Biomedical subjects

S Tucci Júnior

Publications and source records attributed to S Tucci Júnior.

3 recordsLinked to original sources

PubMed1997

An experimental model for the measurement of renal function after temporary ureteral obstruction in the rat.

We present the results obtained with a ureterovesical implant after ipsilateral ureteral obstruction in the rat, suitable for the study of renal function after deobstruction in these animals. Thirty-seven male Wistar rats weighing 260 to 300 g were submitted to distal right ureteral ligation and divided into 3 groups, A (N = 13, 1 week of obstruction), B (N = 14, 2 weeks of obstruction) and C (N = 10, 3 weeks of obstruction). The animals were then submitted to ureterovesical implantation on the right side and nephrectomy on the left side. During the 4-week follow-up period serum levels of urea and creatinine were measured on the 2nd, 7th, 14th, 21st and 28th day and compared with preoperative levels. The ureterovesical implantation included a psoas hitch procedure and the ureter was pulled into the bladder using a transvesical suture. During the first week of the postoperative period 8 animals died, 4/13 in group A (1 week of obstruction) and 4/14 in group B (2 weeks of obstruction). When compared to preoperative serum levels, urea and creatinine showed a significant increase (P < 0.05) on the 2nd postoperative day in groups A and B, with a gradual return to lower levels. However, the values in group B animals were higher than those in group A at the end of the follow-up. In group C, 2/10 animals (after 3 weeks of obstruction) were sacrificed at the time of ureterovesical implantation due to infection of the obstructed kidneys. The remaining animals in this group were operated upon but all of them died during the first week of follow-up due to renal failure. This technique of ureterovesical implantation in the rat provides effective drainage of the upper urinary tract, permitting the development of an experimental model for the study of long-term renal function after a period of ureteral obstruction.

Animals↗

PubMed1994

Ploidy status correlates with outcome in stage B prostate adenocarcinoma.

1. While there are extensive data showing that aneuploidy is associated with adverse outcome in stage D prostate cancer, the utility of ploidy analysis in stage B disease is unclear. We determined ploidy in radical prostatectomy specimens from 28 patients with clinical stage B prostate cancer, and with a mean follow-up of 4.1 years (2-10 years). Patients who had no recurrences had a minimum 5 years of follow-up. Patients who had only 2 years of follow-up were included if they had developed bone metastases during this period. 2. Ploidy determinations were done on Feulgen-stained 5-microns paraffin-embedded sections using a CAS 200 image analyzer. At least 400 tumor cells were counted in every case. Tumors with at least 70% diploid cells were classified as diploid, while those with less than 70% diploid cells were classified as aneuploid. The mean percentage of diploid cells in tumors classified as diploid was 90.6 +/- 7.4, while the mean percentage of diploid cells in tumors classified as aneuploid was 36 +/- 21.9. 3. Ploidy status correlated with disease progression: seven of the 10 patients (70%) with disease recurrence had aneuploid tumors, while 13 of 18 patients (72%) who remained disease-free had diploid tumors (P = 0.03, Chi-square test). 4. These data show that patients with stage B disease with aneuploid tumors at the time of prostatectomy are more likely to have recurrent disease within a mean of 3 years (2-6 years) compared to patients with diploid tumors.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenocarcinoma↗

PubMedDate not supplied

[Placenta: a barrier for renotrophic factor?].

BACKGROUND: To evaluate if the renotrophic factor can pass through the placenta and act on the fetal kidney. MATERIAL AND METHODS: Twenty pregnant rats were divided into 4 groups of 5 animals: 1-control; 2-sham operated animals; 3-right nephfrectomy; 4-right nephrectomy--left polar nephrectomy. All rats were operated on during the 14 or 15 day of pregnancy. Maternal weight, serum urea and creatinine, number of fetal rats, fetal body and renal weight; DNA, RNA, protein and the amount of water in fetal kidneys were evaluated. RESULTS: Fetal kidneys showed a fall in weight in groups 3 and 4. In these groups tissue water concentration was lesser than groups 1 and 2. Fetal kidneys amounts of DNA, RNA and protein were similar in all groups. CONCLUSIONS: This study suggest that renotrophin could be a multifactorial system that can cross the placenta. The fall in renal fetal weight following maternal nephrectomy was consequent to a lesser tissue water content. Hypoplasia or hypotrophy did not play a role in this event. However, the fall in fetal kidney water was independent of the amount of maternal renal tissue that was removed.

Animals↗