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Biomedical subjects

S Uno

Publications and source records attributed to S Uno.

At least 73 records · Page 4Linked to original sources
PubMed1994

Glucocorticoid induction of rat angiotensin II type 1A receptor gene promoter.

Promoter/luciferase reporter analysis indicated that the 5'-flanking region of rat angiotensin II type 1A receptor gene was functional, both in the rat aortic smooth muscle cells (RASMCs) and in Y1 cells derived from mouse adrenal cortex. In response to dexamethasone (Dex), transcriptional activity of promoter increased in transfected RASMCs, suggesting the functional cis-action of a glucocorticoid responsive elements (GRE). Furthermore, the expression of the rat AT1A gene was also up-regulated by Dex at the levels of both mRNA and protein in RASMCs.

Adrenal Cortex Neoplasms↗
PubMed1994

Contraction stress and marginal adaptation of composite restorations in dentinal cavity.

The influence of contraction stress in composite resins on both the shear bond strength to dentin (BS) and marginal adaptation in a standardized dentin cavity was investigated using three restorative resin systems. A teflon ring mold (TF) or copper ring with a roughened inner surface (CP) placed on human tooth dentin was filled with the composite resin, and a shearing test was then performed. ANOVA of BS demonstrated a significant influence from the resin systems and type of molds. BSCP tended to be lower than BSTF in all three systems. This suggested that the inner surface of the CP restricted the flow of resin and then contraction stress occurred at the interface of the bond to the dentin. The system which demonstrated the smallest difference between BSTF and BSCP also showed the best adaptation. This result indicates that contraction stress in the composite resin plays an important role in marginal adaptation.

Analysis of Variance↗
PubMed1994

A possible mechanism of action of a new potassium channel opener, AL0671, on lipid metabolism in obese Zucker rats.

Antihypertensive drugs are expected to have a lipid-lowering effect for use in treating ischemic heart disease. We evaluated the effect of (+)-N-(6-amino-3-pyridil)-N'-[(1S,2R,4R)-bicyclo-[2.2.1]hept-2-yl] -N"- cyanoguanidine hydrochloride (AL0671), a newly synthesized cyanoguanidine-derivative potassium channel opener, on serum lipid and lipoprotein levels in obese Zucker rats, a genetically engineered model of type IV hyperlipidemia. AL0671 dose-dependently decreased systolic blood pressure in obese Zucker rats. Serial administration (for 1 or 2 weeks) of AL0671 (5 mg/kg/day) significantly decreased serum total triglyceride, chylomicron and very-low-density lipoprotein levels with increasing high-density lipoprotein cholesterol, whereas low-density lipoprotein levels did not change. AL0671 (5 mg/kg/day) increased lipoprotein lipase activities 4-fold and hepatic triglyceride lipase activities 3-fold in postheparin plasma. Another urea-derivative compound, AL0674, whose potassium channel-opening activity is diminished, did not affect serum lipid and lipoprotein levels. These results suggested that AL0671 activates both lipoprotein lipase and hepatic triglyceride lipase activities through its potassium channel-opening activity followed by decreasing triglyceride-rich lipoproteins in genetically obese hyperlipemic rats. Therefore, AL0671 might be beneficial in the treatment of hypertensive patients with hypertriglyceridemia (probably with insulin resistance).

Animals↗
PubMed1993

Solubility parameters, fractional polarities, and bond strengths of some intermediary resins used in dentin bonding.

An effective bonding of resin composites to dentin is generally preceded by a conditioning of the surface of the dentin. Previous studies have indicated that the intermediary or adhesive resin should have specific wetting characteristics matching those of the conditioned dentin, in order that optimum bonding can be ensured. The wetting characteristics may be expressed in terms of solubility parameter (delta) and polarity (p) of the resin. The aims of the present study were to determine these variables for a number of compounds used in adhesive resins and to investigate the effects of delta and p on the shear bond strength to dentin. Solubility parameters were obtained according to the method of Small. Fractional polarities were calculated on the basis of measurements of refractive index and dielectric constant of the resins. In the measurements of bond strength, Scotchprep, EDTA + Gluma, or Al2Ox3/glycine were used as dentin conditioners in combination with intermediary resins having various delta and p. For each conditioner, the shear bond strength (BS) could be "explained" by an exponential expression of the form BS = e(a + bx), where x = (delta + cp + d)2, and where a-d are constants depending on the conditioned dentin. It may be concluded that solubility parameter and polarity of the intermediary resins are important variables in the process of bonding to dentin.

Acrylamides↗
PubMed1993

Effect of mexiletine on elimination and metabolic conversion of theophylline and its major metabolites in rats.

In an attempt to clarify the possible mechanism of the interaction between theophylline (TP) and mexiletine (ME), the elimination kinetics and in vitro metabolism of TP and its metabolites were investigated in rats. The plasma elimination of TP, 1,3-dimethyluric acid (1,3-DMU) and 1-methyluric acid (1-MU) was significantly delayed by the intravenous (i.v.) administration of ME. The oral administration of ME also decreased the elimination rate of TP to the same extent as the i.v. dosing. The in vitro metabolic experiment showed that ME significantly inhibited the metabolic conversion of TP to 1,3-DMU and, 1,3-DMU to 1-MU, and slightly inhibited the conversion of TP to 3-methylxanthine, these processes being mediated by microsomal enzymes, with no inhibition of xanthine oxidase. Our results indicated that ME could inhibit the metabolic conversion of TP and its metabolite in rat, as reported in man.

Administration, Oral↗
PubMed1993

[Tissue concentration of intravesically instilled (2"R)-4'-o-tetrahydropyranyl-adriamycin or adriamycin in superficial bladder cancer].

Twenty-one patients with superficial bladder cancer entered an analysis of single dose (2'R)-4'-O-tetrahydropyranyladriamycin (THP) or adriamycin (ADM) administration. The patients in each group that have been or not have been treated previously with anti-cancer drugs were randomized into two groups, one was given THP and the other ADM. Thirty-mg of THP or ADM dissolved in 30 ml of physiologic saline was instilled into the bladder, and retained for 1 hour. After 1 hour retention of the drugs, tumor tissues and normal mucosas were removed by punch biopsy forceps transurethrally. The tissue concentrations of THP and ADM were estimated by high performance liquid chromatography. The tissue concentrations of THP and ADM in the tumors were significantly greater (p < 0.05) than those in the normal bladder mucosas. The tissue concentration of THP in the tumors were greater than that of ADM. The tissue concentrations of THP and ADM in the tumor of patients who have been treated with anti-cancer drugs previously were less than those of patients who have not. This results demonstrated that prior therapy with anti-cancer drug may cause a resistance for intravesical instillation chemotherapy. However in patients with prior therapy, the tissue concentration of THP in the tumors were greater than that of ADM. Based on these findings, THP has been shown to be were effective as an intravesical instillating agent especially, in cases with prior chemotherapy.

Administration, Intravesical↗
PubMed1993

[Transrectal hyperthermia for the treatment of chronic prostatitis].

A total of 36 cases with chronic non-bacterial and non-chlamydial prostatitis or prostatodynia underwent 5 weekly, 1-hour sessions of transrectal microwave hyperthermia (43 degrees C) to the prostate. All patients had a long history of the condition and failed to respond to a variety of conventional treatments. The efficacy of the treatment was evaluated by the effects on subjective symptoms and/or on white blood cells in expressed prostatic secretions. Concerning the overall clinical efficacy, excellent results were obtained in 11 (30.6%), good in 8 (22.2%), fair in 8 (22.2%) and poor in 9 (25.2%). Although minor complications were noted in 5 cases (anal pain; 2, hematospermia; 1, hematuria; 1), all cases received full sessions. These results indicated the usefulness of the hyperthermia for this benign condition, which has so far responded poorly to the conventional therapy.

Adult↗
PubMed1993

[Lymphangiomyomatosis involving the kidney: a case report].

A 58-year-old female was admitted with a chief complaint of gross hematuria. A diagnosis of carcinoma of the left kidney with lymphnode metastasis was made on the basis of computerized tomography and angiography. Left nephrectomy and lymphnode dissection were performed. Histological examination revealed lymphangiomyoma, and the final clinical diagnosis was changed to lymphangiomyomatosis with pulmonary, retroperitoneal, and left renal lesions. Lymphangiomyomatosis with renal lesions is extremely rare, and differentiation from renal carcinoma is important, though difficult.

Diagnosis, Differential↗
PubMed1992

Purification and characterization of a 7Fe ferredoxin from a thermophilic hydrogen-oxidizing bacterium, Bacillus schlegelii.

A ferredoxin (Fd) was purified from a thermophilic hydrogen-oxidizing bacterium, Bacillus schlegelii. This ferredoxin was a monomer with apparent molecular weight of 13,000 and contained 7 mol Fe/mol ferredoxin. The oxidized ferredoxin showed the characteristic EPR spectrum for [3Fe-4S]1+ (1.2 spin/mol Fd). This signal disappeared upon reduction with dithionite and new signals due to [3Fe-4S]0 and [4Fe-4S]1+ (0.7 spin/mol Fd) appeared. The quantitation of EPR signals and the iron content reveal that B. schlegelii ferredoxin contains one [3Fe-4S]1+/0 and one [4Fe-4S]2+/1+ cluster. The ferredoxin has the characteristic distribution of cysteines (-Cys8-X7-Cys16-X3-Cys20-Pro-) for 7Fe ferredoxins in the N-terminus.

Amino Acid Sequence↗
PubMed1992

Selected variables in bonding to dentin.

The aim of this study was to investigate the effect of three variables on the shear bond strength between a composite resin and dentin treated with a simplified Gluma system. Thickness of material (2 or 3 mm), distance between light guide and the surface of composite resin (0, 1, 3, or 5 mm) and irradiation time (10, 20, 40, or 60 s) were selected as variables. Thickness of material and distance between light guide and composite resin affected bond strength, but there was little influence of irradiation time under the conditions of the study. It is suggested that bond strength depends on the amount of light transmitted through the composite.

Composite Resins↗
PubMed1992

Production of human antithrombin-III in a serum-free culture of CHO cells.

A simple method was developed to establish serum-independent Chinese hamster ovary (CHO) cells that grew and secreted high level of human antithrombin-III (AT-III). First, human AT-III and mouse dihydrofolate reductase (DHFR) cDNAs were transfected into DHFR-deficient CHO cells. Transfected cells were treated with increasing concentrations of methotrexate (MTX) and clones secreting high levels of AT-III (10-20 micrograms/ml/3 day) in a serum-containing medium were obtained. Serum-independent clones were derived from the serum-dependent clones by simply culturing the cells for a few weeks in a serum-free medium. In a serum-free medium the established serum-independent clones grew at normal rate and produced almost equivalent amount of AT-III to that of the serum-dependent, parent clones. In addition, AT-III from the serum-independent clones has specific activity similar to that of plasma-derived AT-III.

Animals↗
PubMed1991

[Evaluation of renal function by dynamic CT].

Dynamic CT scans of the kidney were conducted in 57 persons with varied renal function. The results of dynamic CT were used to obtain time-density curves for renal cortex, medulla and aorta. Various parameters were calculated from these time-density curves. Among them, CA ratio, the ratio of the area under the renal cortex curve to the area under the aortic curve, showed the best correlation with creatinine clearance. With these parameters, dynamic CT studies are believed to be useful in evaluating renal function.

Adult↗
PubMed1991

Isolation of cDNA for a Xenopus sperm-specific basic nuclear protein (SP4) and evidence for expression of SP4 mRNA in primary spermatocytes.

A cDNA library was prepared in lambda gt 11 from poly(A)+ mRNA isolated from a pure population of Xenopus round spermatids and screened with an antibody against SP3-5 (sperm-specific proteins) of Xenopus sperm. Positive clones were sequenced and an arginine-rich clone, designated pXSP531, was obtained. The 473-nucleotide sequence of pXSP531 contained an open reading frame of 237 nucleotides which was preceded by a 5' untranslated region of 67 nucleotides. The 3' untranslated region contained 149 nucleotides, including a consensus polyadenylation signal (AAATAAAA). Twenty nucleotides of a poly(A) tail was contained in the pXSP531. SP3-5 were separated from each other by reverse-phase chromatography and sequenced. The amino acid sequence of the peptide fragments which were obtained by digestion of SP4 with V8 protease and separated by reverse-phase chromatography was identical to the sequence of the N-terminal 43 and C-terminal 15 amino acids deduced from the nucleotide sequence of pXSP531. This result demonstrates that pXSP531 encodes SP4. Northern hybridization of RNA extracted from primary spermatocytes and round spermatids on Days 0 and 6 with SP4 cDNA probe (pXSP531) showed that SP4 mRNA is present both in primary spermatocytes and in round spermatids as is protamine mRNA in the rainbow trout. The size of the SP4 mRNA in round spermatids on Day 0 was longer by 60 nucleotides compared to that in primary spermatocytes and that in spermatids on Day 6 was shorter by 30 nucleotides compared to that on Day 0. These size differences were due to differences in the length of the poly(A) tracts because digestion of poly(A) with ribonuclease H resulted in the shortening of mRNA to the same size for three stages.

Amino Acid Sequence↗
PubMed1991

Marginal adaptation of a restorative resin polymerized at reduced rate.

The aim of this study was to investigate the effect of reduced rate of polymerization on the marginal adaptation of a composite resin inserted in dentin cavities treated with a simplified Gluma system. The effect on bonding strength to dentin and compressive strength was also investigated. The light intensity of the polymerization unit was lowered by the use of a transformer, and thus the rate of polymerization of the composite resin. When the resin was irradiated for 30 s at 110 V followed by 30 s at 220 V, the marginal adaptation was significantly improved. This condition resulted in acceptable values of bonding strength to dentin and diametral compressive strength. It is suggested that the reduced rate of polymerization may allow for increased flow of the material, decreasing the contraction stress in the filling.

Aluminum Oxide↗
PubMed1991

Effect of 2-hydroxyethyl methacrylate in an Al2Ox3/glycine solution used as primer for dentin bonding.

The aim of this study was to investigate the effect of 2-hydroxyethyl methacrylate (HEMA) in an Al2Ox3/glycine solution used as primer for dentin bonding in combination with an experimental BISGMA-HEMA-based adhesive resin. With various amounts of HEMA in the Al2Ox3/glycine solution, a shear bond test showed a maximum strength at 50 wt% of HEMA. In this case, scanning electron microscopy examination showed a HEMA-infiltrated surface layer of the dentin along the interface between dentin and the adhesive resin. It is suggested that HEMA in Al2Ox3/glycine solutions may facilitate penetration of HEMA into etched or primed dentin.

Aldehydes↗
PubMed1991

Effect on bonding of curing through dentin.

The aim of this study was to investigate the effect of the thickness of dentin (1, 2, or 3 mm) placed between the light guide and the composite resin on the shear bond strength to dentin treated with a simplified Gluma system. The effects of the thickness of composite resin (2 or 3 mm), irradiation time (20, 40, or 60 sec), and shade (universal or brown) were also examined. The results showed that the thickness of dentin influenced bonding and interacted with the other three variables. It is suggested that dentin located between the light guide and composite resin may attenuate the light aimed at the bonding interface in the same manner as a layer of composite resin.

Color↗