PubMed Health⌕ Search

Biomedical subjects

S Toida

Publications and source records attributed to S Toida.

17 recordsLinked to original sources
PubMed2000

Acinar cell carcinoma of the pancreas showing finger-print-like zymogen granules by electron microscopy: immunohistochemical study.

A rare case of finger-print-like zymogen granules shown by electron microscopy is reported. The patient was a 75-year-old man who was histologically and ultrastructurally confirmed to have acinar cell carcinoma of the pancreas. Frozen section and postmortem examination revealed that the tumor was made up of solid nests of cells resembling the appearance of normal pancreatic acini, showing polygonal cells which had round or oval nuclei, and rare mitotic figures. Zymogen-like granules, shown by eosinophilic granular staining, were abundant in the cytoplasm. Electron microscopy showed that the tumor cells were closely packed, occasionally forming small intercellular spaces resembling pancreatic acini (microtubules). The cytoplasm contained characteristic zymogen granules with dark-to-medium electron density, measuring 660 nm +/-213 SD in diameter. The granules of medium density were large, and showed finger-print-like patterns. Investigation of more cases is necessary to identify whether these finger-print-like patterns are an important factor in the genesis of acinar cell carcinoma.

Aged↗
PubMed1999

[Possibility of discharge and need for rehabilitation of psychiatric patients hospitalized for one year or more in Japan: a preliminary report. Committee on Rehabilitation Affairs, Subcommittee of Survey on Rehabilitation Need].

A total of 19,342 psychiatric patients staying in a total of 143 hospitals in Japan for one year or more entered in this study in order to determine the possibility of discharge (POD) and the need for rehabilitation. Those who were assessed by the psychiatrist in charge to have POD provided community support was assured accounted for 32.5%. As for the levels of daily life functions measured with the GAF score, those assessed to have POD showed the maximum frequency between scores 51 and 60, while the others, who were not considered suitable for discharge, showed the maximum frequency between scores 21 and 30. On the other hand, the control group, consisting of subjects with psychiatric disabilities and living in the community while using day care or rehabilitation facilities, showed the maximum frequency of GAF scores between 51 and 60. Two-thirds of the study subjects were older than fifty, while in the control group those aged between 30 and 49 accounted for 49.0%, thus indicating that the residents of mental hospitals tend to be older. More than 60% of the study subjects had been staying in hospital for five years or more. Those without their own home accounted for about 60%.

Adult↗
PubMed1996

Effect of high sucrose feeding on fat accumulation in the male Wistar rat.

High sucrose intake is generally thought to be a risk factor for obesity and insulin resistance. We examined the effects of feeding sucrose on fat accumulation and insulin release in male rats. Six-week-old male Wistar rats were maintained on a high sucrose diet for 4 or 12 weeks. Control rats were fed a diet based on starch. No significant difference in daily caloric intake or weight gain existed between the two dietary groups. There was no difference between the two dietary groups in the gain of abdominal subcutaneous fat (SC) at 4-week. In contrast, rats fed the high sucrose diet had significantly more mesenteric fat (MES) than controls (p < 0.01). At 12 weeks, rats fed the high sucrose diet had significantly more SC and MES than controls (SC: p < 0.05, MES: p < 0.01). Basal immunoreactive insulin (IRI) concentrations in the portal vein (PV) of rats fed the high sucrose diet was significantly higher compared to those of controls (4 wk: p < 0.05, 12 wk: p < 0.05). No difference between the two dietary groups in basal IRI concentrations in the inferior vena cava (IVC) existed at 4 weeks; whereas at 12 weeks, the basal IRI concentrations in the IVC in rats fed the high sucrose diet were significantly higher than in controls (p < 0.05). The mesenteric and subcutaneous fat accumulations were closely related to hyperinsulinemia in the portal vein and inferior vena cava, respectively. Twelve weeks of high sucrose feeding caused accumulation of abdominal adipose tissue with marked hyperinsulinemia and hyperlipidemia. Our study is the first to demonstrate that abdominal fat induced by high sucrose intake in male rats is accompanied by an abnormal metabolic state similar to an insulin-resistant state.

Abdomen↗
PubMed1986

Application of pop-off method to bone marrow and peripheral blood specimens for purposes of electron microscopy.

In this paper, we describe a pop-off method applicable to the hematological field. Bone marrow or peripheral blood specimens from patients were placed on a clean glass slide and fixed immediately in 2% glutaraldehyde solution for 10 minutes. For the DAB reaction, the slide was immersed in the DAB reagent for 30 minutes, and post fixed with 1% OsO4 solution for 1 hour. Specimens on the slide were washed with buffer solution, dehydrated and polymerized directly on the slide. A gelatin capsule filled with Epon mixed monomer was then reversed over the specimen. After polymerization was completed, the specimen was popped off from the slide to the capsule and trimmed carefully to prepare for ultrathin sectioning. This method allows the entire sequence of tissue preparation to be carried out on the slide, from fixation to embedding, and even, especially in scanty specimens, including the DAB reaction. Electron microscopic findings in specimens prepared by this technique show excellent preservation and the absence of specific artifacts.

Blood Cells↗
PubMed1980

[Comparative study on rat arthritis induced by various immune mechanisms (author's transl)].

Experimental arthritis was induced in rats by the use of horse ferritin as an antigen. The pathological findings were studied and the mechanisms of inflammation were analyzed. Arthritis was induced through various mode of actions: 1. Arthus reaction (antigen-induced arthritis). 2. Passive Arthus reaction. 3. Reverse passive Arthus reaction. 4. Arthritis induced by intra-articular injection of immune complex, by either single shot or repeated injections. 5. Arthritis induced by anamnestic reaction. Arthritis induced by repeated injections of immune complex or that induced by anamnestic reaction exhibited mild chronic synovitis characterized by round cell infiltration without detectable acute phase of inflammation. Arthritis induced through other immunological mechanisms revealed a sequential course of acute synovitis characterized by accumulation of polymorphonuclear leucocytes (PMN) followed by gradual disappearance of PMN and alternative increase of round cells or plasma cells and proliferation of synovial lining cells. All types of arthritis subsided with mild fibrosis of the synovial tissue without lasting over a significantly long time. Although the successful production of a long-lasting chronic arthritis has been reported in rabbits, it was impossible to induce such long standing arthritis through this experiment. The chronicity of the arthritis induced through immunological mechanism may not simply be due to the long-standing retention of immune complex within the involved joint but also due to other factors such as the species of the test animals, the nature of antigen used and local factors produced through acute joint inflammation.

Animals↗
PubMed1980

[Studies on the immuno-suppressive effects of adriamycin and tumor metastasis (author's transl)].

Most of the antineoplastic agents were known to have immuno-suppressive effect. The purpose of the present work is to test the immuno-suppressive effect of adriamycin, an effective and widely used chemotherapeutic agent in the field of clinical orthopedics. Isogenic ACI rats and their transplantable fibrosarcoma AMC-60 were used as test materials. Tumor cells were injected as an artificial model for pulmonary metastasis or inoculated into the tibial bone to examine any metastatic focus other than the inoculated site. Administration of one course of adriamycin in the same way as that was given in patients induced temporarily leucopenia with prominent decrease of granulocytes. Adriamycin suppressed humoral antibody production to sheep erythrocytes in the treated rats and also suppressed cellular immune response expressed by skin reaction to PHA in the treated rats. Administration of adriamycin one week before tumor inoculation into immune rats which have acquired immunity to AMC-60 through preimmunization with the tumor, induced increased incidence of pulmonary metastasis, whereas administration of adriamycin after tumor inoculation resulted in decreased incidence of metastasis or healing of the inoculated tumor. Inoculation of bone marrow cells from healthy rats restored the impaired immunity. In unimmunized rats, pretreatment by adriamycin resulted in accelerated tumor growth at the inoculated site and increased incidence of metastasis. On the other hand, adriamycin given after tumor inoculation suppressed tumor growth and repeated administration of adriamycin induced healing of the inoculated tumor. The present data indicates that even an effective antineoplastic agent will interfere with the immune surveillance system of the host and result in accelerated tumor growth or increased incidence of metastasis when improperly given. A continual efforts to search for a less immuno-suppressive and effective method of administration of a given antineoplastic agent is necessary.

Animals↗
PubMedDate not supplied

Histopathological and immunohistochemical studies of acquired tracheobronchomalacia: an autopsy case report.

A 72-year-old patient was reported to have 'saber-sheath-type' acquired tracheobronchomalacia after irradiation therapy for laryngeal cancer. The pathogenetic features of this case were demonstrated immunohistopathologically using CD68, HLA-DR, UCHL1, L26 and Ki-67 antibodies. The principal features were (a) selective destruction of the cartilage with Ki-67 stainability from the trachea to the segmental bronchi with inflammatory infiltrations of predominant T lymphocytes (UCHL1-positive) and activated macrophages (CD68-positive with marked HLA class II antigen) and (b) replacement by collagen fibers through the affected lesion. The tissues, except for the cartilage in the airway tract, were preserved without marked change, including the membranous portion. The cartilage at organs other than the airway showed no changes. We first clarified the immunohistopathology of tracheobronchomalacia. We proposed that the characteristic cell-mediated immunity against cartilage with T lymphocytes and activated macrophages in the pathogenetic features may be related to a cancer-healing tendency.

Aged↗