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Biomedical subjects

S Stefani

Publications and source records attributed to S Stefani.

At least 109 records · Page 6Linked to original sources
PubMed1985

Susceptibility of some Staphylococcus species to aminoglycosides.

The Staphylococcal strains, identified by "The Simplified Lyogroups System" were tested for their susceptibility to methicillin and some aminoglycosides. The results, besides showing a higher ratio of susceptibility against aminoglycosides in methicillin-susceptible (MS) strains, show a different trend within each lyogroup. A total of 1616 wild Staphylococcus strains were isolated in microbiological units in Catania, Messina, Rome and Genoa. The results show a high susceptibility to aminoglycosides, both in MS and methicillin-resistant (MR) groups but with different trends among lyogroups.

Amikacin↗
PubMed1980

An evaluation of lymphography with nodal biopsy in localized carcinoma of the prostate. URO-Oncology Research Group.

One hundred forty-nine lymphograms from patients with localized carcinomas of the prostate were interpreted by a referee radiologist without the benefit of clinical stage, grade of tumor, or the result of staging pelvic node dissection. The lymphogram was able to detect 30 of 35 (86%) positive nodal metastases and confirm 90 of 114 (79%) negative nodal biopsies. The internal iliac nodal biopsies were positive as a single finding in only 7 of 54 (13%) patients. The total external iliac nodal involvement was 41 of 54 (76%). High-grade prostate cancer patients have twice the incidence of positive nodal biopsies and lymphograms. Lymphography detected 43% of positive nodal patients with normal acid phosphatase and 69% of positive nodal patients with elevated acid phosphatase determinations. The lymphogram and tumor grade (prostate) are considered significant potential predictors of lymph node biopsy result. Finally, the incidence of nodal metastasis in localized prostatic cancer from surgical and histologically confirmed lymphogram series is reviewed with the concept of sensitivity and specificity of the lymphogram.

Biopsy↗
PubMed1980

Distant metastases of carcinoma of tonsillar region: a study of 475 patients.

The records of 471 patients with carcinoma of the tonsillar region seen at the Veterans Administration Medical Center, Hines, Illinois, have been reviewed to determine the incidence and location of distant metastases. All patients were male and with histologically proved diagnosis. Seventy-two patients (15%) of 471 and 33(29%) of 115 patients who underwent autopsy were found to have distant metastases. Squamous cell carcinoma was by far the most common cell type (88%); however lymphoepithelioma showed the highest incidence of distant metastases. Lung, liver, and bone were the most common anatomical sites of involvement, and thorough work-up of these organs is highly recommended before initiating definitive treatment and during follow-up care.

Bone Neoplasms↗
PubMed1977

Ultrastructural events in the cytoplasmic death of lethally-irradiated human lymphocytes.

Normal human peripheral-blood lymphocytes were irradiated with a dose of X-rays and processed for electron microscopic examination at different times after irradiation. A localized protrusion of the plasma membrane of the irradiated lymphocytes was observed in samples fixed as early as 15 min after irradiation, suggesting that the injury to the plasma membrane could have occurred during or immediately after irradiation. This was followed by fenestration of the plasma membrane, rarefaction of the cytoplasm and accumulation of cytoplasmic organelles in the centrosphere region. Localized distention of the outer nuclear envelope occurred after 2 hours and invagination of the inner nuclear membrane after 4 hours of irradiation. By 24 hours, the cytoplasmic and nuclear ultrastructural integrity was lost. The study suggested that, for high doses of X-radiation, the plasma membrane of the human peripheral-blood lymphocytes was the most sensitive target.

Cell Membrane↗
PubMed1976

[Importance of atrial rhythm disease in geriatrics].

13 patients aged between 69 and 85 suffering from atrial rhythm disease were examined. Special attention was paid to the relationships between this syndrome and acute cerebral vascular pathology. The series shows the high frequency in arrhythmic atrial disease of episodes of incipient loss of consciousness or authentic syncopal phenomena. Fully 11 patients were subjected.....ed to definitive endocardial stimulation owing to the intensity of the symptomatology. On the basis of these observations, stress is laid on the importance of atrial rhythm disease as a cause of acute cerebral ischaemia in the elderly subject

Age Factors↗
PubMed1976

Immune evaluation of lung cancer patients undergoing radiation therapy.

Immunocompetence, as determined by skin reactivity to five microbial antigens, dinitrochlorobenzene (DNCB), and percent and absolute number of total T and B rosette-forming cells (RFC), was assessed before and up to 180 days after radiotherapy in 29 patients with carcinoma of the lung. Pretherapy evaluation showed a depressed response to microbial antigens (only 38% positive), to DNCB (50% positive), and lower level of T-RFC. Postradiotherapy determinations were further depressed. Patients with positive pretherapy responses to microbial antigens and DNCB and higher than median absolute numbers at total T-RFC had significantly longer survival. These data suggest that pretherapy immune evaluation is a good prognostic indicator. No prognostic significance was found in B-RFC evaluation. No correlation was seen between the stage of disease (locally advanced or with distant metastases) and the pretherapy immune response.

Adenocarcinoma↗
PubMed1976

Unification and technical aspects of total t, active t and b lymphocyte rosette assays.

Conditions for rosette formation of human adult T and B lymphocytes with unsensitized and complement coated SRBC respectively, have been examined in this collaborative study. Modified procedures for identifying, by rosette formation, the total T, active T and B-RFC lymphocytes are described. The procedures reported here yielded results for the percent of peripheral blood lymphocytes forming total T, active T and B-RFC for control individuals that are comparable with those previously reported. The advantage of the procedures described here are that they are 1) easy to perform; 2) highly reproducible; 3) allow for the preparation of a single lymphocyte concentration and common reaction mixture volume to be used in all three rosette assays and 4) allow for the removal of non-lymphocytic cells which would interfere with the determination of B-RFC. The use of these procedures would allow other laboratories to obtain similar values so results for controls and various types of patients could be compared.

Adult↗
PubMed1975

A modified assay for the detection of human adult active rosette forming lymphocytes.

A modified procedure is described for the detection of a sub-population of peripheral blood T-cells in normal human adults. This sub-population of T-cells has been called the active rosette forming cell by others. The advantages of this modified procedure compared with that previously described are: 1) the elimination of 60 mon incubation period for lymphocytes prior to the assay and 2) the elimination of the need for fetal calf serum in the assay. The mean percentage of RFC in the peripheral blood of 80 healthy adult controls using the modified procedure is 25.2+/-5.5% in buffer lacking FCS. The results were highly reproducible and comparable to the results obtained using the procedure previously described. It was concluded that a 60 min pre-incubation of lymphocytes and the use of FCS are not necessary when the appropriate SRBS: lymphocyte ratio is used in the determination of human adult active RFC. The use of this procedure in evaluating the immune competence of cancer patients is discussed.

Adult↗