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Biomedical subjects

S V Shabalina

Publications and source records attributed to S V Shabalina.

At least 19 recordsLinked to original sources
PubMed1988

[Indices of diphtheria (antitoxic) immunity in the dynamics of the disease and its treatment in adults].

A total of 1,034 serum samples from 618 persons, including patients with different forms of diphtheria, carriers of the toxigenic forms of Corynebacterium diphtheriae, and angina patients, were studied. Analysis of the incidence of antibodies to C. diphtheriae toxin and their titers revealed that in more than half of all diphtheria patients no antibodies to C. diphtheriae toxin were detected upon admission to hospital. At the same time in 26% of the patients no antibodies were detected during the whole period of the disease; in such patients the toxic and subtoxic forms of diphtheria were registered twice as often as in seropositive patients. In 31% of the patients seronegative by the moment of hospitalization a rapid increase in the titers of antibodies C. diphtheriae toxin was observed in the course of the disease, which was indicative of the secondary character of immune response in patients who had been immunized earlier.

Adult↗
PubMed1987

[Detection of diphtheria toxin and its degradation products by using immunoenzyme analysis].

An enzyme immunoassay system for the detection of diphtheria toxin and the products of its degradation has been developed on the basis of the enzyme-linked immunosorbent assay. To sensitize the assay plates, bivalent F(ab)2-fragments of purified antidiphtheria antibodies at a concentration of 10.0 micrograms/ml, obtained from the blood serum of hyperimmunized rabbits, have been used. Specific conjugates have been prepared with the use of F(ab)2-fragments of purified antidiphtheria antibodies obtained from the blood serum of hyperimmunized horses. The optimum time and temperature conditions of the assay have been established. The new enzyme immunoassay system permits the detection of diphtheria toxin and the products of its degradation in biological substrates at a concentration of 10.0-5.0 ng/ml.

Animals↗
PubMed1985

[Development of a diagnostic test system for detecting soluble staphylococcal antigens by an immunoenzyme method].

ELISA is used for detecting the soluble staphylococcal antigen in patients with purulent septic infections. The optimum conditions for the assay have been established: the dose of staphylococcal gamma globulin for plate sensitization should be 5.0-10.0 micrograms/ml, the pH of the buffer solution 9.6-10.0, the time and temperature of incubation 18-20 hours at 4 degrees C or 5 hours at 37 degrees C. The possibility of using plates manufactured in the USSR has been shown. The sensitivity of the above diagnostic test system is 0.005 microgram/ml.

Antigens, Bacterial↗
PubMed1983

[Diagnostic test system for the quantitative determination of Shigella antigens in patient blood by an immunoenzyme analytical method].

The diagnostic test system under trial has been shown to permit the detection of S. sonnei and S. flexneri specific antigens with an accuracy of 10(-3) micrograms. Along with high sensitivity, the test system has sufficiently high specificity. Statistically significant differences in the occurrence of specific dysentery antigens and their levels in the blood of dysentery patients and healthy persons have been revealed.

Acute Disease↗
PubMed1983

[Diagnostic test-system for the immunoenzymatic detection of meningococcal antigen].

To detect meningococcal antigen, the use of the enzyme-labeled immunosorbent assay (ELISA), a new variant of the immunoenzymatic method, permitting one to carry out quantitative analysis, is proposed. The optimum conditions for the test to detect group A meningococcal antigen, as well as the procedure for the approbation of the test on patients with meningococcal infection and on healthy persons, have been worked out. The method is shown to be highly specific and sensitive.

Animals↗
PubMed1981

[Parameters for constructing a test system for the isoenzymatic detection of meningococcal antibodies].

The optimum conditions for the detection of specific humoral antibodies in patients with meningococcal infection by the immunoenzymatic method have been determined: the sensitizing dose of the antigen is 20 microgram of protein per 1 ml for group A meningococcal antigen and 25 microgram of protein per 1 ml for group C meningococcal antigen, the pH of the buffer solutions is 5.0-6.0 for group A meningococcal antigen and 12.0 for group C meningococcal antigen, the time and temperature of incubation should be 18-20 hours at 14 degrees C. The diagnostic test system developed in this research is capable of detecting antibodies with the accuracy of several ng.

Antibodies, Bacterial↗
PubMed1979

[Immunoglobulin level in viral hepatitis patients].

The results of studying the dynamics of serum immunoglobulins in patients with viral hepatitis varying in severity are presented. At the acute stage of the disease pronounced shifts in the content of all the three classes of immunoglobulins were found to occur in the patients irrespective of their age. Higher levels of IgM detected in women seem to be due to the physiological peculiarities of the female organism. Gamma globulin prophylaxis, when carried out at the incubation period, has been shown to exert a negative influence on humoral immunity.

Adolescent↗