PubMed Health⌕ Search

Biomedical subjects

S Taguchi

Publications and source records attributed to S Taguchi.

At least 145 records · Page 8Linked to original sources
PubMed1989

Molecular cloning and nucleotide sequence determination of gene encoding Streptomyces subtilisin inhibitor (SSI).

A gene for Streptomyces subtilisin inhibitor (SSI) from Streptomyces albogriseolus S-3253 was cloned into E. coli plasmid pBR322 using two oligodeoxyribonucleotides corresponding to Asp68 to Pro77 and Asn99 to Gly107 of the protein, respectively. The SSI gene was localized on a 1.8-kbp BglII/SalI fragment. The nucleotide sequence of this 1.8-kbp fragment was determined by the dideoxy sequencing method. The amino acid sequence of the mature SSI coding region derived from the nucleotide sequence determination corresponded exactly to that from protein sequencing analysis. The nucleotide sequence analysis showed the presence of a putative signal peptide comprising 31 amino acids preceding the mature SSI region. The major transcriptional start point was identified to be 60 nucleotides upstream from the putative initiation codon for translation by the primer extension method. The -45 to -25 region upstream from transcriptional start point was quite homologous to that of CTC promoter of Bacillus subtilis. The overall G + C content of this 1.8-kbp fragment was 72%. On the other hand, an extremely high G + C content (96%) was found at the third letter of codons in the SSI coding region.

Bacterial Proteins↗
PubMed1989

Relative influence of plaque control, scaling and root planing on occlusal sounds.

Nine periodontally affected subjects and eight healthy subjects were selected in order to examine the effect of initial periodontal treatment on occlusal sounds. Gingival Index, probing depth, tooth mobility and occlusal sound duration via an occlusal sound analyser were recorded at the baseline. The nine periodontal patients were then treated by plaque control, root planing with general scaling and were re-examined at 1 month and 3 months post-operatively. The following results were obtained: (i) The normal duration of an occlusal sound is less than 10 ms. (ii) Occlusal sounds of periodontal patients were of longer duration compared with values in healthy subjects, but decreased and approached the normal value after treatment.

Adult↗
PubMed1989

Plasma secretin, CCK, and pancreatic secretion in response to dietary fat in the rat.

The role of fat in regulation of pancreatic secretion was studied in conscious rats by measuring pancreatic secretion and plasma cholecystokinin (CCK) and secretin responses to intraluminal infusion of fat, protein, or trypsin inhibitor via the duodenum. In rats with pancreatic juice continuously returned to the intestine, intraduodenal infusion of 20% emulsified fat (Liposyn), 10% casein, and 0.4% ovomucoid trypsin inhibitor (OMTI) stimulated equivalent increases of approximately threefold in pancreatic protein output. Proglumide reduced fat-stimulated pancreatic protein secretion by greater than 90% but did not inhibit the response to OMTI. Fat significantly increased plasma CCK from basal levels of 0.5 pM to 2-3 pM, but it was a weaker stimulant of CCK secretion than casein (peak CCK levels greater than 10 pM) or OMTI (peak CCK levels 5-6 pM). Fat significantly stimulated secretin release (21.7 pM) compared with casein (6.8 pM), OMTI (4.4 pM), and NaCl (3.5 pM). The inhibition of fat-stimulated pancreatic secretion by proglumide indicates that the small amounts of CCK released by fat are necessary for a normal pancreatic response, suggesting that this response may be the result of potentiation between secretin and small amounts of CCK.

Animals↗
PubMed1989

[Effects of nicotinate ethyl ester treatment on gingival blood circulation of experimental gingivitis in monkeys].

The effects of nicotinate ethyl ester treatment on the gingival blood circulation of plaque induced inflammation in monkeys were determined. The blood flow, amount of hemoglobin and oxyhemoglobin rate (oxyhemoglobin/total hemoglobin) in gingival blood circulation in addition to bacterial colony forming units (CFU), gingival index (GI), probing depth (PD), loss of attachment (LA) and amount of gingival crevicular fluid (GCF) were used as parameters. Besides 1% nicotinate ethyl ester paste (nicotinate paste), 0.5% chlorhexidine (CHX) paste as an antibacterial agent, a mixture of the two pastes and the base alone as a control were used. 1. The blood flow, amount of hemoglobin, CFU, GCF, PD and GI increased as inflammation developed, and these showed significant correlations. 2. The oxyhemoglobin rate showed no corresponding change with inflammation. 3. Nicotinate paste, CHX paste and the mixed paste seemed to reduce the inflammation of the gingiva as assessed by the blood flow, hemoglobin amount, CFU and GCF amount compared with the control paste.

Animals↗
PubMed1989

Concentration of free thyroxin in serum during nonthyroidal illness--calculation or measurement?

We measured the free thyroxin (FT4) concentration in serum from 79 patients with nonthyroidal illnesses (NTI), using two analog RIAs and two calculation methods, and compared the results with those by ultrafiltration (UF) as a reference. The percentage of NTI patients with subnormal concentrations of FT4 was 10.1% by UF; 20.3% by mathematical calculation (MC) according to mass-action theory; 19.0% by FT4 index (FT4I), calculated by the triiodothyronine-albumin uptake method expressed as the bound:free ratio; 37.2% by Amerlex M FT4 (P less than 0.001 vs UF, P less than 0.025 vs MC); and 33.3% by Coat-A-Count FT4 with albumin blocker (P less than 0.001 vs UF, P less than 0.025 vs MC). No significant correlations were found between FT4 (UF) and T4-binding proteins (TBP) in serum, but FT4 (MC) correlated with T4-binding globulin (TBG) (r = 0.383, P less than 0.001), FT4I with TBG (r = 0.345, P less than 0.001), FT4 (Amerlex M) with albumin (r = 0.491, P less than 0.001), and FT4 (Coat-A-Count) with TBG (r = 0.636, P less than 0.001). All FT4 measurements correlated well with each other, but correlations were stronger between both calculated FT4 values and FT4 (UF), r = 0.702 to 0.760, than between both values for RIA and FT4 (UF), r = 0.475 to 0.665. These results indicate that in comparison with the UF method, both calculated FT4 values are little affected by TBP concentrations, and FT4 (MC) or FT4I by T3-albumin uptake may be more appropriate than analog RIA for evaluation of circulating FT4 concentration in NTI.

Adult↗
PubMed1988

[UFT + MMC and UFT + ACNU therapy in advanced gastric cancer].

The effects of UFT + MMC and UFT + ACNU therapy on advanced gastric cancer were compared by randomized controlled trial in 12 institutions. Unresectable and postoperatively recurrent patients were divided into PS 0-2 and 3-4 groups. After classification according to cancer spread, such as localized type, hepatic metastatic type, ascitic type and distant metastatic type, regimens of (A) UFT 375 mg/m2/day + MMC 5 mg/m2 for 1-2 W or (B) UFT + ACNU 60 mg/m2/W/x2 (with a 4-week interval) were administered for as long as possible. As a result, among a total of 104 cases, responses were recognized in 7 out of 32 cases (21.9%) treated with regimen A and in 5 out of 25 cases (20.0%) treated with regimen B, giving a total of 57 evaluable cases excluding 33 incompletely evaluated cases and 14 ineligible cases. This study therefore demonstrated no significant difference between the two regimens. The median survival of patients treated with regimen A was 120 days, and that of patients treated with regimen B, 155 days. There was no significant difference between the two regimens. As side effects, UGI symptoms were recognized in 35.4% of regimen A patients and in 37.1% of regimen B patients. Bone marrow suppression appeared in 39.6% of regimen A patients and in 54.3% of regimen B patients.

Adenocarcinoma↗
PubMed1988

Respiratory and metabolic responses of fetal and neonatal perfused livers to catecholamines and anoxia.

The effects of catecholamine and anoxia on the respiratory and metabolic function of developing livers were studied. The characteristic feature of the fetal liver was catecholamine induced glycolysis, although that of the neonatal liver was gluconeogenesis. Infusion of lactate and pyruvate caused a reduction in NAD in the fetus and adult, and NAD oxidation in the newborn. The fetal liver demonstrated marked tolerance of anoxic load, but the tolerance was rapidly lost after birth. The metabolic response to catecholamine may be mediated by the alpha-adrenergic receptor rather than the beta-receptor.

Animals↗
PubMed1988

[Effects of hypobaric-hypoxia on the total number and histochemical properties of the soleus muscle fibers and motoneurons in the rat].

After 7 weeks of hypobaric-hypoxia adaptation, horseradish peroxidase was injected into the soleus muscle to label motoneurons of the spinal cord in rats. Fiber type distribution in the soleus muscle and oxidative enzyme activity of motoneurons innervating the soleus muscle were examined. Fiber type was converted from slow-twitch-oxidative (SO) to fast-twitch-oxidative-glycolytic (FOG). Oxidative enzyme activity of motoneurons (25-45 micron soma diameter) was increased. However, oxidative capacity of larger motoneurons (greater than or equal to 45 micron soma diameter) was not changed. These data suggest that the lack of increase in oxidative capacity of larger motoneurons (innervating SO units) by hypoxia secondarily causes fiber type shift from SO to FOG.

Animals↗
PubMed1987

Metabolic responses to light arm and leg exercise when sitting.

Seven male subjects performed progressive exercises with a light work load on an upper limb or bicycle ergometer in the sitting position. At any comparable work load above zero, arm exercise induced higher oxygen uptake, ventilation, heart rate, oxygen pulse, respiratory rate and tidal volume than leg exercise. At similar levels of VO2 above 0.45 1 X min-1, heart rate and ventilation were higher during arm exercise. A close linear relationship between carbon dioxide output and oxygen uptake was observed during both arm and leg exercises, the slope for arm work being steeper. The ventilatory equivalent for VCO2 (VE/VCO2) gradually decreased during both types of exercise. The ventilatory equivalent for VO2(VE/VO2) remained constant (arm) while it rose (leg) to a peak at 9.8 W and then gradually decreased. Ventilation in relation to tidal volume had a linear relationship with leg exercise, but became curvilinear with arm exercise after tidal volume exceeded 1100 ml. The observed differences in response between arm and leg exercises at a given work load appear to be influenced by differences in sympathetic outflow due to the greater level of static contraction of the relatively small muscle groups required by arm exercise.

Adolescent↗
PubMed1987

Measurements of serum-free thyroid hormone concentrations by ultrafiltration--a comparison with equilibrium dialysis and mathematical calculation.

An ultrafiltration method (UF) for measuring free thyroxine (FT4) and free triiodothyronine (FT3) using the Diaflow YM membrane (Centricon-10) is described. The results are compared with those by equilibrium dialysis (ED) and also by mathematical calculations derived from T4, T3, and binding protein concentrations. The precision with the UF method was excellent. The normal ranges of FT4 and FT3 by the three methods are all comparable. There was a high degree of correlation of FT4 or FT3 results by UF with those by ED and by calculation (r = 0.940-0.974, n = 161, P less than 0.001). FT4 and FT3 by all methods agreed well for hyperthyroidism, hypothyroidism, and for patients with low T4-binding globulin. The mean FT3 in pregnancy was lower than the normal value for all methods, and FT4 concentrations by UF and calculation also decreased in late pregnancy. The mean FT4 by UF and ED in low T3 syndrome were significantly higher than in the normal controls, while the calculated FT4 was lower. The FT3 in low T3 syndrome distributed normal to subnormal in all methods. These results indicate that a) the UF method is a reliable reference method for measuring FT4 and FT3 concentrations; b) the UF results agree well with those by ED and also with theoretically derived values in subjects with thyroid diseases and TBG abnormalities; c) for patients with low T3 syndrome, the FT4 results obtained by UF and ED are similarly discrepant from the calculated results, implying the existence of binding inhibitor(s) which affect both UF and ED measurements.

Adult↗
PubMed1987

[Randomized comparison of continuous and intermittent oral administration of 5'-deoxy-5-fluorouridine in the treatment of advanced gastric cancer; a phase II trial by the Multi-institutional Cooperative Study Group].

To investigate the optimal dosage schedule for 5'-deoxy-5-fluorouridine (5'-DFUR), a randomized comparative study was performed in patients with inoperable or advanced gastric cancer. Eight hundred mg/m2/day of 5'-DFUR was administered in the continuous administration group, while in the intermittent administration group 1,400 mg/m2/day was given for 4 days followed by 3 days with no medication in a one-week cycle, making the total dose 5,600 mg/m2/week in both regimens. In both groups, 5'-DFUR was administered for more than 4 weeks. Overall response rate was 14.3% (3/21) for both administration methods. The response rate for the primary site was 9.5% (2/21) in the continuous administration group, and 14.3% (3/21) in the intermittent group. As to toxicity, the incidence of diarrhea was 26.7% in the continuous group and 4.2% in the intermittent group. Although there was no significant difference between these two dosage schedules in efficacy and toxicity, the lower incidence of diarrhea suggested the clinical usefulness of the intermittent administration method. This result emphasized the need to further studies to investigate the most appropriate dosage schedule for fluorinated pyrimidine derivatives.

Administration, Oral↗
PubMed1986

[Cancer chemotherapy of unresectable primary gastric cancer].

The criteria for cancer chemotherapy of gastric cancers proposed by the Japanese Research Society for Gastric Cancer is introduced with several effective cases. Three subtypes of judgment method--namely, measurable lesions, non-measurable but evaluable by X-ray or endoscopy, and diffuse infiltrating lesions are applied according to the shape and effect of primary foci. Measurable lesions in a case such as aPR should be recorded in metric notation, using a ruler or calipers on X-ray films. bPR is a case when chemotherapy of primary foci is markedly effective with a flattening of the randwall or polypoid part and a shrinkage of cancerous ulceration, but the size of a primary lesion is not decreased. cPR is a case when an expansion of affected site by more than 100% is proved by gastric radiogram or endoscopic image on primary focus in Borrman type 4, a synonym of gastric scirrhous clinically.

Aged↗
PubMed1986

Discrepancy between uptake of serum triiodothyronine as measured by albumin and charcoal methods in nonthyroidal illnesses.

Triiodothyronine (T3) uptake as determined with albumin (AT3U) and with charcoal (CT3U) as binders were compared for patients with low-T3 syndrome. Both T3U values were identical in normal subjects and in patients with thyroidal disorders, pregnancy, low thyroxin-binding globulin (TBG), or acute hepatitis with high concentration of TBG. In the low-T3 syndrome, AT3U was significantly higher than CT3U (38.5 +/- 7.2% vs 29.4 +/- 4.9%, mean +/- SD, n = 37, p less than 0.001), and this difference increased as TBG and albumin concentrations decreased. Decreasing the concentration of TBG and albumin in serum by dilution increased the AT3U more than the CT3U value. Adding albumin to serum decreased AT3U, but not CT3U, when serum TBG and albumin concentrations were low. The free-thyroxin (T4) index as calculated by the AT3U ratio and T4 more accurately reflects the concentration of free T4 in low-T3 syndrome than does the index calculated from the CT3U ratio and T4. Thus AT3U is determined primarily by changes in TBG and albumin concentrations, so that the free-T4 index based on the AT3U ratio and T4 may be more useful than the CT3U method for evaluating free-T4 concentrations in low-T3 syndrome, which frequently has concomitant decreases in TBG and albumin concentrations.

Adult↗
PubMed1986

[Fundamental and clinical studies of imipenem/cilastatin sodium in the field of obstetrics and gynecology].

A new carbapenem antibiotic and renal dipeptidase inhibitor, imipenem/cilastatin sodium (MK-0787/MK-0791) were studied for the distribution into various tissues and for clinical aspects in obstetrical and gynecological fields. The following results were obtained. Concentrations of the drug in arterial blood, venous blood, and internal genital organs following an intravenous drip infusion were measured. The results demonstrated good transfer of the drug into various internal genital organs. Four patients with bacterial infections were treated with MK-0787/MK-0791. Therapeutic results were good in 2 cases for, an effective rate of 50%. No side effects were noted in any cases. The above results suggested that MK-0787/MK-0791 may be a useful antibiotic for infectious diseases in the field of obstetrics and gynecology.

Adult↗