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Biomedical subjects

S V Lysenko

Publications and source records attributed to S V Lysenko.

11 recordsLinked to original sources
PubMed1991

[Microorganisms synthesizing enzymes with thrombolytic action].

The literary and experimental data obtained by the authors concerning the ability of various microorganisms to synthesize exoproteases lysing the thrombi of human blood are presented in the article. Various methods of enhancing the thrombolytic activity of microorganisms cultures, the properties of some isolated and purified preparations, methods of enlarging the specificity of the enzymes to fibrin and of reduction of their toxicity are considered.

Bacteria↗
PubMed1978

Upper boundary of the biosphere.

By using meterological rockets fitted with specially designed analyzers, samples for microbiological investigation have been taken. The analyzer design prevented extraneous microorganisms from penetrating into the analyzer. Before being used, the analyzers were sterilized with high gamma-ray doses. For the first time microorganisms have been detected in the mesosphere at an altitude of 48 to 77 km. The microorganisms are microscopic fungi having black conidia or spores (Circinella muscae, Aspergillus niger, Papulaspora anomala) and one species forming green conidia (Penicillium notatum). Colonies of Mycobacterium luteum and Micrococcus albus have also grown. Five of the six species have synthesized pigments. The presence of pigmented microbial forms leads us to believe that natural selection is occurring in the mesosphere because cells possessing chromogenous pigments (carotenoids, melanins) are more resistant to ultraviolet-ray action. A greater number of microorganisms have been registered in the mesosphere during dust storms than in the absence of strong winds.

Air Microbiology↗
PubMedDate not supplied

[Specificity of action of extracellular proteases from Streptomyces spp].

The ability of an Actinomyces strain--Streptomyces spp. to produce extracellular proteases has been studied under varied cultivation conditions during the growth cycle. The activity of enzyme preparations precipitated from the culture liquid was determined with various substrates--gelatin, casein, fibrinogen, fibrin and collagen. The isolated enzyme complex possessed caseinolytic, fibrinolytic, thrombolytic and collagenolytic activities.

Caseins↗
PubMedDate not supplied

[Fibrinolytic activity of bacteria from Pseudomonas genus].

A strain of the genera Pseudomonas genera was found to possess hemolytic, fibrinolytic and thrombolytic activities. The fibrinolytic activity of the lyophilized unpurified preparation was 900 conventional units/mg. After incubation in the blood plasma, the activity completely remained. The preparation (1 microgram/ml, 750 micrograms of protein) obtained by precipitation with ammonium sulfate (80% saturation) completely lysed in vitro human blood thrombi for 50 min. The strain studied can find practical applications in medical industry.

Culture Media↗
PubMedDate not supplied

[Paramagnetic properties of the conidial pigments of Aspergillus niger and Penicillium notatum fungi isolated from the mesosphere].

The conidia of Aspergillus niger and Penicillium notatum detected in the upper atmospheric layers of the Earth (58--77 km) were found to contain stable paramagnetic centres (PMC) at concentrations of 0.2 X 10(18) and 1.6 X 10(18) per gram of dry biomass, respectively. Aspergillin, the black pigment of Asp. niger, was shown to have (0.1--0.6) X 10(18) PMC per gram of dry substance (depending on the fraction). Stable paramagnetism of the conidial pigments with respect to their active protecting action in vivo is discussed on the basis of these data.

Aspergillus niger↗
PubMedDate not supplied

[Microorganisms of the upper layers of the atmosphere].

Samples for microbiological studies were taken at a high altitude using meterological rockets with specially constructed analyzers so that no extraneous microorganisms could get into them before sampling. The analyzers were sterilized by gamma-irradiation before sampling. For the first time, microscopic fungi, bacteria and mycobacteria were detected at a height of 48--77 km. Five among six of the microbial species were found to contain pigments. The prevalence of these forms at high altitudes suggests that natural selection may take place in the upper atmospheric layer since cells containing chromogenic pigments (carotenoids, melanins) are more resistant to the action of ultraviolet rays.

Air Microbiology↗
PubMedDate not supplied

[The protective role of pigments against UV rays in fungi isolated from the mesosphere].

Five among six species of microorganisms isolated from the mesosphere contained pigments which made them more resistant to the action of UV as compared to pigmentless microorganisms in the atmosphere of Earth. UV irradiation in the atmosphere is supposed to select resistant pigmented forms, so that they predominate in the mesosphere. To confirm this assumption, mutants of Aspergillus niger, Penicillium notatum and Circinella muscae were sported by irradiating them four times and then subjecting to stepwise selection. These mutants either synthesized pigments at a very low rate or did not produce them at all. No significant differences were found by studying the biomass, mycelium and sporeforming organs of the parent cultures and their mutants. However, their resistance to UV was not the same. Addition of the pigment apsergillin, isolated from the conidia of Aspergillus niger, to a suspension of the pigmentless (mutant) conidia of Penicillium notatum, the spores of Circinella muscae, and the vegetative cells of Micrococcus albus, before their irradiation with UV, considerably increased their resistance to this factor.

Atmosphere↗
PubMedDate not supplied

[Optimal conditions for determing ATP of microbial origin].

Endogenous luminescence of the luciferin-luciferase extract from firely tails was studied in determing low concentrations of ATP. The optimum concentration of the the extract, corresponding to the minumum effect of endogenous luminescence, was found in the reaction medium. The solution of luciferin-luciferase was unstable at room temperature and upon dilution. The activity of luciferin-luciferse solutions did not change after freezing in liquid nitrogen with following thawing. The content of ATP was assayed in the cells of Sarcina flava disintegrated with ultrasound.

Adenosine Triphosphate↗