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Biomedical subjects

S Tanida

Publications and source records attributed to S Tanida.

At least 37 records · Page 2Linked to original sources
PubMed1989

TAN-999 and TAN-1030A, new indolocarbazole alkaloids with macrophage-activating properties.

Two new indolocarbazole alkaloids, TAN-999 and TAN-1030A, were isolated from culture broths of Nocardiopsis dassonvillei C-71425 and Streptomyces sp. C-71799, respectively. Their structures were elucidated on the basis of their reactions, spectroscopic analyses and in particular, comparison of spectral data with that of staurosporine. These metabolites induced spreading of a murine macrophage cell line, Mm 1. They also augmented the phagocytic activity, Fc gamma receptor expression and beta-glucuronidase activity of murine macrophage cell lines, Mm 1 and J774A.1. When proteose-peptone elicited peritoneal macrophages from mice were incubated with these metabolites for 2 days, the phagocytosis-dependent respiratory burst of these cells was enhanced. Similar enhancement was also observed when the peritoneal macrophages in mice were modulated by intraperitoneal administration of these metabolites. These results reveal that TAN-999 and TAN-1030A can activate macrophage functions in mice.

Alkaloids↗
PubMed1987

A new pyrrole-amidine antibiotic TAN-868 A.

A new pyrrole-amidine antibiotic TAN-868 A was isolated from the culture broth of Streptomyces idiomorphus sp. nov. Its chemical structure was determined by spectroscopic analyses and degradation studies to be 4-[(2S,4R)-4-hydroxy-5-iminoprolyl]amino- N-(2-amidinoethenyl)-2-pyrrolecarboxamide. The antibiotic is active against bacteria, fungi and a protozoan, and has cytotoxic activity against murine tumor cells. DNA thermal denaturation studies suggest that TAN-868 A preferentially interacts with AT rich regions of double-stranded DNA.

Anti-Bacterial Agents↗
PubMed1986

Two sulfur-containing ansamycin antibiotics from Streptomyces albolongus.

Two sulfur-containing ansamycin antibiotics were isolated from the culture broth of Streptomyces albolongus C-46366; the major one was identical with awamycin and the minor one was a new ansamycin antibiotic, ansathiazin. Their structures were elucidated from their reactions and spectroscopic analyses. These antibiotics were active against gram-positive bacteria, acid-fast bacteria and a protozoan.

Anti-Bacterial Agents↗
PubMed1982

Mechanism of action of dnacin B1, a new benzoquinoid antibiotic with antitumor properties.

Dnacin B1 preferentially inhibited the incorporation of [3H]thymidine into acid-insoluble fractions in Escherichia coli. At a sublethal concentration, dnacin B1 caused filamentous growth in E. coli and induced prophage lambda. The antibiotic also showed potent bactericidal activity against repair-deficient E. coli strains, such as recA, recB, and polA strains, In in vitro studies, dnacin Ba raised the melting temperatures of various double-stranded DNAs. In addition, the antibiotic showed DNA-cleaving activity against PM2 DNA in the presence of reducing agents, and the activity was suppressed by scavengers for oxygen free radicals and an iron-specific chelator, desferrioxamine E. The stimulation of the generation of superoxide radical by dnacin B1 was confirmed by measuring the reduction of neotetrazolium. Therefore, it can be presumed that the primary cellular target of dnacin B1 is DNA in susceptible cells, and the autooxidation of DNA-bound dnacin B1 causes the generation of oxygen-free radicals that result in the damage of DNA and the inhibition of its synthesis.

Antibiotics, Antineoplastic↗
PubMed1981

Ansamitocin analogs from a mutant strain of Nocardia. I. Isolation of the mutant, fermentation and antimicrobial properties.

A mutant having a high ability to produce ansamitocins was derived from a dnacin-producing strain, Nocardia sp. No. C-14482 (N-1001), by treatment with ethidium bromide. Mutant N-1231 produced ansamitocins P-3 and P-4 as major components, but was deficient in its ability to produce dnacins. Strain N-1231 also produced fifteen novel ansamitocin analogs as minor components. These analogs showed no activity against prokaryotic micro-organisms. The results of determining the activity inhibiting cilia regeneration of deciliated Tetrahymena pyriformis suggest that hydroxylation of C15, C26 and the acyl moiety at C3 of ansamitocins may cause marked reduction of their antitubulinic activities whereas demethylation of -NCH3 at C18 slightly affected their activities.

Anti-Bacterial Agents↗
PubMed1980

Ansamitocin-induced synchrony in Tetrahymena pyriformis.

Ansamitocins inhibited cell division in Tetrahymena pyriformis W at low concentrations and caused cells to become more rounded. Exposure to ansamitocins for 5 h or more resulted in a burst of synchronous division at 100 min after removal of the antibiotics with a maximum division index of 70%. A second burst of synchronous division occurred 300 min after removal with a peak division index of 55%. The rounding of the cell shape was restored by the completion of the first division. When RNA or protein synthesis was blocked by chromomycin A3 or cycloheximide, the first division was suppressed. When DNA synthesis was blocked by methotrexate plus uridine, synchronous division still occurred; furthermore, the DNA content of T. pyriformis cells did not increase before the first division. These findings suggests that the first synchronous division requires RNA and protein synthesis but does not require DNA synthesis. Interference by ansamitocins with the fuction of microtubule systems in T. pyriformis is discussed.

Animals↗
PubMed1980

Dnacins, new antibiotics. I. Producing organism, fermentation, and antimicrobial activities.

Dnacins are new antibiotics produced by an actinomycete, strain No. C-14482 (N-1001). The characteristic features of the organism are: the formation of coremia on solid media, production of rod-shaped motile spores with peritrichous flagella from mature aerial mycella, fragmentation of the mature organism (at later stage of growth) in liquid media in which some fragmented elements have motility, lysozyme resistance, meso-diaminopimelic acid in the cell wall, and a guanine-cytosine content of 71 +/- 1 mol%. The organism has been designated as Nocardia sp. No. C-14482 (N-1001). Dnacins show strong activity against various GRam-negative, Gram-positive, and acid-fast bacteria, but slight activity against fungi. The antibiotics hardly affect the growth of Escherichia coli K-12 under anaerobic condition even at concentrations more than five times that of the minimum inhibitory concentrations under aerobic conditions.

Anti-Bacterial Agents↗
PubMed1980

Dnacins, new antibiotics II. Isolation and characterization.

Dnacins A1 and B1, new basic antibiotics with strong and broad antibacterial activities, were isolated as dark red or reddish brown needles from the culture broth of Nocardia sp. No. C-14482. The characteristic absorption maxima at 213 nm, 281 or 283 nm and 496 nm in the UV and visible range and other physicochemical properties indicated that dnacins A1 and B1 are novel antibiotics which belong to the group having aminobenzoquinone moieties.

Anti-Bacterial Agents↗
PubMed1980

Macbecins I and II, new antitumor antibiotics. I. Producing organism, fermentation and antimicrobial activities.

New antibiotics, macbecins I and II, have been found in the culture fluid of an actinomycete, which has the following properties: delayed fragmentation of vegetative mycelia, formation of coremia on solid media, the occurrence of meso-diaminopimelic acid in the cell wall, lysozyme resistance, and guanine-cytosine content of 71 +/- 1 mol%. The organism has been designated Nocardia sp. No. C-14919 (N-2001). A marked enhancement of the production of macbecins I and II was observed in cultures containing L-tyrosine. The antibiotics are moderately active against several Gram-positive bacteria and fungi. The antibiotics also inhibit the growth of Tetrahymena pyriformis W at 2 microgram/ml but show no activity against the regeneration of cilia in partially deciliated Tetrahymena at 10 microgram/ml.

Amino Acids↗
PubMed1976

Inhibition of coliphage multiplication and R plasmid transfer by desdanine.

Desdanine inhibited the plaque formation of male-specific coliphages but not that of other coliphages tested. Desdanine also suppressed the multiplication of both RNA phage Q beta and filamentous DNA phage f1 at the concentration of 3.13 approximately 6.25 mug/ml which had no influence on the growth of their host cells. However, the inhibitory effect on the phage multiplication was not due to the inactivation of phage particles nor the prevention of phage adsorption and penetration into the host cells. Desdanine also inhibited the transfer of R plasmid, R 100-1, in E. coli at 6.25 approximately 12.5 mug/ml without affecting the viability of donor and recipient cells.

Acrylamides↗