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Biomedical subjects

S V Kulikov

Publications and source records attributed to S V Kulikov.

18 recordsLinked to original sources
PubMed2006

Reversibility of changes in hepatic vessels after correction of experimental aortic coarctation.

Structural changes in hepatic vessels were studied in pups with experimental aortic coarctation and animals with corrected defect. Reconstruction of vessels was assayed by functional, morphometric, and histological methods. Aortic coarctation in pups was followed by a decrease in circumferential strain of the wall of hepatic arteries. These changes were accompanied by atrophy and sclerosis of the media. The arterial bed had a greater number of vessels with intimal muscles. Muscle bundles in large hepatic veins underwent dystrophy. After correction of experimental defect, the increase in circumferential strain of arteries was accompanied by hypertrophy of the wall. The number of arterial vessels with intimal muscles decreased, while muscle bundles in hepatic veins were thickened. Sclerosis of hepatic vessels was reversible.

Animals↗
PubMed2003

[Morphological changes of hepatic vessels in experimental aortal coarctation and after its elimination].

Structural peculiarities of hepatic vascular bed were studied in 10 intact dogs, 15 pups with hemodynamic model of aortal coarctation (AC) and in 10 animals after AC elimination. To detect the regularities of remodeling of arteries and veins of this organ, morphometric and histological methods were used. In the arteries, branches of portal and hepatic veins AC caused the reduction of the tone and attenuation of the media, associated with the decrease in number and dimensions of smooth muscle cells. Simultaneously, as a result of adaptation, in the intima of the vessels carrying blood to the liver (arteries) the degree of the development of regulatory muscular structures was increased, while similar structures in the blood outflow vessels (hepatic veins) were reduced. Along with adaptational modifications, some pathological changes took place in the liver, which were manifested by sclerosis of vascular walls and stroma. After AC elimination and restoration of hemodynamics, the tone of the vessels was found to increase with the hypertrophy and hyperplasia of the smooth muscles in their media. The degree of development and the number of regulatory structures in the liver inflow vascular bed were reduced, while those in the outflow bed were increased. At the same time, the process of reversal of sclerotic changes in hepatic blood vessels was initiated.

Animals↗
PubMed2001

[Structural changes in the intraorgan hepatic arteries during experimental aortic coarctation].

Structural changes of intraorganic hepatic arteries were studied in 8 control dogs and 20 pups with hemodynamic model of aorta coarctation. Experimental animals were observed within the terms from 1 month to one year. Histological and morphological methods were used to assess the state of hepatic vessels. The investigations performed resulted in the discovery of the complex of adaptive and pathological changes in the hepatic arterial bed. The first were the reactive atrophy of the hepatic arteries wall with decrease of smooth myocytes number and parameters in the media and the appearance of musculo-elastic sphincters, Conti pillows and smooth myocytes bundles in the intima. The latter lied in the arterial and arteriolar wall sclerosis in liver. Pathological changes grow proportionally with the terms of the experiment.

Animals↗
PubMed1995

[A granulopoiesis inhibitor. Synthesis and biological activity].

Three schemes of synthesis for pentapeptide Glp-Glu-Asp-Cys-Lys-OH were compared was carried out. Acetamidomethyl protection was used for the mercapto group of cysteine. For the same purpose, cystine was used as the starting compound for synthesis. The optimal method was shown to be the solid phase method with S-acetamidomethyl cysteine protection that can be removed by mercuric acetate before the cleavage of a peptide from a polymer. The stabilized peptide inhibits proliferation of bone marrow cells of patients with chronic myeloleukemia 5- to 20-fold and has a less pronounced effect (up to 2-fold inhibition) on peripheral blood cells. Thus, its application for the therapy of hemoblastoses is promising.

Amino Acid Sequence↗
PubMed1995

[The effect of certain factors on binding of tert-butyloxycarbonylamino acids to Merrifield polymer during interphase catalysis and assessment of racemization of the bound amino acid].

In order to reduce the influence of hydrogen bonds on the acylamino acid salts attachment to the chloromethylated resin, it is proposed to use compounds that can compete for the hydrogen bonds formation. The best solvent proved to be hexamethylphosphoric triamide. Use of interphase catalysts, e.g., tributyl-p-nitrobenzyl ammonium, also gives good results. The racemization degree of the amino acids attached to solid support by means of the interphase catalysis does not exceed that of amino acids loaded on the polymer according to Gisin's method.

Amino Acid Sequence↗
PubMed1993

[Use of arylsulfenylchlorides for cleaving fibrinogen at methionine residues].

Conditions for the fibrinogen fragmentation with 2-pyridyl-or o-nitrophenylsulfenylchloride were proposed. Resulting mixture of fragments is identical to the mixture prepared by the cyanogen bromide treatment and is suitable as supplementary analytical reagent in the determination of the tissue plasminogen activator (TPA) activity with a chromogenic substrate.

Cyanogen Bromide↗
PubMed1982

[Repair effect of p-aminobenzoic acid and aminobenzhydrazide].

A simple, safe and rapid experimental scheme is offered for screening chemical compounds for ability to activate DNA reparation in bacterial cells. As DNA-damaging agent use was made of cell heating. The structural integrity of genome was determined during subsequent 90-minute incubation of bacteria. It was demonstrated that without additions to the culture medium, the cells are unable to recover the damaged genome, while addition of the compounds in question stimulated the reparation of bacterial DNA. p-Aminobenzoic acid (10(-5) M) was more powerful than aminobenzhydrazide as regards the recovery of genome integrity.

4-Aminobenzoic Acid↗
PubMedDate not supplied

[Effect of some imidazole-4,5-dicarboxylic acid derivatives on the activity of N-methyl-D-aspartate (NMDA) receptors].

The results of experiments on mice showed that some imidazole-4,5-dicarboxylic acid derivatives injected into lateral cerebral ventricles produce a dose-dependent convulsant or anticonvulsant effects, that is, possess the properties of partial NMDA receptor agonists. The most promising partial NMDA receptor agonist selected for further investigation is 2-propylimidazole-4,5-dicarboxylic acid.

Animals↗
PubMedDate not supplied

[The analgesic action of new enkephalin analogs].

The enkephalin analogue peptide IKB-901 containing epsilon-ACA and cysteine with the modified S-end shows an analgetic activity in rats (1 micron, intrathecally and 5 mg/kg intravenously) and in cats (0.35 and 0.7 mg/kg intravenously). Naloxone (0.1 mg/kg) prevents the analgetic effect of peptide. The coadministration of the peptide and the enkephalinase inhibitor D-phenylalanine (0.35 and 10 mg/kg, respectively) enhances analgesia and displays an antihypertensive effect in nociceptive stimulation.

Analgesics↗
PubMedDate not supplied

[Immunoenzyme method of determining trace amounts of proinsulin in recombinant human insulin].

An enzyme immunoassay (ELISA) for the detection of human proinsulin has been developed based on the interaction of the absorbed proinsulin with the antiserum against the synthetic proinsulin C-peptide. In the presence of high concentrations of insulin, the sensitivity of the assay slightly decreases due to the competitive adsorption of insulin onto the polystyrene carrier with the binding constant 800-1000 times less than that of proinsulin. A method is proposed for the interpretation of ELISA data based on analysis of a weighed dry insulin sample, which enables the detection of a 0.02-0.1% admixture of proinsulin in the chromatographically purified recombinant human insulin.

Chromatography, High Pressure Liquid↗