Biomedical subjects

S Sulkowski

Publications and source records attributed to S Sulkowski.

At least 73 records · Page 4Linked to original sources

PubMed1997

Ultrastructural study of endogenous lipid pneumonia and pulmonary alveolar proteinosis coexistent with lung cancer.

The aim of the study was the ultrastructural analysis of endogenous lipid pneumonia (ELP)-type changes localized in the vicinity of primary neoplasms of the lungs. The analysis was carried out on the material obtained from 30 patients with non-small cell lung carcinoma (NSCLC). Routine histopathological examination was performed in light microscope, focusing mainly on the presence or lack of ELP-type changes. Ultrastructural examinations in transmission electron microscope used 12 cases classified among early (4 cases) or advanced (8 cases) ELP forms. Results of the study reveal a likely coexistence of ELP- and PAP (pulmonary alveolar proteinosis)-type changes in the vicinity of NSCLC and suggest a correlation between the processes leading to the development of ELP or/and PAP. Ethiopathogenesis of the changes observed and the role of granulocytes, macrophages and type II alveolar epithelial cells are discussed.

Carcinoma, Non-Small-Cell Lung↗

PubMed1997

Role of endothelins in lung pathology.

The endothelins are a group of peptides present in a number of tissues and organs. They are powerfully vasoactive, causing both contraction and relaxation of blood vessels. They are also active in the lung causing long lasting bronchoconstriction. Endothelins binding sites are widely distributed and are localized to airway and pulmonary vascular smooth muscle, fibroblasts, submucosal glands, and airway nerves, indicating that endothelins may have widespread effects. The current study presents a potential contribution of endothelins to pathogenesis of a number of lung diseases.

Animals↗

PubMed1997

Protective effect of human recombinant tumour necrosis factor-alpha against lung metastases of the Morris hepatoma in rats.

The effect of intratumour (i.t.) administration of human recombinant tumour necrosis factor-alpha (hrecTNF-alpha) on Morris 5123 hepatoma spontaneous lung metastases was studied. Tumour was implanted in the skeletal muscles of the right hind limb of Buffalo rats. Two weeks after the implantation treatment with cytokine started. The cytokine was injected i.t. in a single dose of 10 micrograms in 4- and 8-day cycles. In control animals PBS was administered, respectively. Then all rats were necropsied and the extent of lung metastases was determined. Data were expressed as volume of lung metastases. In this study we observed significant antimetastatic effects of hrecTNF-alpha (p < 0.05) determined by reduced volume of lung metastases in treated animals.

Animals↗

PubMed1997

Alveolar epithelial cells after intratumour administration of HREC TNF-alpha mutein VI.

The aim of this study was to explore the effect of intratumour mutein VI-Hrec TNF-alpha administration upon the ultrastructural changes within the pulmonary tissue, with special attention paid to type II alveolar epithelial cells. The experiment was carried out on Buffalo rats with implantable Morris hepatoma series 5123 in the skeletal muscles of the limb. Mutein VI-Hrec TNF-alpha was administered in a dose of 10 micrograms once a day in a cycle of eight days. Control animals were given saline (PBS). Ultrastructural changes within the pulmonary tissue were evaluated in the electron transmission microscope (TEM), with special attention paid to alveolar epithelial cells. In the animals receiving hrec TNF-alpha mutein VI, damage to the alveolar epithelial cells was found. In the later period (14 days after the mutein treatment), repairing processes were observed, accompanied by intensified fibrotic processes in the interalveolar septal interstitium, with the subsequent pulmonary tissue rebuilding. The study confirmed the possibility of a peripheral action of hrec TNF-alpha mutein VI after its administration to the experimental Morris hepatoma and found the alveolar epithelial cells to be a key element of the pulmonary tissue subjected to the cytokine effect.

Animals↗

PubMed1997

Comparative studies on the effect of TNF-alpha and endotoxin on the ultrastructural picture of pulmonary capillaries in pregnant rabbits.

The aim of the present study was the comparative analysis of morphological changes found in the pulmonary microcirculation of pregnant rabbits in the course of experimental septic shock induced by endotoxin or human recombinant TNF-alpha administration. The experiments used 30 female rabbits, white Dutch, c.3 kg mean body weight. The endotoxin Escherichia coli, serotype S.0127: 138 (Sigma) was applied in a single dose of 100 micrograms/kg b.w. intraperitoneally. The human recombinant TNF-alpha (biological activity 2-4 x 10(7) U/mg of protein) was injected intraperitoneally, also once, in a dose of 100 micrograms/kg b.w. Morphological examinations were based on the ultrastructural analysis in the transmission electron microscope. The ultrastructural analysis revealed no morphological differences between pregnant and non-pregnant rabbits which received TNF-alpha. However, significant differences were observed between animals given TNF-alpha and those given the endotoxin. These differences referred to endothelium damage degree and to the cellular composition of inflammatory infiltrations. More severe damage to endothelial cells (necrosis inclusive) was observed in the endotoxin-treated rabbits. Blood vascular lumen in these animals showed cellular aggregation, of neutrophils and platelets in particular as well as microthrombi. An increased tendency towards the development of these changes was noted in pregnant rabbits. The lumen of pulmonary capillaries in TNF-alpha treated animals showed domination of monocytic cells. Features of endothelial cell stimulation were observed, although without a tendency to form microthrombi.

Animals↗

PubMed1997

Quantitative and morphological changes in BAL-isolated cells after the administration of TNF-alpha into Morris hepatoma.

The aim of this study was to explore the effect of intratumor TNF-alpha administration upon the composition and adherence degree of cells isolated from the lungs through multiple bronchoalveolar lavages (BAL). Ultrastructural evaluation of BAL-isolated cells was performed in the scanning electron microscope (SEM). The experiment used Buffalo rats. A suspension of 3 x 10(6) cells of Morris hepatoma (5123 series) was injected to the right hind leg of the animals. After fourteen days, TNF-alpha was administered into the tumor in a dose of 1.5 x 10(4) U in 0.5 ml PBS solution. The animals of group I were given 4 doses of TNF-alpha and group II-8 doses of TNF-alpha every 24 hours. Control groups consisted of rats with injected Morris hepatoma which were given PBS solution instead of TNF-alpha (group III A, B) and animals without the hepatoma, given intramuscullary 4 or 8 TNF-alpha, respectively (groups IV A, B). No statistically significant differences were noticed between groups I and II with regard to the number of macrophages and neutrophils isolated from the rat lungs compared with control group IV. However, such differences were observed compared with group III. In group II and IV B, an increase in the adherence of isolated cells was found compared with group III, as well as arise in the number of macrophages with the largest diameters. We found a correlation between the increase in cell adherence and ultrastructural changes (in SEM) suggesting an increased activity of BAL-isolated cells.

Animals↗

PubMed1997

Contribution of capillary endothelium to the processes of pulmonary tissue rebuilding in the course of acute enzymatic lung injury.

Ultrastructural analysis was made of the pulmonary capillary endothelial cells, with special regard paid to their possible contribution to the processes of pulmonary tissue rebuilding initiated by intratracheal infusion of a proteolytic enzyme-papain. Experimental animals (male Wistar rats) were additionally given BCG vaccine in doses activating the alveolar macrophage system. The study was performed after 1 day, and 1, 4 and 12 weeks following papain administration. In the animals given BCG vaccine, ultrastructural exponents of endothelial cell activation were found. Cumulation of inflammatory cells, mainly monocytes, was observed in vascular lumen. Application of papain solution to animals caused a number of damaging and exudative changes, being most pronounced within vascular endothelium in animals simultaneously given BCG vaccine. In later periods, rebuilding and productive processes, accompanied by collagen cumulation in the interstitium of interalveolar septa, dominated. In the animals receiving BCG vaccine and papain, ultrastructural pictures indicated active contribution of endothelial cells to the processes of pulmonary tissue rebuilding initiated by intratracheal infusion of the proteolytic enzyme.

Animals↗

PubMed1997

Ultrastructural analysis of the pneumocyte-interstitium boundary line in the course of enzymatic lung injury.

The studies were performed basing on the experimental model of acute pulmonary tissue injury. Papain in a dose of 2 mg/ml PBS/100 g b.w. was administered once, intratracheally, on the 21st day of the experiment. Besides, female Wistar rats were injected twice with BCG vaccine in a dose of 4 x 10(8) microorganisms. BCG vaccine was administered intraperitoneally on the 1st and 14th day of the experiment to activate the system of mononuclear phagocytes. Control rats were intratracheally or/and intraperitoneally given PBS solution. All the animals were killed on the 28th, 35th and 42nd day of the experiment. A single intratracheal papain injection induced emphysematous changes in the animal lungs. The changes were accompanied by basement membrane rebuilding and focal collagen and elastin cumulation. An increase in the number of type II alveolar epithelial cells was observed. Anchorage of collagen fibres and microfibrillary structures in the cytoplasm of type II pneumocytes was observed in the BCG- and papain-treated animals. There, the cytoplasmic membrane of type II cells was completely indistinct and the cytoplasm formed processes to penetrate into the connective tissue fibres. The results obtained indicate possible contribution of type II pneumocytes to fibroplasia processes during lung parenchyma rebuilding and suggest the necessity to include fibroplasia elements in the existing definition of emphysema.

Animals↗

PubMed1997

Evaluation of the effect of macrophage system activation on the intensity degree of early destructive changes in acute enzymatic lung injury.

The effect of macrophage system activation on the intensity degree of early destructive changes induced with intratracheal infusion of a proteolytic enzyme-papain was evaluated in the paper. The study was carried out on male Wistar rats. BCG vaccine was used as a macrophage system activating substance. Damage degree of the pulmonary tissue was evaluated after 2 hours following papain administration by determining W/D index (wet lungs/dry lungs). Spectrophometric analysis of bronchoalveolar lavages (BAL) was made in order to evaluate the advancement of haemorrhagic alterations in the lungs. Damage to the pulmonary tissue was greater in the animals given BCG vaccine and papain, compared with controls and papain treated animals. Differences in the intensity degree of destructive changes assessed using both the W/D index and haemoglobin content in BAL were statistically significant. The results obtained indicate a particular role of activation of the macrophage system in the development of early destructive changes due to intratracheal administration of proteolytic enzymes and point to the significance of these changes in the morphogenesis of experimental lung emphysema.

Animals↗

PubMed1997

Regression of Morris hepatoma in response to intralesional treatment with tumor necrosis factor muteins.

We examined the antitumor effects of human recombinant tumor necrosis factor alpha (rhTNF-alpha) and its muteins with the N-terminal amino acid sequence altered by point mutations against transplantable Morris hepatoma 5123 in rats. In vivo studies showed antiproliferative activity of the drugs in the dose range tested. For in vivo studies rhTNF-alpha and muteins were administered intratumorly (i.t.). The preparations were given at a dose of 10 microg/rat, once daily for eight days. Although the therapy was significantly effective in inhibiting tumor growth, complete growth inhibition could not be achieved. Nevertheless, there was a significant increase in survival time of tumor-bearing rats.

Amino Acid Sequence↗

PubMed1997

Surfactant system in lung cancer. Endogenous lipid pneumonia.

The aim of the study was the evaluation of endogenous lipid pneumonia-type changes developing in the vicinity of primary tumors of the lungs. The postoperative material collected from 56 patients operated for non-small cell lung cancer was examined. The coexistence of endogenous lipid pneumonia-type changes with squamous cell carcinoma and large cell carcinoma of the lung was most common. Patients with these histopathological types of carcinoma had lower percentage of metastases to the lymph nodes. Endogenous lipid pneumonia was found to accompany most frequently the tumors 3 < or = 6 cm in diameter and it was slightly more common in the vicinity of peripheral tumors.

Carcinoma, Non-Small-Cell Lung↗

PubMed1997

[Scanning electron microscopy (SEM) of subretinal fluid in idiopathic retinal detachment].

PURPOSE: To evaluate the cells of subretinal fluid (SRF) by the use of the scanning electron microscope and to determine relationships between morphologic features of SRF and duration of retinal detachment. MATERIALS AND METHODS: Subretinal fluid was taken from 7 patients with retinal detachment. The cytologic preparations were analyzed in electron scanning microscope (IEOL). RESULTS: The highest count of cells (predominantly macrophages and lymphocytes) were seen in SRF obtained in the third week after retinal detachment. Retinal detachment, which lasted more than 3 weeks, caused lower SRF cells count and changes in cells composition. After 6 months normal cells were not present in SRF.

Adult↗

PubMed1997

The effect of pentoxifylline on ultrastructural picture of type II alveolar epithelial cells and generation of reactive oxygen species during cyclophosphamide-induced lung injury.

The aim of the study was to evaluate the effect of pentoxifylline (PTXF) on the production of reactive oxygen species in the rat lungs after intraperitoneal (i.p.) cyclophosphamide (CP) administration (150 mg/kg b.w.) and to draw a correlation between the morphological changes and biochemical findings. Morphological examinations were based on ultrastructural analysis in the transmission electron microscope. It was found that single i.p. CP administration caused destructive changes, particularly within mitochondria and lamellar bodies of type II alveolar epithelial cells (EP II), and within blood vessels of interalveolar septa. The changes were accompanied by an increase in the MDA (malondialdehyde) homogenates. The animals receiving PTXF + CP showed normalization of MDA level and minor damage to EP II confined to alterations in lamellar bodies of EP II. The vascular changes observed after i.p. CP administration were manifested in endothelial swelling and accumulation of neutrophilic granulocytes, monocytes and in the later period blood platelets within the capillaries of interalveolar septa. The cells were tightly attached to endothelium. The changes in the vascular system were accompanied by a significant decrease in the activity of antioxidant enzymes (Cu,Zn-SOD and GSSG-R) in the blood serum collected from the left ventricle of the heart. In the animals given PTXF + CP, a higher decrease was revealed in the activity of these enzymes. Ultrastructural examinations found morphological signs of a facilitated flow, mainly of cellular elements, through the vessels of the interalveolar septa of the lungs.

Animals↗

PubMed1997

Tumor necrosis factor-alpha induces emphysema-like pulmonary tissue rebuilding. Changes in type II alveolar epithelial cells.

The effect of repeated doses of TNF-alpha on the histological picture of the pulmonary tissue was analyzed in the present study. Special attention was paid to the lung rebuilding processes. TNF-alpha was applied intraperitoneally for two weeks in a dose of 10 micrograms/0.5 ml PBS/24h. Morphological analysis of the pulmonary tissue was performed after 1 and 28 days following the last TNF-alpha dose. The study revealed focal pulmonary tissue rebuilding with emphysema-like changes twenty eight days following termination of TNF-alpha administration. The rebuilding processes included interalveolar septal atrophy, collagen accumulation and damage-repair changes in type II alveolar epithelial cells. It has been demonstrated that apart from the protease-antiprotease hypothesis of the lung emphysema, the inflammatory-repair hypothesis should be considered. Both hypotheses are complementary to each other and interpret the emphysema-like changes as complications of various pathological conditions of the pulmonary tissue.

Animals↗

PubMed1997

Ultrastructural studies of the effect of pentoxyfylline on the hepatic cell of the rat with simultaneous administration of cyclophosphamide.

Ultrastructural examinations carried out on liver specimens collected from Wistar rats revealed that single administration of cyclophosphamide in a dose of 150 mg/kg body weight produced changes in the cytoplasmic structures, particularly in the smooth endoplasmic reticulum and mitochondria. The changes were reversible and subsided 14 days following cyclophosphamide administration. Administration of pentoxyfylline to rats in a dose of 30 mg/kg b.w./day for 10 days induced structural alterations in the intracytoplasmic structures of hepatocytes, similar to those caused by cyclophosphamide, although more intensified in the vicinity of the vascular pole. The pentoxyfylline-induced changes in hepatocytes retreated already 4 days after its withdrawal and can be considered adaptative. Simultaneous administration of both drugs to rats resulted in enhanced metabolic disturbances in hepatocytes and dissociation of adaptative mechanisms manifesting themselves in irreversible morphological changes in the intracytoplasmic structures and in the occurrence of collagen in the intercellular spaces.

Animals↗

PubMed1997

Effects of pentoxifylline on some peripheral blood parameters and haemostasis in acute pulmonary tissue injury after cyclophosphamide.

Cyclophosphamide (CP) is one of the widely used cytostatic drugs, with a strong toxic influence on pulmonary tissue. Experimental works have shown that a single high dose of CP causes injury to all elements of the interalveolar septum, especially to the alveolar endothelial and epithelial cells. The aim of this work was to evaluate the influence of pentoxifylline (PTXF) (30 mg/kg b.w.) on the ultrastructure of lungs capillaries and blood cell count as well as plasma fibrinogen levels in Wistar rats after intraperitoneal injection of 150 mg/b.w. CP. We established that in the doses applied PTXF had no statistically significant influence on the number of leukocytes and erythrocytes determined in the left ventricular blood of rats receiving CP, while the number of white cells from animals given PTXF only was higher than in controls. There was a smaller decrease in the number of platelets (p < 0.05) and smaller reduction in fibrinogen level (p < 0.01) in the serum of PTXF-CP animals than in the CP group. The results obtained suggest a protective effect of PTXF on CP induced changes, which have been evidenced in some of the parameters examined. Ultrastructural examinations found the lungs to be the organ of extramedullary thrombocytopoiesis in CP-treated animals and revealed that platelet accumulation in the system of lung capillaries was a potential cause of the decrease observed in the number of blood platelets following CP administration.

Animals↗

PubMed1997

Microenvironment of Morris hepatoma 5123 metastases to the lungs--ultrastructural analysis of intratumour injections of rhTNF-alpha.

We analysed the effect of rhTNF-alpha on the development of Morris hepatoma 5123 spontaneous metastases to the lungs of Buffalo rats. After intratumour administration of the cytokine the lungs were found to contain scarce metastatic foci. In precapillaries and capillaries neoplastic cells were single or in small clusters, without contact with endothelial cells. Endothelial cells showed high pinocytic activity and frequently tightly closed vascular lumen. The stroma of interalveolar septa and the capillary bed contained multiple eosinophils. Moreover, eosinophils, histiocytes and monocytes mixed with abundant collagen fibres were found on the margin of single small metastatic foci and among necrotic neoplastic cells.

Animals↗

PubMed1996

Changes of myocardial capillary density in progression of experimental lung emphysema.

A morphometrical analysis of changes in the heart of rats with experimental lung emphysema due to single intratracheal administration of papain solution has been done. Special attention has been paid to quantitative changes of capillaries and to the cardiomyocyte diameter in respective regions of the myocardium after one, three and six months following the administration of papain. A histological and morphometrical analysis of changes in the lungs has also been carried out and partial pressures of oxygen and carbon dioxide in the arterial blood of the examined animals have been marked. It has been found that a single administration of papain caused emphysematous changes in the lungs, growing more intense until the third month of the experiment. The progression of these changes corresponded to a capillary density increase in the right ventricle, interventricular septum and in the subendocardial layer of the left ventricle. The increase in the number of capillaries was interpreted as the first adaptive stage of cor pulmonale development and explained by the occurrence of angiogenesis. The changes co-existed with a decrease in partial pressure of oxygen in the arterial blood. After six months of the experiment, a reduction in capillary density in the heart regions mentioned above and a simultaneous increase in the cardiomycyte transversal diameter were observed, being the exponents of evident myocardial hypertrophy. Development of ultrastructural changes in the hearts of rats has been analysed too. Simultaneous development of changes in the right ventricle muscle and in the subendocardial layer of the heart left ventricle myocardium was observed. The role of hypoxia as a one of essential factors responsible for the observed morphologic changes has been discussed.

Animals↗