Determination of fludarabine in a pharmaceutical formulation by LC.
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Biomedical subjects
Publications and source records attributed to S Tommasini.
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A combination of thin-layer chromatography, high-performance liquid chromatography, UV spectroscopy and NMR spectrometry techniques for analysis of flavonoid content of Guiera senegalensis J.F.Gmel. (Combretaceae) leaves is described. Four components, catechin, myricitrin, rutin and quercetin were evidenced and determined by HPLC.
Leaf preparations of several species of Cordia are used in traditional medicine as remedies for osteoarticular diseases. The analgesic, anti-inflammatory and anti-arthritic activities in the rat of different extracts of C. francisci, C. martinicensis, C. myxa, C. serratifolia and C. ulmifolia leaves were studied. The results obtained showed that the petroleum ether and alcoholic extracts especially of C. francisci, C. myxa and C. serratifolia leaves have a significant analgesic, anti-inflammatory and anti-arthritic activity in the rat. The flavonoids and phenolic derivative content of the five species of genus Cordia leaves was investigated by TLC and determined by reversed-phase HPLC with an acetonitrile/water/acetic acid buffer solvent gradient. UV detection was carried out at 255 and 280 nm. Four flavonoid glycosides, robinin, rutin, datiscoside and hesperidin, one flavonoid aglycone, dihydrorobinetin, two phenolic derivatives, chlorogenic and caffeic acid, were evidenced and determined.
We have studied the early stages of the development of the female germ cells in the Conchostraca Eoleptestheria ticinensis Balsamo-Crivelli, 1859. The gametes originate in a scattered way throughout the tubular units of the gonad, with no development gradient recognizable. The female germ cells arise from successive karyokineses not followed by cytokinesis, within an unorganised area in which a sort of plasmodium is formed. Each primordial nucleus of this germarium develops and then forms an individual plasmic membrane. Subsequently, the usual organules differentiate in the cytoplasm. The presence of synaptinemal complexes and the beginning of the endogenous vitellogenesis by the rough endoplasmic reticulum and the Golgi apparatus qualify the previtellogenesis. The general characteristics of this early development phase of the gametes, as well as several substantial differences in the gametogenesis with respect to the other Phyllopoda studied, lead us to suggest the systematic positioning of the Leptestheriidae.
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