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S Szymaniec

Publications and source records attributed to S Szymaniec.

At least 19 recordsLinked to original sources
PubMed2005

[Retinal vein branch occlusion and palsy of the N. abducens in protein S deficiency].

BACKGROUND: Protein S deficiency, which exists in 0.7% of the population, is a risk factor for retinal vein branch occlusions and is inherited in an autosomal dominant manner. METHODS: A genealogical study was carried out on three generations of one family who exhibited different venous occlusions and subsequent complications. RESULTS: Four members of the family, spanning three generations, suffered from complications of venous thrombosis. In the first generation a great uncle died of complications from a deep leg venous thrombosis. In the second generation, the mother underwent a venous branch thrombosis at the age of 41 with a protein S activity of 18%. Subsequently, a palsy of the N. abducens developed with multiple cerebral lesions (presumably post-thrombotic) in the MRI. Fluorescein angiography showed a typical picture of a venous branch occlusion which had been treated by laser. In the third generation, the 16-year-old daughter developed iliac venous thrombosis and a pulmonary embolism with a protein S activity of 0%. The fluorescein angiography showed distinctively engorged veins. A 28-year-old daughter, with a protein S activity of 16%, remained asymptomatic, although fluorescein angiography demonstrated engorged veins. Protein C activity and APC resistance of all family members were normal. The chromosomal analysis of the family members revealed no morphological aberrations. CONCLUSION: Protein S deficiency increases the risk of congenital thrombosis in young and middle-aged heterozygous individuals.

Abducens Nerve Diseases↗
PubMed2001

[VEGF, TNF-alpha and IL-6 in the peritoneal fluid of women with unexplained infertility].

Unexplained infertility is an important problem in diagnosis and therapy in everyday gynecologist's practice. Looking for possible reasons of the unexplained infertility we studied the concentrations of selected cytokines (VEGF, TNF-alpha and IL-6) in the peritoneal fluid of women suffering from the unexplained infertility. We compared the results in the studied group with the control group and with the patients with endometriosis. Immunological disorders of the peritoneal fluid in endometriosis are thought to take part in its pathomechanism. Our results suggest that the levels of one of the main factors of endothelium proliferation (VEGF) in the peritoneal fluid from women with unexplained infertility and women with endometriosis are comparable. Concentration IL-6 and TNF-alpha in the peritoneal fluid in case of unexplained infertility and control group was lower than in the endometriosis patients.

Adult↗
PubMed2001

Novel nociceptin analogues.

A series of new nociceptin analogues containing cysteine was tested for their nociceptive effects in tail-flick test on mice after icv injection. The cysteines were introduced in order to get irreversibly binding analogues based on the assumption that the cysteines in the ligand can interact with the cysteines from the receptor to form an S-S bridge. In vivo tests revealed that Cys1-nociceptin (1-13)-NH2 (Cys1-NC) is an antagonist, whereas Cys7-NC is an agonist. Gly1[Phe(p-NO2)]4-NC was less active indicating that the antagonist properties of Cys1-NC are associated with the presence of the sulfhydryl group of cysteine. The analogues D-Cys2 and Cys3 were also almost inactive.

Amino Acid Sequence↗
PubMed1998

[Cytotoxic activity of NK cells in the presence of the peritoneal fluid fro women with unexplained infertility and with endometriosis related infertility].

We examined peritoneal fluid obtained during laparoscopy in the aspect of its influence on cytotoxic activity of NK cells. We showed significant suppression of this activity in women with unexplained infertility. The suppression of NK activity was also present in women fertile and infertile both, with severe endometriosis. These results may indicate the immunological disorders in unexplained infertility and in endometriosis-related infertility.

Ascitic Fluid↗
PubMed1998

Oral treatment of rats with bovine lactoferrin inhibits carrageenan-induced inflammation; correlation with decreased cytokine production.

The aim of this study was to investigate an effect of oral treatment of rats with bovine lactoferrin (BLF) on carrageenan-induced inflammation. Rats were given 5 oral doses of BLF (10 mg each) on alternate days and 24 h after the last dose a carrageenan inflammation was induced in the hind foot. Control rats were given 0.9% natrium chloride (NaCl) or bovine serum albumin (BSA). The magnitude of the reaction was measured after 2 h (optimal response) and expressed as an increase of the foot pad thickness in milimeters. The evaluation of BLF effects on carrageenan reaction was supplemented by determination of the ability of spleen cell cultures to produce interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) upon lipopolysaccharide (LPS) induction using bioassays. The results revealed an inhibition of the carrageenan-induced inflammation in BLF-treated rats by 50 and 40% as compared to NaCl and BSA control groups, respectively. The inhibition was also associated with a substantial decrease in the ability of splenocytes to produce IL-6 in BLF-treated rats (94 and 83% as compared to NaCl- and BSA-treated groups). The LPS-induced TNF-alpha production was also decreased, although to a lesser degree (48 and 35%, respectively). The decreased ability of spleen cells to produce inflammatory cytokines in BLF-treated rats indicates that hyporeactivity of the immune system cells may be the basis for the inhibition of carrageenan-induced inflammation.

Administration, Oral↗
PubMed1995

Effect of benzisoselenazolones and organic diselenides on graft versus host reaction and immunoglobulins levels in chickens.

Two week old chickens were treated once daily for 5 days with AE8--1-pyridyl-1,2-benzisoselenazol-3(2H)-one, AE22--bis-2-(N-phenyle-carboxamido) 1 pyridyl diselenide and AE31--bis(phenylo-diselenide with R3 = CONHC18H37). Their whole blood alone or blood mixed with thymus cells were used to generate graft versus host (GvH) reaction in 15 day old chicken embryos. The treatment of the chickens with the compounds stimulated the GvH reaction modifying activity of the donor cells as measured by increase of the spleen weight of the recipient chicken embryos. On the other hand, treatment with these compounds inhibited the IgG or IgM production in chickens immunized with human albumin.

Animals↗
PubMed1995

Immunotropic activities of benzisoselenazolones and organic diselenides in mice.

We have investigated the immunotropic effects of 23 seleno-organic compounds (8 benzisoselenazolones, 3 benzisoselenazolone oxides and 12 organic diselenides). All of the compounds increased the rosette formation of sheep red blood cells (SRBC) with spleen cells obtained from thymectomized C53BL/6 mice and incubated in vitro in the presence of imuran. Furthermore, 16 of the compounds were also assayed in vitro in the hydrocortisone test performed with C57BL/6 mouse thymocytes. It was found that all of them significantly protected the cells against hydrocortisone induced cytotoxicity. Also in the Jerne's assay, performed in 129Ao/Boy mice pretreated in vivo with 3 selected compounds 5 days before immunization with SRBC, the stimulation of plaque forming cells (PFC) was observed. Only one compound (AE22, an analog of piroxicam) was found to be inhibitory in this assay. In contrast, in the graft versus host (GvH) assay performed in hybrid mice the donor lymphoid cells obtained from C57BL/6 mice pretreated with 9 selected seleno-organic compounds, suppressed the GvH reaction in the recipient hybrid mice. Thus, in all of the immunotropic assays except the GvH reaction in adult mice, the seleno-organic compounds were found to have immunostimulating activities.

Adjuvants, Immunologic↗
PubMed1994

Immunotropic activity of lupin seeds extracts and fractions from Lupinus angustifolius and Lupinus albus.

The results demonstrated immunotropic activity of seeds extracts and fractions from Lupinus angustifolius and Lupinus albus. Plaque forming cells (PFC) number to SRBC (sheep red blood cells) were elevated by an extract from Lupinus angustifolius and lowered by extracts from Lupinus albus. All preparations obtained from Lupinus angustifolius reduced the number of rosette forming cells (E-RFC). These preparations suppressed also the intensity of graft versus host reaction (GVHR) in case when the donors were treated. Lupinus albus extract suppressed GVH reaction when recipients were treated. Lupin extracts stimulated draining popliteal lymph nodes in PLN assay.

Adjuvants, Immunologic↗
PubMed1989

Antibody producing cells in the spleens of mice treated with pathogenic mineral dust.

Experiments were carried out to assess the effect of intraperitoneal injection of the mineral dusts, titanium dioxide, quartz, or asbestos, on splenic lymphocyte antibody forming cells in immunised mice. Titanium dioxide and quartz caused similar, about one third, reductions in plaque forming cells; asbestos caused substantial reduction to about a quarter of the number found in control spleens. The inhibition of antibody forming cells in the spleen found with chrysotile was dose dependent and both chrysotile and crocidolite asbestos were similar in activity. Systemic immunomodulation after local deposition of mineral dust may be important to the development of disease.

Animals↗
PubMed1988

Human immunodeficiency virus (HIV) antibodies in patients with haemophilia and other inheritable blood clotting disorders in south west Poland (Lower Silesia region).

In order to determine the frequency of HIV antibodies among patients with haemophilia and other inheritable blood clotting disorder in the Lower Silesia region of South-West Poland, we examined a representative fraction comprising 106 patients from a cohort of 302 blood clotting disorder patients. The methods used for detecting HIV antibodies were an ELISA, the immunoblotting test ("Western blot") and an indirect immunofluorescence test. 8 patients were reactive in the ELISA, but only 2 (1.9%) patients were truly seropositive by the confirmation tests. Both patients have received imported commercial factor VIII preparation. Totally 17 patients have been treated with imported factor VIII, thus 12% (95% confidence limits: 1.5-36.5%) of these were seropositive. This figure, which is lower than figures obtained from the USA and Western Europe, is probably due to far less intensive treatment of the patients. None (95% confidence limits: 0-4.1%) of the patients solely treated with blood transfusion and locally produced preparation was HIV antibody positive. These results indicate a low prevalence of HIV infection in this region of Poland but are, on the other hand, a strong argument for an introduction of HIV antibody screening of blood donors to maintain these favourable condition.

Acquired Immunodeficiency Syndrome↗
PubMed1987

Human seminal plasma suppresses lymphocyte responses in vitro in serum-free medium.

The in vitro immunosuppressive properties of human seminal plasma have been re-investigated in serum-free medium in view of recent suggestions that the previously observed effects might be dependent on the presence of exogenous serum co-factors present in the culture media. The present studies reveal that low concentrations of seminal plasma can inhibit the ability of peripheral blood leukocytes to lyse K562 target cells in the absence of fetal calf or new-born calf serum. These inhibitory effects could be achieved by pre-incubating the effector cells in seminal plasma at 37 degrees C prior to use in the natural killer cell assay or by incorporating it into the assay system. Additional studies revealed that human seminal plasma could also inhibit the proliferative response of peripheral blood lymphocytes to phytohaemagglutinin in serum-free HB103 medium. These effects were most marked and consistent if the seminal plasma was present throughout the period of culture. Overall, these studies indicate that the previously reported suppressive effects of human seminal plasma in these systems cannot be entirely attributable to cytotoxic factors generated by exogenous serum components.

Culture Media↗
PubMed1987

Studies on the immunosuppressive effect of seminal plasma.

In vitro suppression of immune responses by seminal plasma is well documented, but the mechanism by which it exerts its effects remains to be established. Our studies on T-lymphocyte proliferation and natural killer cell target-cell lysis reveal that seminal plasma mediated suppression is dose-dependent and temperature-dependent, and that cells which have been activated are less susceptible to suppression. In the case of mitogen-induced T-cell responses this results in a decrease in the expression of the Interleukin-2 receptor whose generation is essential to T-cell proliferation. These studies provide further evidence about suppression of the immune response by seminal plasma. This may be a contributory factor in the aetiology of AIDS, other sexually transmitted diseases, infertility and malignancies of the urogenital tract including carcinoma of the cervix.

Cell Division↗
PubMed1986

Quantitative analysis of tissue distribution of Ly10-like murine alloantigen(s) with antisera and monoclonal antibodies.

Tissue distribution of non-Lyt1.1 ("Ly10-like") antigen or antigens encoded by short chromosomal segment differentiating B6-Ly-1a congenic strain from B6 strain of mice was studied by quantitative absorption of (BALB/c X B6)F1 anti B6-Ly-1a antiserum and by direct cytotoxicity of Ly-10-132-12-26 monoclonal antibody on lymphoid cell populations. Identical strain but not tissue distribution pattern does not allow to conclude whether antiserum and monoclonal antibody detect the same or closely linked antigens. Absorption experiments revealed the highest antigen content in the brain tissue, lower in testis and kidney, still lower in lymphoid organs and the lowest in liver and lung. Among lymphoid cells, bone marrow cells had highest absorbing capacity, followed by thymus, spleen and lymph nodes. Monoclonal antibody lysed almost 100% of thymocytes, 30% bone marrow cells and 10-20% of spleen and lymph node cells (both T-cell and B-cell enriched populations contained the same proportion of positive cells). Cortisone resistant thymocytes showed the same sensitivity as cortical thymocytes to Ly-10-132-12-26 antibody which is distinguishable characteristics of medullary thymocytes from peripheral T cells. Mitogen activated lymphocytes exhibited significantly higher expression of Ly10-like antigen than resting peripheral lymphocytes.

Animals↗
PubMed1985

Human seminal plasma is a potent inhibitor of natural killer cell activity in vitro.

The effect of human seminal plasma on the natural killer (NK) activity of human peripheral blood leukocytes in vitro has been investigated. Small concentrations (as low as 0.01% v/v) of seminal plasma were found to inhibit the ability of human peripheral blood leukocytes to lyse K562 target cells. These inhibitory effects were observed with seminal plasma donations from normal and infertile individuals, including those who had been vasectomised. The inhibition noted appeared to be independent of the presence of antisperm antibodies which could conceivably cross-react with NK cells. Furthermore, the factors responsible survived boiling for 15 min and could not be absorbed out with K562 target cells. These profound inhibitory effects might be relevant to malignancy of the urogenital tract and the defence against sexually transmitted disease.

Autoantibodies↗
PubMed1984

Effect of dichloromethylene diphosphonate (Cl2-MDP) on bone resorption induced by PHA-stimulated human mononuclear cells in organ culture.

Human mononuclear cells stimulated by PHA caused resorption of fetal rat bones in tissue culture. Non-adherent cells alone failed to produce substantial bone resorbing activity. The mixed culture of adherent and non-adherent cells (1:40) produced significant bone mineral resorption in vitro. The bone resorption induced by human mononuclear cells stimulated by PHA required the presence of living bone tissue in culture. The bone resorbing effect caused by PHA-stimulated cells was blocked by dichloromethylene diphosphonate at 10(-5) M concentration, but mononuclear cells preincubated in diphosphonate containing media were capable to resorb fetal rat bone in tissue culture.

Animals↗