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Biomedical subjects

S V Zaĭtsev

Publications and source records attributed to S V Zaĭtsev.

At least 19 recordsLinked to original sources
PubMed2003

[Effect of medications and acupuncture on indicators of a bioenergetigram of bronchial asthma patients].

AIM: To evaluate the energy informational effects of various treatments in patients with bronchial asthma (BA). MATERIAL AND METHODS: Changes in bioenergograms were analyzed in 139 patients after therapeutical exposures: intravenous prednizolone and dexamethasone, inhaled seretide, fluthicasone propionate, beclomethasone, phenoterol, salmeterol, and salbutamol, the latter drug as tablets, as well as a course treatment with acupuncture. The energy informational effects of pumpan and nitrosorbide were studied in 22 patients with BA concurrent with cor pulmonale and coronary heart disease (CHD). RESULTS: A significant difference was found in the parameters of a bioenergogram depending on the routes of administrations of the drugs and their dosage forms. The greatest and positive changes in the area of the bioenergogram were recorded when using salmeterol and salbutamol, particularly in the nebulization of their solutions, and acupuncture. The intravenous infusion of glucocorticosteroids frequently produced inhibitory effects. In patients with BA concurrent with cor pulmonale and CHD, the positive energy informational effect of pumpan, that differed from that of nitrosorbide, occurred with better ventricular repolarization and alleviated signs of right cardiac overload on ECG. CONCLUSION: The bioenergogram is highly sensitive to changes in the status of patients and it may be used to choose drugs and their combinations, combined drug therapy, and acupuncture on an individual basis. Pumpan is recommended for the treatment of patients with BA concurrent with cor pulmonale.

Acupuncture↗
PubMed1994

[Participation of opioid receptor of the kappa-, mu-, and delta-types in regulating the humoral immune response].

The alteration of opioid receptor total binding level as well as ae- and mu-, delta-type distribution has been investigated on the every day of primary and secondary immune response on bovine gamma-globulin. It has been shown that a level of opioid binding measured with [3H]naloxone increased by 6 times during the primary, and by 1.5 times during the secondary immune response on the antigen. A maximal value of opioid receptor activity has been observed at the 7-th and the 5-th days correspondingly, i.e. at the peak of the response. Receptor activity has been measured by displacement of [3H]naloxone with corresponding selective opioid ligand. Growth of the quantity of ae-receptors and decrease of that of mu- and delta-receptor types has been found to take place starting with zero time till 7-th and 5-th days of response development. A reverse picture has been detected during the immune response falling down. Selective ae-ligand stimulated the secretion of specific antibodies to bovine gamma-globulin, whereas mu- and delta-ligands suppressed the antibody production. Functional role of different types of opioid peptides taking part in immune regulation is analyzed.

Animals↗
PubMed1994

[Synthesis and biological activity of new analogs of beta-casomorphine-5].

Four new analogues of beta-cazomorphine-5 modified at the C-end with ethylenediamine- and glycine-containing derivatives were synthesized by the standard method of peptide chemistry (mixed anhydrides, carbodiimide, activated esters): H-Tyr-Pro-Phe-Pro-Gly-EtDA-Gly-II (I) H-Tyr-Pro-Phe-Pro-Gly-EtDA-Gly-Gly-II (II) H-Tyr-Pro-Phe-Pro-Gly-EtDA-Gly-Gly-CO-CH3 (III) H-Tyr-Pro-Phe-Pro-Gly-EtDA-Gly-Gly-CO-CH2-CH2-COOH (IV) The level of affinity and the degree of selectivity of the peptides towards the mu- and delta-opioid receptors of the rat brain lyophilized membranes were studied by the radioreceptor method. All the new peptides displayed analgetic activity, largely depending upon their structure.

Amino Acid Sequence↗
PubMed1993

[The role of membrane lipids in regulating the function of neuromediator receptors].

The role of membrane lipids in the interactions between neurotransmitters and membrane-bound receptors has been studied with regard to: (i) the specificity of interactions between the lipids and the neurotransmitter molecules; (ii) the modulation of the receptor binding activity by annular lipids and (iii) the physical state of the membrane lipid bilayer as a whole. These aspects are discussed for various receptor systems. Particular emphasis has been placed on the lipid regulation of the ligand binding to opioid receptors. A new methodological approach to the study of receptor regulation by lipids is considered. This approach consists in the study of spontaneous inactivation of selective ligands binding as applied to the study of lipid regulation of opioid receptors in rat brain.

Animals↗
PubMed1993

[Inactivation of naloxone binding with opioid receptors in rat brain membranes].

Inactivation of naloxone binding to rat brain membrane-bound mu-opioid receptors (37 degrees C) was studied using the radioligand method. The rate inactivation constant (k(in) = 5.2 +/- 1.0 x 10(-3) min-1), the activation energy (Ea = 82.5 kJ/mol) and the change in the activation entropy (delta S = -14 kJ/K.mol) were determined. Incubation with unsaturated fatty acids (linoleic and arachidonic acids, 10(-7)-10(-4) M), cardiolipin (10(-5)-10(-4) M), phosphatidylinositol (10(-5)-10(-4) M), 2.6-ditretbutyl-4-exitoluene (10(-4) M), and argon produced a significant stabilizing effect on naloxone binding. The microviscosity changes in the ESR probe (2,2,6,6-tetramethyl-4-capryloyloxypiperidine-1-oxyl) environment and peroxidase oxidation of membrane lipids during incubation of rat brain membranes were investigated. It was suggested that the main reason for the decrease in the opioid receptor activity is the peroxidation of unsaturated acid lipids in the specific receptor environment and the reduction of the lipid viscosity.

Animals↗
PubMed1992

[A comparative study of met-enkephalin effects on the secretion by murine lymphocytes of antibodies to different antigens].

The influence of met-enkephalin on specific antibody production by lymphocytes from mouse lymph nodes was studied in vitro in productive phase of immune response. It was shown that the peptide did not influence secretion of IgM-antibody to T-independent antigen-trinitrobenzensulfoacidic group, but suppressed secretion of IgG-antibody to T-dependent antigens both during primary and secondary response. The efficiency of superlow concentrations of the peptide (10(-15)-10(-14) M) for the response to ovalbumin, but not for the response to bovine gamma-globulin was shown. All effects of met-enkephalin were naloxone-reversible. The existence of individual distribution in dose-dependences of peptide action on antibody secretion in response to ovalbumin was demonstrated.

Animals↗
PubMed1992

[Heterogeneity of mu- and delta-opioid active sites in the mouse cerebral cortex. Selective increase in the affinity of a superhigh affinity delta-site during long-term administration of morphine].

The interaction of the selective mu-ligand [D-Ala2, MePhe4, Glyol5]enkephalin (DAMGO) and the selective delta-ligand [D-Ser2, Leu5, Thr6]enkephalin (DSLET) with the binding sites in rat brain cortex membranes, has been studied. Analysis of the binding isotherms in the presence and absence of nonlabelled DAMGO and DSLET revealed that these ligands can interact with both superhigh affinity and high affinity binding sites that are distinct from one another. Prolonged administration of morphine and formation of physical dependence caused no appreciable changes in the parameters of either the superhigh affinity binding sites for the mu-ligands or the high affinity binding sites for mu- and delta-ligands. At the same time, the affinity of the superhigh affinity site for delta-ligands increased 3.5 times (tau 1/2 approximately equal to 17.5 h).

Animals↗
PubMed1992

[Interaction of (3H)naloxone with immunocompetent murine cells: the effect of mitogenic and antigenic activation].

The binding of the opioid antagonist [3H]-naloxone to immunocompetent cells of the mouse, F1(CBA x C57B1/6), in medium 199 has been studied. The binding was reversible and reached a maximum during 15-20 min at 37 degrees C. The stereospecificity profile was proven to correspond to mu-type receptors. The binding curve was characterized by high positive cooperativity (nH = 2.3, IC50 = 5 nM). Mitogenic stimulation by Con A, SEA, and ML caused an increase in the number of receptors. Besides, stimulation by an antigen (ovalbumin) changed the binding parameters. The distribution of binding sites for naloxone on various immunocompetent cells was investigated. The maximal number of sites was found on lymphocytes of lymph nodes and bone marrow cells. A conclusion is drawn that both T- and non-T-cells play a role in naloxone binding.

Animals↗
PubMed1992

[Effect of superlow doses (10(-18)-10-(-14) M) of biologically active substances: general rules, features, and possible mechanisms].

The effects of ultra-low (10(-18)-10(-14) M) doses (ULD) of biologically active substances have been reviewed in terms of common regularities of ULD effects and peculiarities of action of various groups of compounds. The most common and at the same time paradoxical regularities of ULD action are bi- or polymodal patterns of dose dependence, absence or presence of an inverse effect at higher doses, and instability of ULD effect. Possible mechanisms of ULD action including the mechanism based on the adaptation theory are discussed.

Adaptation, Physiological↗
PubMed1991

[Binding of FITC-labelled alpha-thrombin by peritoneal mast cells].

The interaction of bovine alpha-thrombin with peritoneal mast cells was studied using FITC-labeled enzyme. Thrombin was modified with FITC in the presence of heparin and was separated from heparin and free FITC by gel-filtration at HPLC yielding FITC-labeled alpha-thrombin with intact additional recognition binding site for high molecular substrates and cell receptors. Equilibrium studies have shown that the binding of thrombin to peritoneal mast cells is active independent, rapid, specific, saturable and reversible. Equilibrium between bound and free thrombin is attained within I min and Scatchard analysis indicates a population of approximately 54 x 10(3) sites/cell with a dissociation constant of 1.3 x 10(-9) M. FITC-labeled alpha-thrombin binds to peritoneal mast cells in a temperature-dependent manner with optimum at 37 degrees C. These results indicate that FITC-labeled alpha-thrombin binds to peritoneal mast cells with high affinity.

Animals↗
PubMed1990

[Kinetic characteristics of neuroleptic binding sites on human lymphocytes].

The kinetics and equilibrium of the interaction of [3H]spiperone with binding sites on human lymphocytes have been studied using radioligand analyses. The process of [3H]spiperone binding to these sites can be explained by the model of ligand interaction with two independent binding sites (high-affinity site, KD1 = 3 nM, and low-affinity site, KD2 = 20 nM), thus confirming the heterogeneity of the sites on human lymphocytes.

Antipsychotic Agents↗
PubMed1990

[Kinetic characteristics of the effect of GTP on properties of opioid receptors].

Specific interactions of guanine nucleotides with rat brain membrane preparations were investigated; a two-step scheme of this interaction was proposed. The effect of GTP on the agonist binding to rat brain opioid receptors was studied. Nucleotides were shown to inhibit the ligand interactions with mu- and sigma-receptors. It was found that the GTP-induced inhibition differs from that of the GTP stable analog, GppNp, i.e., GTP does not initiate any noticeable dissociation of the receptor system component and its liberation from the membrane into solution as is the case with GppNp. A kinetic model of the GTP interactions with the opioid receptor system stipulating that the affinity of high affinity centers for mu- and delta-ligands changes after nucleotide hydrolysis on the N-protein, was proposed. The differences in the mechanisms of GTP effect on mu- and sigma-receptors were revealed, i.e., the inhibition of the ligand binding to mu-receptors may occur just after GTP binding to the N-protein. The ligand-receptor interactions in the presence of GTP were modelled according to the proposed scheme.

Adenine Nucleotides↗
PubMed1989

[Lyophilized membranes as a test system for analyzing the properties of neuromediator receptors and activity of neurotropic agents].

The effect of lyophilization and prolonged storage of rat brain membrane preparations on the properties of receptors of cholinergic and adrenergic neuromediator systems has been studied by the radioreceptor assay. Lyophilized membrane preparations have been shown to be highly stable as compared with fresh membranes and retain their binding properties during storage for 1 year.

Animals↗
PubMed1989

[Survival rate of Leptospira pomona in the soil at a natural leptospirosis focus].

At the area of a natural focus of leptospirosis (caused by L. pomona) in the Mozdok region of the North Occetic ASSR leptospires were detected by the method of dark field microscopy in 22% of intact soil samples. The presence of pathogenic leptospires in this soil was not confirmed by the method of the biological assay on Syrian golden hamsters. In controlled tests lasting 1-277 days, in 30.4% of cases L. pomona retained their viability, pathogenic and antigenic properties for as long as 74 days, while staying in the soil at the focus of infection with humidity being 15.2-31.4% and pH = 6.7-7.2. 11 Leptospira cultures isolated after staying in the soil retained their pathogenic properties and the death of the animals used in the bioassay from the acute form of leptospiral infection.

Animals↗