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Biomedical subjects

S Spisani

Publications and source records attributed to S Spisani.

At least 109 records · Page 6Linked to original sources
PubMed1984

[Indomethacin and oxamethacin as modulators of the production of superoxide anion in human leukocytes].

Treatment of human neutrophils with the two chemoattractants formyl-methyonyl-leucyl-phenylalanine and opsonized zymosan or with phorbol myristate acetate induce the production of superoxide anion (O-.2), which plays a role in the inflammatory reaction and microbicidal activity. The time-course of O-.2 release and the extent to which the anion is produced are related to the nature of the stimulating agents. Anti-inflammatory non steroid drugs, such as indomethacin and oxamethacin, exert a variable depression of the oxidative burst, which does not seem correlated to the inhibition of prostaglandin synthesis, but to the lipophilic character of the drug molecule.

Humans↗
PubMed1984

[An analog of formyl-met-leu-phe effects the movement of human leukocytes].

Two tripeptides, related to the chemotactic formyl-peptide, are tested for its ability to affect random and directional locomotion and to induce chemotaxis of polymorphonuclear leukocytes. The results indicate that the methyl ester of formyl-methyonyl-leucyl-phenylalanine possesses a biological activity towards human phagocytes, including a chemotactic potency, comparable to that of unmodified peptide. Therefore, the free carboxyl group does not seem essential to generate active leukoattractant. On the contrary, the replacement of the formyl group by the butyloxycarbonyl results in a drastic loss of biological activity. Our data may indicate that the two formyl-peptides interact with the same binding site on the cell membrane.

Binding Sites↗
PubMed1984

Effect of antiinflammatory agents on neutrophil superoxide production in rheumatoid arthritis.

Granulocyte superoxide production by different stimuli was studied in 14 patients suffering from rheumatoid arthritis, and in four cases defective O(2) generation was shown. The effect of two chemically related drugs, such as indomethacin and oxamethacin, was also evaluated, since we have previously investigated the action of antiinflammatory agents on cell locomotion. Indomethacin did not affect O(2) production, whereas oxamethacin reduced significantly superoxide generation in PMNs from all subjects tested. Moreover, the extent of the effect was dependent on the stimulant used, being larger when the activation of O(2) generating system was induced by opsonized zymosan.

Anti-Inflammatory Agents↗
PubMed1984

Effect of anti-inflammatory drugs on leukocyte superoxide production by soluble and particulate stimuli.

When polymorphonuclear leukocytes are treated with soluble and particulate substances the cells produce superoxide anion O2-. which plays a role in the activation of the oxidative metabolism. The time-course of O2-. release and the extent to which the anion is produced are related to the nature of the stimulating agents. Anti-inflammatory non steroid drugs, such as indomethacin and oxamethacin, exert a variable depression of the oxidative burst, which does not seem correlated to the inhibition of prostaglandin synthesis.

Anti-Inflammatory Agents↗
PubMed1984

Chemotactic behaviour of peripheral and synovial neutrophils during rifamycin SV therapy in rheumatoid arthritis.

The effect exerted by rifamycin SV, used intra-articularly in the treatment of rheumatoid arthritis, on polymorph function was studied. Random and directional locomotion of synovial fluid neutrophils was compared with that of peripheral blood cells in 10 patients followed up during 5 drug applications. PMNs from the two sources were characterized by different responsiveness to this pharmacological agent: blood cells activated chemotaxis in a dose-response way during rifamycin therapy, whereas synovial polymorphs did not modify their locomotor behaviour. It is proposed that the presence of immune complexes and/or factors produced by cell-cell interactions in the articular space may change the synovial neutrophil response to stimuli.

Adolescent↗
PubMed1982

[Regulation of the functions of human leukocytes by oxametacine].

Non steroidal anti-inflammatory drugs, such as oxametacine, are generally used in treatment of rheumatoid disease. In an 'in vitro' experimental model, the drug efficacy was tested on leukocyte functions. Locomotion, both random and directional, phagocytic activity and superoxide production of normal and rheumatoid PMNL were tested in the presence of varying concentrations of oxometacine. Locomotion was evaluated by using modified Boyden chambers; phagocytosis was tested by number of yeast particles injested and by NBT reduction; superoxide production was assayed by reduction of ferricytochrome C. In our conditions the drug exhibited a strong anti-inflammatory effect. In fact, chemotaxis and anion production were specifically depressed in a dose-dependent way.

Arthritis, Rheumatoid↗
PubMed1982

[Medium molecular weight uremic toxins and endogenous polyamines. Behavior of polymorphonuclear leukocyte chemotaxis with respect to chromatographic peaks of dialysate and standard polyamines].

The aim of our study is to evaluate the eventual activity of the total dialysate of two uremic nephrectomized patients in recirculating dialysis and the chromatographic peak of the dialysate fractionated by Sephadex column G 15 on PMN chemotaxis. Only the total dialysate and the chromatographic peak B showed inhibition of chemotaxis. On the contrary the commercial polyamines in the same concentration range, and the other chromatographic peaks, containing polyamines too, did not revealed inhibition. Our data show, therefore, that the chemotaxis inhibition could be due to the middle-molecules present in the peak B, rather than the polyamines itself. Polyamines were determined by dansylation method, separated by thin layer chromatography and quantified by spectrofluorimeter. Chemotaxis was evaluated using the modified Boyden chamber.

Chemotaxis, Leukocyte↗
PubMed1982

Synthetic prostaglandin1 analogue: in vitro studies on human neutrophils.

Prostaglandin (PG) E1 and chemically related compounds 15 alpha-11-deoxy-8-azaPGE1 and 15 epi-11-deoxy-8-azaPGE1 were examined in vitro for their capacity to modulate some physiological functions of human polymorphonuclear leukocytes (PMN). PGE1 was completely devoid of chemotactic activity and did not affect leukocyte phagocytosis. On the contrary, 15 alpha-11-deoxy-8-azaPGE1 showed a potent leukotactic activity. In addition, this synthetic substance significantly depressed the phagocytic activity and the concomitant NBT reduction by leukocytes. The analogue 15 epi-11-deoxy-8-azaPGE1 did not exert any of these effects. From these results it appeared that the substitution of the carbon-8 by the nitrogen and the lack of hydroxyl group at the position 11 into the PGE1 structure, make the compound active towards human neutrophils.

Alprostadil↗
PubMed1982

The effect of rifamycin SV on neutrophil functions in patients with rheumatoid arthritis.

The chemotaxis, phagocytic capacity and reducing activity of neutrophils derived from peripheral blood of patients with rheumatoid arthritis (RA) did not differ from those of control. However, some significant differences between neutrophils from rheumatic and healthy subjects emerged in the presence of rifamycin SV. The chemotactic response of neutrophils from patients with RA was activated by rifamycin SV, whereas cells from controls did not orient their locomotion towards the drug. Moreover, incubation of RA patient's cells with rifamycin SV in vitro depressed phagocytic and reducing activities; the same treatment on normal cells failed to alter these functions. A correlation between improvement of clinical symptoms after treatment of RA by local infiltration with rifamycin SV, observed by others, and the impairment of phagocytosis and NBT reduction, here described, was suggested.

Arthritis, Rheumatoid↗
PubMed1982

Defective responsiveness to natural and pharmacological molecules of neutrophil locomotion in rheumatoid arthritis disease.

Neutrophils derived from peripheral blood of patients with rheumatoid arthritis (RA) exhibited a defective responsiveness to natural mediators of inflammation, namely histamine and serotonin, and to the anti-inflammatory drugs ibuprofen and naproxen, in spite of the fact that the basic status of motility was normal. Not even pretreatment of granulocytes with substances restored the capacity to modulate the random and directional locomotion. This neutrophil functional defect was correlated with an anomalous response to rifamycin SV, previously observed in rheumatic states.

Arthritis, Rheumatoid↗
PubMed1981

[Relationship between structure and function of a chemotactic factor for human leukocytes].

Casein, a phosphoprotein forming aggregates in solution, exerts chemotactic activity for human neutrophils. The protein was filtered on Sephadex G 100 to obtain fractions of homogeneous mol. weight and the column fractions were tested for chemotactic activity. The chemotactic activity was found only in the lighter peak, which was then digested by trypsin. The digested material was purified by filtration through AcA-54 and the 6.000 M.W. peptide containing the most phosphate groups and tyrosines resulted chemotactic for PMN. Most likely the phosphate groups are important in chemotactic recognition.

Caseins↗
PubMed1981

[Effect of rifamycin SV on neutrophil functions in patients with rheumatoid arthritis].

The antibiotic rifamycin SV (RSV) has been successfully used by others on the local treatment of rheumatoid arthritis (R.A.). Since polymorphonuclear leukocytes (PMNL) are involved in the synovial inflammatory process we tested the 'in vitro' effect of RSV on PMNL functions, such as locomotion and phagocytosis. PMNL locomotion was evaluated by using modified Boyden Chamber and phagocytosis was tested by the number of yeast particles ingested and by NBT reduction. The, the functions of PMNL derived from 7 R.A. patients in therapy only with non steroidal anti-inflammatory drugs were compared with those of PMNL from 14 patients with non inflammatory disease (osteoporosis and osteoarthrosis) in the same therapy and with neutrophils from healthy subjects. It was demonstrated that PMNL derived from patients with both R.A. and non inflammatory disease activated their directional locomotion towards RSV, on the contrary, cells from healthy subjects were unresponsive. Moreover, only patients with R.A. showed a defective phagocytic capacity and a depression in NBT reducing activity, when PMNL were treated with RSV. This phenomenon might be correlated with beneficial effect observed after local treatment of R.A. with RSV.

Arthritis, Rheumatoid↗
PubMed1981

Leukocyte migration and phagocytosis in progressive systemic sclerosis.

Experiments "in vitro" have been performed to study the chemotactic and phagocytotic response of PMN in 9 patients suffering from progressive systemic sclerosis (PSS). Their granulocytes showed a normal behaviour in all of these functions, when compared with healthy volunteers as controls.

Chemotaxis, Leukocyte↗