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Biomedical subjects

S Toivonen

Publications and source records attributed to S Toivonen.

At least 19 recordsLinked to original sources

A study of sleep patterns on two Finnish icebreakers, ambulatory recording and automatic analysis.

The effects of noise and vibration on sleep in Finnish ice-breaking ships were studied in healthy volunteer workers and a control group. EEG, EOG and EMG were recorded by means of portable tape recorders. The recordings were analysed by an automatic hybrid system. Both sleep stage parameters and the quantities of single EEG waveforms were used for the evaluation of the sleep quality. Measurements were made before, during and after the ice-breaking season in the winter. Higher amounts of wakefulness and sleep stage 1, and lower amounts of delta and theta activity were found on a night during the ice-breaking season, compared with a night after the season. This was interpreted as indicating a "lightening" of sleep, caused by noise and vibration. No differences between the nights before and during the ice-breaking season were observed. The crew members had an appr. 1 h shorter Time In Bed and Sleep Period Time than the controls also on the nights studied ashore. Because of the small number of subjects the results are not conclusive, even though statistically significant differences were obtained. The delta activity in seconds/minute seems to be a more informative measure than the percentages of the sleep stages S3 and S4. The possibility of using the theta activity as a measure of the length of sleep should be further investigated.

Adult

The surface charge of mesodermal and neural cells of Triturus embryos.

The electrical surface charge of disaggregated mesodermal and neural cells of the neurula stage of Triturus vulgaris embryos is estimated by using the apparatus described in Figure 1. The results showed a significant difference between the net charges of these two cell types: the neural cells had a clearly negative charge, whereas the mesodermal cells seemed to be more or less neutral. The difference of the surface charges may partly explain the "sorting-out" phenomenon in the mixed reaggregates of the disaggregated cells.

Animals

Mechanisms of cell interaction during primary embryonic induction studied in transfilter experiments.

The transmission mechanisms operative at different stages of neutralisation during primary embryonic induction of the newt Triturus vulgaris were studied in experiments employing Nuclepore filters placed between interactive tissue explants. The transmission time of the neuralising effect was determined with 0.2 mum Nuclepore filter. In another series of experiments the transformation of neuralised ectoderm by archenteron roof mesoderm into other parts of the CNS was studied. Although sufficiently long induction times were used no transformation into hindbrain structures could be induced across filters with pore sizes from 0.1 mum to 1.0 mum. However, electron microscopy demonstrated cytoplasmic penetration into 0.6 mum filters at 15 h of induction. The results speak against free long-range diffusion of inductive material at the stage of transformation of the neuralised ectoderm to more caudal parts of CSN and warrant a more detailed structural study of the transmission phenomenon in question.

Animals

Transfilter studies on neural induction in the newt.

In order to study the transmission mechanism of neuralising signals during primary embryonic induction, the interacting components (competent newt gastrula ectoderm and dorsal lip tissues) were separated by filter membranes of varying pore size. Nuclepore filters with nominal pore size from 0.1 to 8 mum were employed and the neuralising effect was shown to traverse all of these membranes. Electron microscopic examination did not reveal any cytoplasmic processes in the pores and the authors conclude that the morphogenetic signals are carried by transmissable compounds rather than through direct cytoplasmic contacts.

Animals

Morphogenetic interaction of presumptive neural and mesodermal cells mixed in different ratios.

Cells of the presumptive forebrain region and axial mesoderm of Triturus neurulae were disaggregated and combined in different ratios. The differentiation of the central nervous systen in these explants was dependent on the relative amount of mesodermal cells present: an increase of mesodermal cells resulted in a corresponding increase in the frequency with which caudal structures of the central nervous system developed and a gradual loss of the forebrain formations.

Animals