PubMed Health⌕ Search

Biomedical subjects

S V Shirshev

Publications and source records attributed to S V Shirshev.

At least 37 records · Page 2Linked to original sources
PubMed1991

[The significance of chorionic gonadotropin in regulating the antigen-independent differentiation of immunocompetent splenic cells].

The functional activity of fractionated splenocytes were investigated in the syngeneic transfer system. Chorionic gonadotrophin (CG) was injected to ovariectomized and noncastrated lymphocyte donors, the T or B lymphocytes being isolated on a column with a nylon fiber and by means of anti-BaO-serum accordingly. In some series CG was introduced only into recipients before their being irradiated and before syngeneic cells transferring. CG has been stated to be capable of influencing either independently or by means of ovarian hormones splenic B lymphocytes without exhibiting a significant effect on T helper precursors as well as radioresistant cells of the splenic stroma. The character of immunoregulating effect was completely dependent on the presence of ovaries in mice and on the hormone dose.

Animals↗
PubMed1987

[Effect of chorionic gonadotropin on the cooperative interrelations of splenocytes involved in the primary immune response].

The influence of chorionic gonadotropin (CG) on the interaction of spleen T- and B-cells has been studied in adoptive transfer system. It has been established that CG increases the primary immune response in non-ovariectomized mice. This effect reversely depended on the hormone concentration and was independent of prostaglandins (PG). In the experiments on ovariectomized mice the influence of CG was opposite and the immune response was decreased. This action was completely abolished by Voltren-induced blockade of UG-synthetase. In spleen cell culture of female mice CG suppresses the synthesis of interleukin-2 (IL-2). It is suggested that CG may have an independent immunosuppressive action, the mechanism of which consists of disturbed cell-to-cell communications on the level of short-acting mediators of the immunity--PG and IL-2.

Animals↗
PubMedDate not supplied

[Role of chorionic gonadotropin in the differentiation of thymocytes].

The effects of chorionic gonadotropin, a basic hormone of pregnancy, on the differentiation of the human thymocytes were studied. The hormone does not affect the phenotype as determined by expression of the membrane molecules CD3, CD4, CD8 and functional activity of intrathymic pre-T-lymphocytes, but stimulates production of autocrine growth factors by these cells. Cultivation of cortical thymocytes in the presence of chorionic gonadotropin induces their phenotypic maturation with predominant development of CD4+ CD8- cells. In addition, at a high physiological dose (100 MU/ml) the hormone induces functional maturation of the cortical thymocytes. Thus, the data obtained allow us to consider this hormone as a factor regulating antigen-independent differentiation of T-lymphocytes during pregnancy and determining development of the immune system in embryogenesis.

CD4-CD8 Ratio↗
PubMedDate not supplied

[Effect of chorionic gonadotropin on thymocyte differentiation in the presence of thymus epithelial cells].

We studied the effects of the main placental hormone, chorionic gonadotropin, on differentiation of human thymocytes in vitro in the presence of thymic epithelial cells. It was shown that the hormone at a high dose (100 IU/ml) enhanced the epithelium-induced phenotypic maturation of thymocytes, which is registered by an increased expression of the membrane marker CD3 and transition of CD4+8+ thymocytes in the cells with CD4+8- and CD4-8+ phenotypes. In addition, gonadotropin enhanced the proliferative response of thymocytes to the mitogen during their cultivation with the epithelium. The stimulating effect of the hormone on the epithelium-induced differentiation of thymocytes is mediated by the humoral factors of epithelial cells. In addition, gonadotropin at this dose exerts its own differentiating activity with respect to thymocytes and stimulates their phenotypic and functional maturation in a monoculture.

Cell Differentiation↗