Biomedical subjects

S Stair

Publications and source records attributed to S Stair.

3 recordsLinked to original sources

PubMed1990

Hyaluronic acid determinations: optimizing assay parameters.

Assay conditions for determining hyaluronic acid levels in cultured cells have been examined. In cultures labeled with [3H]glucosamine, hyaluronic acid is measured by digestion with a highly specific hyaluronidase from Streptomyces hyaluronlyticus. Products obtained in the presence and absence of preliminary enzyme digestion are precipitated with cetylpyridinium chloride. The precipitation step has been optimized for ion concentration, glycosaminoglycan carrier and for cetylpyridinium chloride levels. Chondroitin sulfate is an effective carrier in the precipitation of radiolabeled product, while unlabeled hyaluronic acid is not. Addition of sulfate to the mixture yields a flocculent precipitate that facilitates subsequent steps of the determination. Optimizing these steps in hyaluronic acid determination can generate two- to three-fold increases in apparent levels of deposition in cultured cells.

Animals↗

PubMedDate not supplied

Compliance with safety glasses wear in monocular children.

Many ophthalmologists prescribe polycarbonate "safety glasses" to protect the remaining eye of monocular patients. Others do not routinely do so when the remaining eye is emmetropic since they feel compliance is poor. To evaluate compliance with safety glasses wear in these children, we reviewed the charts of all children (< 21 years old) treated with enucleation and followed at the University of Iowa by the Pediatric Ophthalmology Service between 1962 and 1991. Safety glasses were defined as polycarbonate lenses in a frame suitable for protective wear with spherical equivalent < or = -0.75 or < or = +2.00 diopters. Fifty-six patients were followed after enucleation. Forty-six of these patients met the inclusion criteria and 33 patients were available for follow up. Eighty-five percent of patients wear safety glasses > or = 50% of their waking hours; 61% wear them > or = 80% of the time; 33% wear them 100% of their waking hours. Twenty-one of the 33 patients participate in sports. One hundred percent of the female participants and 80% to 93% of the male participants wear safety glasses, goggles, or face shields during sports. Fifteen of the 33 respondents reported at least one potentially serious accident in which the safety glasses had protected the good eye. Compliance with safety glasses wear appears to be good in this population and may prevent injury to the remaining eye.

Adolescent↗

PubMedDate not supplied

Compliance with safety glasses wear in monocular children.

1. To evaluate compliance with safety glasses wear in these children, we reviewed the charts of all children (< 21 years old) treated with enucleation and followed at the University of Iowa by the Pediatric Ophthalmology Service between 1962 and 1991. 2. Fifteen of the 33 respondents reported at least one potentially serious accident in which the safety glasses had protected the good eye. 3. Compliance with safety glasses wear appears to be good in this population and may prevent injury to the remaining eye. We recommend prescribing polycarbonate safety glasses for all monocular and functionally monocular children regardless of refractive error or gender.

Adolescent↗