Biomedical subjects

S Teleman

Publications and source records attributed to S Teleman.

15 recordsLinked to original sources

PubMedDate not supplied

Histological study of Hodgkin disease (HD), an immunohistochemical identification of the cells surrounding Sternberg-Reed cells.

In this study, we analyze histological types of 77 cases H.D. and examine immunohistochemically the reactive cells surrounding Reed-Sternberg cells (40 cases). On the current techniques sections of lymph nodes were def ned H.D. types after the new criteria (Bennett & col., 1985, Buttler, 1992). Immunologic marker studies were performed on paraffin sections of H.D. tissue utilizing the avidin--biotin peroxidase complex technique with limited panel of monoclonal antibodies OKT3, OKB1, OKM1. On histopathologic grounds we established the diagnosis of lymphocytic predominance type in 6 cases--8%, nodular sclerosis type in 36 cases--47%, from which first degree--24 cases and second degree--12 cases, mixed cellularity type in 28 cases--36% and lymphocytic depletion type in 7 cases--9%. Immunohistochemically, in our 40 H.D. cases, the number of T. lymphocytes in all H.D. types, was highly variable, ranging from a minority to a vast majority of cells directly surrounding the Reed-Sternberg cells. These lymphocytes were positive for OKT3, which were more in tissue involved by nodular sclerosis or mixed cellularity types than in the lymphocytic predominance type. Thus, in our few cases of lymphocytic predominance type, the reactive proliferation was consisted of B. cells along side with a few T cells. Also the OKM1 positive mononuclear cells were few. In conclusion, our data support the necessity to extend of several immunohistochemical investigations used for distinguishing H.D. disease from benign hyperplasia and from non-lymphoid malignancies, and for concerning the functional lymphocytic composition and the role of monocyte-macrophage system in the immunological alteration in H.D.

Adolescent↗

PubMedDate not supplied

[An immunohistochemical study of the steroid hormone receptors in endometrial hyperplasia].

AIM: Evaluation of the estrogen and progesterone receptors (ER and PR) in different degrees of endometrial hyperplasias. METHODS: 10 cases of each degree of endometrial hyperplasia (simple, complex and atypical) were analyzed using the avidin-biotin-peroxidase technique and monoclonal antibodies applied to formalin-fixed, paraffin-embedded tissue. RESULTS: We found similar, high level of both ER and PR in simple and complex hyperplasias and a significant decrease of these in atypical hyperplasia. CONCLUSIONS: Endometrial hyperplasias develop in a setting of estrogen excess. This explains the high level of endometrial receptors and the response to progesterone in typical hyperplasia. In atypical hyperplasia, the decrease of steroid receptors results in a low sensibility of this lesion to progestative therapy, but there are cases with high receptor levels which could have a chance for hormonal therapy.

Biomarkers↗

PubMedDate not supplied

[p53 and steroid receptors support double endometrial carcinogenesis].

AIM: This study investigated the p53 positivity correlated with the receptor status, in different grades and stages of endometrial carcinoma. METHODS: The study included 88 endometrioid-type carcinomas and 5 non-endometrioid-type carcinomas. Paraffin sections were used for the ordinary histological diagnosis and for immunohistochemical diagnosis by avidin-biotin-peroxidase technique. RESULTS: p53 positivity was identified in 10 endometrioid-type carcinomas (11.4%) and 3 non-endometrioid type carcinomas (60%). Most of the p53 positive cases were receptor negative and observed in advanced stages and histological grades. No associated endometrial hyperplasia was p53 positive, while a positive intraepithelial non-endometrioid carcinoma was identified. CONCLUSIONS: p53 positivity is most frequently identified in non-endometrioid type, receptor negative adenocarcinomas, even in a precursor lesion as it is intraepithelial carcinoma, while in endometrioid, receptor positive carcinomas it appear in late stages of development, never being identified in precursor lesions.

Carcinoma↗

PubMedDate not supplied

[Endometrial hyperplasia predicts a better prognosis in endometrial carcinoma].

AIM: To evaluate the significance of the associated Endometrial Hyperplasia in Endometrial Carcinoma. STUDY DESIGN: 93 hysterectomy specimens for endometrial carcinoma were histologically reviewed on paraffin sections stained H&E and tricromic VG. RESULTS: 45 cases were associated with endometrial hyperplasia; 48 cases without endometrial hyperplasia were: 35 cases with the entire endometrial cavity occupied by carcinoma, 11 associated with endometrial atrophy and 2 with normal proliferative endometrium. In this second group, 37 cases (77%) were in advanced stages. A correlation was found between the presence of Endometrial Hyperplasia and the degree of differentiation, the FIGO stage of the carcinoma, and the age of the patient. CONCLUSIONS: Endometrial Carcinomas associated with Endometrial Hyperplasia involve younger women and have lower aggressivity than those without hyperplasia. Endometrial Hyperplasia may demonstrate a favorable prognosis in Endometrial Carcinomas.

Carcinoma, Endometrioid↗

PubMedDate not supplied

[Morphological correlations between endometrial hyperplasias, uterine leiomyoma and ovarian associated lesions].

Morphological evaluation and correlation of endometrial hyperplasias, leiomyoma and ovarian estrogen producing lesions. 390 specimens of hysterectomy associated or not with ovarectomy were processed by routine technique and sections stained by H&E staining. 316 cases presented different degrees of endometrial hyperplasias associated with leiomyoma. In 117 from 170 cases the ovary presented estrogen secreting lesions (follicular cyst, polycystic ovary, stromal hyperplasia, granulosa cell tumours). The highest frequency of the uterine and ovarian lesions was in the decades 41-50 years, and 31-40 years. Leiomyoma and endometrial hyperplasia, develop in a hormonal context, most frequently characterized by micropolicystic ovaries. The risk is higher in the perimenopausal period. The most frequent type is simple hyperplasia suggesting a rare progression to highest grades and a possible protective role of leiomyoma as target tissue which capture estrogens.

Adult↗

PubMedDate not supplied

[Value of steroid receptors assessment in atypical endometrial hyperplasia].

To evaluate if the immunohistochemical assessment of steroid receptors is helpful in distinguishing Atypical Hyperplasia from Well Differentiated Endometrial Carcinoma, the morphological and immunohistochemical features for steroid receptors were studied in 46 cases of atypical hyperplasia alone or associated with other degrees of hyperplasia or adenocarcinoma. The estrogen and progesterone receptors were determined by the avidin-biotin complex technique using monoclonal antibodies on paraffin embedded tissue. The diagnosis of atypical hyperplasia was based on the observation of epithelial atypias and the absence of the stromal invasion. The positive reaction for steroid receptors was moderate, very variable from case to case, heterogeneous and quite similar to that observed in carcinoma. The morphological identification of the stromal invasion is the most reliable criterion in differentiating endometrial carcinoma from atypical hyperplasia. The steroid receptors assessment is not valuable in this purpose but may be useful in cases in which a hormonal therapy is recommended.

Adenocarcinoma↗

PubMedDate not supplied

[The prognostic molecular factors in precancerous lesions and nonmelanotic skin carcinoma].

The most common cutaneous carcinoma, which includes basal cell carcinoma (BCC) and squamous cell carcinomas (SCC), accounts for approximately 90% or more of all skin malignancies. Actinic keratosis, bowenoid type of actinic keratosis, Bowen's disease or SCC in situ have been considered precursors of skin SCC. Authors present the histopathological criteria of precursors of skin SCC, variants invasive SCC and BCC. Also, one discusses about the roles of immunohistochemical staining of gene products in evidence of molecular changes in cell that might occur according to the transformation from precancerous lesions to SCC.

Bowen's Disease↗

PubMedDate not supplied

[Medical and social implication of cervical cancer screening].

Invasive cervical cancer represents a major health problem in Romania. When there is a functioning screening network, the incidence of invasive lesions has a marked reduction, this program being the most successful cancer prevention program of all times. 80% of the actual incidence and mortality from this disease occurs in countries without an organized screening. A good infrastructure of the cytopathologist--practitioner relation must be achieved in order to reach the standard of early detection of the preinvasive cervical lesions.

Female↗

PubMedDate not supplied

[Malignant mixed mesodermal tumor: histo- and cytopathologic correlations].

To correlate the cytopathological and the histopathological findings in uterine mixed mesodermal malignant tumor (MMMT) we have examined the cervical smear, endometrial curettage and hysterectomy specimen of a patient diagnosed with uterine tumor. The smear was stained by Papanicolaou staining and the tissue processed by routine technique and stained H&E. The original cytological diagnosis was adenosquamous carcinoma. The histopathological diagnosis was MMMT of heterologous type. A review of the smear revealed features which may orientate the diagnosis: multinucleate cells, isolated cells with cyanophilic cytoplasm, hyperchromatic nuclei and prominent nucleoli, elongated cyanophilic cells of sarcomatous origin. We conclude that the cytopathological diagnosis of the MMMT in cervical smears is very difficult. This may be sustained by the evidence of more cell types and cellular features orientating to a sarcomatous origin. The most important differential diagnosis is adenosquamous carcinoma.

Adenocarcinoma↗

PubMedDate not supplied

[Endometrial carcinoma in situ. Morphological and immunohistochemical aspects].

A possible precursor for invasive endometrial carcinoma is an in situ stage, but this notion is very much debated. We present a case of endometrial carcinoma diagnosed in a patient with post-menopausal bleeding. The morphological examination of the hysterectomy specimen, processed by routine technique, put in evidence focal atypias of the glandular epithelium in a group of few endometrial glands, on a background of atrophy without a desmoplastic stromal reaction. The immunohistochemical investigation of the steroid receptors and p53 protein, using monoclonal antibodies in the ABC technique, revealed the p53 positivity and the absence of the receptors in the focal malignant areas. The surface epithelium had the same immunohistochemical aspect. These aspects were decisive in precising that the malignant change was primarily in the surface epithelium with secondary extension in some glands, the final diagnosis being in situ serous papillary carcinoma.

Aged↗

PubMedDate not supplied

[Diagnosis of gastric cancer on endobiopsies using the WHO classification].

Gastric cancer (GC), one of the most frequent malignancies can be early detected on endobiopsies. Our aim was to evaluate histologically the GC on endobiopsies, using WHO 2000 and Lauren classifications. The study included 2424 gastric endobiopsies, routine processed; sections stained with HE, Giemsa, PAS and AB. GC was diagnosed in 451 cases (19%), mostly in men (311 cases--69%). The highest incidence was in 60-69 year-old aged patients (34%). Using Lauren classification, 279 cases were included in diffuse type (62%), 167 intestinal-type (37%) and 5 (1%) mixed type. Each of these three types were also histologically analyzed considering the WHO classification. We pointed out that GC can be diagnosed histologically on endobiopsy specimens, although it is difficult to diagnose the GC--mixt type, due to reduced size of endobiopsies. Lauren classification (including the two major types: diffuse and intestinal) is very useful, especially if correlated with histological criteria of WHO classification.

Adenocarcinoma↗

PubMedDate not supplied

[Preclinical assay on a magnetic carrier type ferrofluid].

UNLABELLED: The aim of the study was to determine the acute toxicity and the antimicrobial actions of an original magnetic carrier, type ferrofluid. The hydrophilic ferrofluid was prepared by covering the Fe3O4 nannoparticles with ammoniumoleate. The absolute amount of iron was of 40 mg/ml ferrofluid. METHODS: Acute toxicity was evaluated on five groups of Swiss male mice, after a single intraperitoneal administration of 1, 0.75, 0.5, 0.25 and 0.125 ml dose of pure ferrofluid/100 g body weight (b.w.), using step-level toxicity method. The study groups of mice were follow-up for 10 days. We did not use the same volume of solution for all the study groups because we were concerned about not to modify the behavior of the ferrofluid (but for each group we used the same volume of solution). The tasks of this part of the study were: 1) the record of the mice death in the first 10 days after intraperitoneal administration of ferrofluid; 2) the behavior of the animal subjects; 3) the morphopathologic examination of kidney, lung, heart, liver and peritoneum samples from the death mice and from the after ten days survivors which were sacrificed. We also investigated the possible antibacterial actions of the ferrofluid on E. coli spp., Klebsiella spp., Staphylococcus aureus Streptococcus group D., in the second part of the study, using standard lab kit. The validation of the results was performed using controls for E. colli and Staphylococcus aureus. RESULTS: The death of the mice was registered between 24 and finished after 96 hours. The maximum tolerated dose (MTD), was of 0.25 ml (10 mg iron/100 g b.w.) and the lethal dose hundred percent (LD100) was of 0.75 ml/(30 mg iron/100 b.w.). In our study we did not determined any kind of antibacterial action of the ferrofluid. CONCLUSIONS: 1) LD100, in our study, was of 30 mg iron/100 g b.w., and DMT of 10 mg iron/100 g b.w. 2) The death of the mice may be due to toxic aggression of ammonium ions released, in vivo, from the ammoniumoleate coverage of magnetite nannoparticles. 3) There were no in vitro antibacterial actions for this ferrofluid.

Animals↗

PubMedDate not supplied

[ASCUS of metaplastic type. Cyto-histopathological correlations].

We evaluated the cytopathological changes and the prognostic significance of atypical squamous metaplastic cells in cervical smears. 50 ASCUS cases were divided in two groups: 25 cases in different settings and 25 of metaplastic type. All cases were re-evaluated after 6 months and when necessary, verified by biopsy. The second cytological diagnosis was: group I--13 normal, 2 LSIL, 40 ASCUS; group II--6 normal, 2 LSIL, 2 HSIL and 15 ASCUS. The HPV was positive in 5 cases in group I and in 9 cases in group II. In ASCUS persistent cases the biopsy revealed: in group I--3 CIN 1 and 1 CIN 2 cases; in group II--1 CIN 1, 2 CIN II, 2 CIN III and one case of microinvasive carcinoma. In conclusion, atypical metaplastic cells are more frequently involved in serious cervical lesions.

Adult↗

PubMedDate not supplied

[Cervugid ovules in cervico-vaginal infections and cervix uteri precancerous conditions treatment].

UNLABELLED: This medicine was authorized by the National Drug Agency (ANM, Bucureşti) in 2001. PURPOSE: To evaluate the effectiveness and the tolerance to Cervugid-ovules, a preparation that combines the polyvalent local antiinflammatory action of chloramphenicol, metronidazole and nystatin with the effect of hydrocortisone acetate, an unspecific anti-inflammatory agent; they all are embedded in a Lipex-403, semisynthetic fat. MATERIALS AND METHODS: The evaluation of 500 patients ages between 15 and 85 years with genital infections, registered in the files of "Cl. II Obst. and Gynecology" of the Cuza-Vodă Hospital from Iaşi has been studied. We studied the subjective manifestations (local discomfort and pelvic pains, local burning and dryness,vulvovaginal itching and dyspareunia) and objective manifestations (vaginal and cervical secretion, the cytotest performed and colored though the Papanicolaou method and reported in the Bethesda system). RESULTS AND DISCUSSION: Healing of the subjective symptoms in 98%, healing of the leukorrhea--as a main objective symptom--in 95%; The Bethesda system cytotest was one of the inflammatory type in the most of the cases and there wew found in 85 cases: 6 ASCUS, 41 LSIL, and 37 HSIL. The use of Cervugid had a healing response in most of the cases when used in acute and chronic cervico-vaginal inflammatory processes. Cervugid may be considered as an important agent in the treatment of the precancerous affections af the cervix uteri on the following reasons: zhe cure of the infections caused by chlamydia, involved in the etiology of cervical neoplasms, the cure of the HPV infection under episome form, classified in the Bethesda system within the ASCUS, AGUS or LSIL classes. When the cytotest was in the HSIL class, a conization in the LLETZ method was performed. CONCLUSIONS: Cervugid is conceived for those three main categories of pathogenic factors related to the etiology of cervico-vaginitis: microbia germs, protozoa and mycosis. In addition, it is active on chlamydia and mycoplasms, always sensitive to chloramphenicol therapy. That is why Cervugid with in local administration is indicated in the microbial, trichomoniasis and mycotic vaginitis caused by one category of pathogenic agents or by associated forms, in cervicitis, in the pelvic inflammatory processes (pelvic congestion, metritis, adnexitis, and inflammatory processes associated with benign or malignant tumors of the genital apparatus). The results obtained proved that Cervugid is highly effective medicine.

Administration, Intravaginal↗

PubMedDate not supplied

[Anal squamous cell carcinoma synchronous with rectal adenocarcinoma].

Most often colorectal carcinoma occurs single; synchronous multiple carcinomas usually develop at widely disparate sites. We report the case of a 75-year-old male, accusing rectal bleeding, disturbances in bowel transit and weight loss. The rectoscopy examination revealed a fungating, bleeding tumor located 5 cm from anal verge. Pathological diagnostic of the endo-biopsy was ulcerated moderate differentiated adenocarcinoma. Patient underwent surgical amputation of the rectum with lymphadenectomy. Microscopical examination of the surgical specimens confirmed the presence of the adenocarcinoma adjacent to a squamous cell carcinoma, moderate differentiated, with reduced keratinization, infiltrative. Also, 2 from the 7 lymph nodes presented squamous cell carcinoma metastases. The most important differential diagnostic is a rectal adenosquamous carcinoma. Prognostic depends on stage of the disease, generally being worse than of the corresponding adenocarcinoma, and can be improved by radio- and chemotherapy.

Adenocarcinoma↗