction diagnosis.
Explore the source record for details and available documents.
Explore the source record for details and available documents.