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S Sturrock

Publications and source records attributed to S Sturrock.

8 recordsLinked to original sources
PubMed2001

Specific targeting, biodistribution, and lack of immunogenicity of chimeric anti-GD3 monoclonal antibody KM871 in patients with metastatic melanoma: results of a phase I trial.

PURPOSE: KM871 is a chimeric monoclonal antibody against the ganglioside antigen GD3, which is highly expressed on melanoma cells. We conducted an open-label, dose escalation phase I trial of KM871 in patients with metastatic melanoma. PATIENTS AND METHODS: Seventeen patients were entered onto one of five dose levels (1, 5, 10, 20, and 40 mg/m2). Patients received three infusions of KM871 at 2-week intervals, with the first infusion of KM871 trace-labeled with indium-111 (111In) to enable assessment of biodistribution in vivo. Biopsies of metastatic melanoma sites were performed on days 7 to 10. RESULTS: Fifteen of 17 patients completed a cycle of three infusions of KM871. No dose-limiting toxicity was observed during the trial; the maximum-tolerated dose was therefore not reached. Three patients (at the 1-, 5-, and 40-mg/m2 dose levels) developed pain and/or erythema at tumor sites consistent with an inflammatory response. No normal tissue uptake of 111In-KM871 was observed, and tumor uptake of 111In-KM871 was observed in all lesions greater than 1.5 cm (tumor biopsy 111KM871 uptake results: range, 0.001% to 0.026% injected dose/g). The ratio of maximum tumor to normal tissue was 15:1. Pharmacokinetic analysis revealed a 111In-KM871 terminal half-life of 7.68 +/- 2.94 days. One patient had a clinical partial response that lasted 11 months. There was no serologic evidence of human antichimeric antibody in any patient, including one patient who received 16 infusions over a 12-month period. CONCLUSION: This study is the first to demonstrate the biodistribution and specific targeting of an anti-GD3 antibody to metastatic melanoma in patients. The long half-life and lack of immunogenicity of KM871 makes this antibody an attractive potential therapy for patients with metastatic melanoma.

Adult↗
PubMed1996

Trabeculectomy in the second eye.

Trabeculectomy has a reported success rate of between 67-94%. Many studies have been performed on operative variables which may influence surgical outcome. Youth, pigmented race and anterior segment inflammation have been shown to be associated with trabeculectomy failure. Little is known of the surgical outcome of trabeculectomy in one eye in relation to its partner, when surgery is standardized and the above known adverse conditions to trabeculectomy success are absent. We therefore carried out a prospective study on 62 patients who underwent bilateral trabeculectomies. There was a significant failure rate amongst pairs of eyes (postoperative intraocular pressure > or = 21 mmHg), with the majority of failures occurring within 6 months of surgery. Failure appeared to be associated with a longer period from diagnosis of glaucoma to surgery, and the use of a wider variety and number of topical medications. All other trabeculectomy complications occurred more commonly in any single eye, than in any pairs of eyes.

Aged↗
PubMed1976

Variations of H, A and B antigen strengths among Melanesians.

Melanesians of groups O, A1 and B all show greater variation of H antigen strength than do Caucasians. This is least marked in O bloods. Although the average H content of Melanesian and Caucasian samples did not differ significantly, 10% of O and over 20% of A1 and B bloods from coastal Austronesian speakers had higher H than was found among Caucasians, There is evidence that some non-Austronesian populations in New Guinea have twice this incidence of A1 high H. Melanesian and Bantu H, A1 and B genes appear to function similarly, in that their B genes act more effectively than Caucasian, their A1 genes less so, while H produces greater variation. It is suggested that this gene interaction, rather than the significantly greater availability of H or substrate in the Bantu, causes the observed variations from the Caucasian pattern found in the other races. Subjects with selective antigenic depression were excluded from the comparative studies, but the relevance of this phenomenon to apparent H content in Melanesians is discussed.

ABO Blood-Group System↗
PubMedDate not supplied

Molecular analysis in the diagnosis of pediatric lymphomas.

Thirty-five pediatric lymphomas were categorized as either Burkitt's lymphoma (BL), lymphoblastic lymphoma (LL), or large cell anaplastic lymphoma (LCAL) by histological and immunophenotypic methods. They were further characterized by molecular analysis of their antigen receptor genes. Southern blot (SB) and polymerase chain reaction (PCR) techniques were compared in the detection of immunogloblin heavy chain gene (IgH) rearrangement. T cell receptor beta (TCR beta) rearrangements were analyzed by SB and TCR gamma gene rearrangements by PCR. The PCR method of IgH and TCR gamma gene analysis was preferred to the SB methods, because there were fewer equivocal results in IgH gene analysis, TCR gamma rearrangement was more frequently detected than TCR beta in both lymphoblastic and large cell anaplastic lymphomas, and the PCR technique was more rapid, required less DNA, and could be used with archival material. In addition, analysis of IgH gene rearrangement by PCR was more specific for assessing B cell lineage. Although most of the molecular data were easily interpreted, occasional ambiguous results were seen due to genetic events other than antigen receptor gene rearrangement affecting the genetic analysis. Thus, it is imperative to interpret these genetic data in the context of adequate morphological and immunophenotypic analysis.

Blotting, Southern↗