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S V Kozlov

Publications and source records attributed to S V Kozlov.

36 records · Page 2Linked to original sources
PubMed1997

[A method for an areflux gastroduodenal anastomosis in the surgery of peptic ulcer of the duodenal bulb].

The authors describe a new method of areflux gastroduodenal anastomosis used for prevention of reflux gastritis after a distal resection of the stomach for ulcer disease of the duodenum bulb. It can be achieved by forming a functionally active sphincter and a "value-cusp" from the mucous-submucous layers of the frontal semicircumference of the stump of the stomach and duodenum. The method described was used in 48 resections of the stomach. Complex follow-up of the patients within the terms from 2 months till 3 years after the operations has shown a reliable areflux function of the gastroduodenal anastomosis.

Adult↗
PubMed1995

The human TAX1 gene encoding the axon-associated cell adhesion molecule TAG-1/axonin-1: genomic structure and basic promoter.

The human TAX-1 gene (HGMW-approved symbol TAX1) is located on chromosome 1 (1q32.1) and encodes the neuronal cell adhesion molecule TAG-1/axonin-1. The gene product, termed TAG-1 in the rat and axonin-1 in the chicken, is composed of six immunoglobulin (Ig)-like and four fibronectin type III (FNIII)-like domains. It is found predominantly on the axons of particular nerve fiber tracts during neural development, and it has been demonstrated to function as a potent substratum for neurite outgrowth in vitro. Here we report the cloning and structural characterization of the TAX-1 gene. The transcribed region of the TAX-1 gene extends over about 40 kb. Like its chicken homologue, the human TAX-1 gene consists of 23 exons. Two GT/CA microsatellites were localized in the first intron; a polymorphism was found for one of them. Reporter gene analysis with serially truncated fragments of the 5'-flanking region indicated that a 164-bp fragment located immediately upstream of the putative transcription initiation site was sufficient to function as a basal promoter.

Animals↗
PubMed1995

Cloning and expression analysis of the murine lymphotoxin beta gene.

Tumor necrosis factor alpha (TNF-alpha) and soluble lymphotoxin (LT) (also called LT-alpha or TNF-beta) are cytokines with similar biological activities that are encoded by related and closely linked genes. TNF-alpha, a mediator of the inflammatory response, exists in soluble and transmembrane forms. LT-alpha can be secreted or retained at the cell surface by binding to a 33-kDa transmembrane subunit, LT-beta. The recently cloned human LT-beta gene encodes another TNF family member and is linked to the TNF/LT locus within the major histocompatibility complex locus. The cell surface LT is a heterotrimer consisting of LT-alpha and LT-beta, whose physiological function is not yet clearly defined. We now report the sequence analysis of the genomic region and cDNA of murine LT-beta gene, which is closely associated with the TNF-alpha and LT-alpha genes within the murine major histocompatibility complex locus. Unlike the TNF-alpha, LT-alpha, and human LT-beta genes, which contain four exons, the murine LT-beta contains three exons and encodes a 244-amino acid polypeptide with a 66-amino acid insert that is absent from the human homologue. In situ hybridization demonstrates constitutive expression of LT-beta in lymphoid and hematopoietic tissues. LT-beta transcription is maximal in the thymic medulla and in splenic white pulp. LT-beta mRNA is also detected in the skin and in specific regions of the brain. The LT-beta promoter region contains putative Ets-binding sites, suggesting that the expression of LT-beta may be regulated in part by Ets transcription factors whose pattern of lymphoid expression overlaps that of LT-beta.

Amino Acid Sequence↗
PubMed1992

[Effect of HMG proteins and their phosphorylated forms on the process of transcription in neuronal nuclei of the rat brain (data from an immunochemical analysis)].

It was found that chromatin transcription in the neuronal nucleus of rat brain is inhibited by antisera to proteins HMG 14, HMG 17 and HMG 2. It is known that chromatin transcription of the neuronal nucleus and phosphorylation of chromosomal proteins are activated by etimizol. The phosphorylation of chromosomal proteins activated by etimizol is inhibited by the antiserum to HMG 14 (but not to HMG 17). The data obtained are suggestive of a possible role of HMG proteins and their phosphorylated forms in the regulation of transcription.

Animals↗
PubMed1985

[Alkaline phosphatase and 5'-nucleotidase in early osteogenesis in man].

By means of histochemical techniques at light and electron microscopic levels, as well as immunomorphological, biochemical and immunochemical methods localization and dynamics of alkaline phosphatase and 5'-nucleotidase contents have been determined in anlages of long tubular bones in 85 human embryos and prefetuses from the 6th up to 12th week of the intrauterine development, obtained as a result of artificial abortions in healthy women. The greatest activity of the enzymes studied is revealed in areas of an intensive osteogenesis and mineralization. Also, by means of the immunofluorescent method alkaline phosphatase of a placental type is revealed, that is not revealed, however, immunochemically. With increasing time of the intrauterine development, thermostability of alkaline phosphatase increases.

5'-Nucleotidase↗
PubMed1985

[Transcutaneous electrostimulation--method leading to a permeation system of electroanalgesia in oncological practice].

Analgetic transcutaneous electrostimulation with the newly-designed Soviet-made ELIMAN-101 installation was used for treatment of tumor-induced pain in 84 cases (as a component of general intraoperative anesthesia--in 29, for postoperative pain--54, for analgesia in cases of advanced tumor--in 11 patients). Intraoperative application of the said procedure resulted in a 2.4-fold decrease in phentanyl consumption. In early postoperative period, pain was relieved in 65.6% and alleviated in 34.5% of patients, the latter requiring non-narcotic drugs as an adjunct to electroanalgetic treatment. Transcutaneous electrostimulation offered an adequate means for management of chronic cancer pain. The procedure did not involve resistance development and ensured a nearly 6-fold decrease in opiates consumption.

Adult↗
PubMed1981

[Clinical picture and diagnosis of the Mills syndrome].

Three clinical observations of patients with Mills' syndrome are presented. The clinical peculiarity of the syndrome and the specificity of the pathological process localization in the brain revealed on pneumoencephalographic examination are stressed. The atrophic process in the fronto-parietal parasaggital divisions of the cerebral cortex is regarded as a morphological substrate of the syndrome. A conclusion on the vagueness of the process character and prematurity of the pathogenetic correlations is drawn. Nosological independence of the pathology is not improbable.

Adult↗
PubMedDate not supplied

[Effects of nitriles and amides on the growth and the nitrile hydratase activity of the Rhodococcus sp. strain gt1].

Effects of some nitriles and amides, as well as glucose and ammonium, on the growth and the nitrile hydratase (EC 4.2.1.84) activity of the Rhodococcus sp. strain gt1 isolated from soil were studied. The activity of nitrile hydratase mainly depended on carbon and nitrogen supply to cells. The activity of nitrile hydratase was high in the presence of glucose and ammonium at medium concentrations and decreased at concentrations of glucose more than 0.3%. Saturated unsubstituted aliphatic nitriles and amides were found to be a good source of nitrogen and carbon. However, the presence of nitriles and amides in the medium was not absolutely necessary for the expression of the activity of nitrile hydratase isolated from the Rhodococcus sp. strain gt1.

Amides↗
PubMedDate not supplied

[Serotonin metabolism in patients with brain tumors].

In patients with brain tumours there was revealed an increase in the content of serotonin, 5-oxyindol acetic acid (5-OAA) and melatonin in the liquor, and of serotonin in the plasma rich with platelets. The content of serotonin or products of its metabolism in the liquor, as well as of serotonin in platelets depends on the involvement of truncus cerebri and hypothalamus in the pathologic process and on the gravity of the patient's condition. The serotonin content, in contrast to 5-OAA, s significantly higher in patients with tumours attended by epileptic attacks. The data obtained testify to a possible role of serotonin in the pathogenetic mechanisms of tumours development in the central nervous system.

Adult↗