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Biomedical subjects

S Sonis

Publications and source records attributed to S Sonis.

6 recordsLinked to original sources
PubMed1978

Circulating immune complexes in patients following clinically curative resection of colorectal cancer.

Sixty-nine patients have been followed prospectively after curative resection of Dukes-Kirklin B-2 or C colorectal cancer. Serial plasma samples were studied in selected patients to determine changes in circulating immune complex concentrations (CIC) following primary tumor resection, and to compare serial plasma CIC and carcinoembryonic antigen (CEA) levels. CIC was determined in an average of seven serial samples per patient by inhibition of antibody-dependent cell-mediated cytotoxicity (ADCC). CEA assays were performed by the Hanson Z-gel method. Two distinct patterns of serial CIC have emerged. In seven patients with no known tumor recurrences, serial CEA levels and CIC oscillated regularly and were inversely related. In seven of eight patients whose tumors recurred, both CEA and CIC rose together. In three patients with elevated plasma CEA levels due to inflammatory bowel disease, serial Ag-Ab complex concentrations did not vary, nor did separated Ag or Ab fractions inhibit ADCC. These data suggest that, in patients following curative resection of colorectal cancer, serial changes in circulating immune complexes may discriminate between transient CEA elevations which occur despite no known tumor recurrence and tumor recurrence which is beyond the capacity of adequate host antitumor defense.

Adenocarcinoma↗
PubMed1977

Assessment of drug sensitivity of human leukaemic myeloblasts. I. Labelling human myeloblasts with 125IUdR for survival studies in mice.

The compound (125)IUdR can be incorporated in a stable form into the DNA of cells. The isotope is released if labelled cells or their progeny die. Consequently the rate of (125)I excretion from mice can be used to follow the fate of labelled cells in vivo. Using these principles we show:(1) Sufficient label can be incorporated in vitro into both fresh and cryopreserved human leukaemic myeloblasts, in non-toxic concentrations, to allow their survival in mice to be estimated by whole-body counting;(2) The release of isotope from labelled cells is sufficiently slow to offer reasonable expectation that this technique can be used for assessing the sensitivity of myeloblasts to cytotoxic agents in vivo (an application described in the second paper in this series, Sonis, Falcão and MacLennon, 1977);(3) The rate of (125)Iexcretion from mice injected with myeloblasts from different donors varies. This probably reflects different rates of spontaneous death of injected myeloblasts;(4) Active rejection of myeloblasts starts within 48 h of their injection into mice;(5) Indirect evidence that phagocytic cells may be active agents in myeloblast destruction in mice;(6) Various methods of immunologically depriving mice were assessed to see if they would result in a useful increase in survival of injected human myeloblasts. We conclude that there is little advantage and some limitations in using mice thus deprived;(7) One of the agents used for immunological deprivation-silica powder-markedly decreased the rate of (125)I loss from mice injected with labelled killed myeloblasts. This experience emphasizes the importance of including the killed-cell control in this assay.

Animals↗
PubMed1975

Cyanoacrylate as an adhesive for other medicaments: a study with triamcinalone on the healing of experimental oral ulcers.

This study was undertaken to determine whether cyanoacrylate can be used to adhere other medicaments to oral ulcers in the hope of promoting healing. Triamcinalone was chosen since it is used presently in pastes in the oral cavity. Sixty rats were dived into three equal groups. In each animal under anesthesia with ether, bilateral equivalent ulcers, 2 mm in depth and diameter, were made with the tip of a rongeur on the midlateral aspect of the tongue. The right side served as a control. The left side was experimental and received the following: group I--isobutyl-2-cyanoacrylate only; group II--powdered triamcinalone acetonide; group III--triamcinalone covered with cyanoacrylate. All materials were reapplied every 24 hours for 4 days. Two animals from each group (6) were sacrificed at 1, 4, and 8 hours and 1, 2, 3, 5, 14, and 21 days. The results indicate that a powdered medicament can be held in place on oral ulcers for at least 24 hours. The ulcers with cyanoacrylate revealed less edema and cellular response for up to 24 hours. Healing of the ulcers was neither promoted nor delayed in any group.

Animals↗