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S Vivekanandan

Publications and source records attributed to S Vivekanandan.

16 recordsLinked to original sources
PubMed2004

Apolipoprotein-D: a novel cellular marker for HGPIN and prostate cancer.

BACKGROUND: High grade prostatic intraepithelial neoplasia (HGPIN) is a putative pre-malignant lesion of the prostate. While apolipoprotein-D (Apo-D), an androgen-regulated hydrophobic transporter protein, is expressed in prostate tumors, its expression in HGPIN is unknown. METHODS: Immunoreactivity for Apo-D and another androgen-regulated protein, prostate specific antigen (PSA), was investigated in 64 radical prostatectomy tissues by video image analysis. RESULTS: Eighty two percent of prostatectomy specimens demonstrated moderate to strong Apo-D immunoreactivity in areas of HGPIN. In comparison, weak Apo-D immunoreactivity was observed in non-malignant areas in only 24% of specimens. The median (range) percentage cellular area of HGPIN immunopositive for Apo-D (9.7%, 0-42.9), and the cellular concentration of Apo-D (MIOD 3.1, 0-13.3), were intermediate between that of normal (area 0%, 0-53.5%, MIOD 0, 0-12.6) and early stage prostate cancer tissues (area 29.2%, 0-90.8%, MIOD 6.7, 0-28.1). This increase in Apo-D expression from non-malignant, through HGPIN to prostate cancer was statistically significant (P < 0.001), and contrasted with the decrease observed in PSA staining between adjacent areas of normal glands, HGPIN, and cancer (P = 0.026). CONCLUSIONS: The presence of high levels of immunoreactive Apo-D in HGPIN and prostate cancer, but not in non-malignant epithelial cells, is consistent with HGPIN being an intermediate lesion in the transition to prostate cancer, and suggests that cellular Apo-D expression is a marker of malignant transformation of the prostate.

Apolipoproteins↗
PubMed2002

Cross-reaction of human chorionic gonadotrophin in Immulite 2000 luteinizing hormone assay.

BACKGROUND AND METHOD: The cross-reactivity of human chorionic gonadotrophin (hCG) in the new DPC Immulite 2000 luteinizing hormone immunoassay was studied using sera from healthy pregnant women and pooled serum spiked with hCG standard IRP 75/537. RESULTS AND CONCLUSION: Significant positive bias was seen in sera from pregnant women and in IRP 75/537 spiked serum.

Amenorrhea↗
PubMed2000

Chloramine-B sesquihydrate.

In the title compound, sodium N-chlorobenzenesulfonamide sesquihydrate, Na(+).C(6)H(5)ClNO(2)S(-).1.5H(2)O, the sodium ion exhibits octahedral coordination by O atoms from three water molecules and by three sulfonyl O atoms of three different N-chlorobenzenesulfonamide anions. A two-dimensional polymeric layer consists of units, each comprising two face-sharing octahedra which share four corners with four other such units, the layer running parallel to the ab plane. The water molecules participate in hydrogen bonds of the types O-H...O, O-H...N and O-H...Cl.

Chloramines↗
PubMed1998

Natural abundance 2H NMR spectra of molecules oriented in liquid crystals.

It has been demonstrated that the natural abundance 2H NMR spectra of molecules dissolved in liquid crystals can be obtained easily with the help of present-day high-resolution FT NMR spectrometers. Such spectra obtained in the case of benzene and chloroform dissolved in liquid crystals are reported. These experiments could serve as valuable aids for the analysis of the complex proton NMR spectra of oriented molecules.

Benzene↗
PubMed1991

Fetal response to maternal ascariasis as evidenced by anti ascaris lumbricoides IgM antibodies in the cord blood.

The immune response of the fetus to maternal infection with Ascaris lumbricoides was studied by estimating IgG and IgM antibodies, using ELISA, in 28 paired samples of mother and cord blood. A. lumbricoides adult male, cuticle and unembryonated egg antigen was used. Toxocara canis larva antigen was used as a specificity control. Presence of IgG in the cord blood does not signify fetal response, but the presence of IgM antibodies does. IgM antibodies to Ascaris adult male antigen were present in 27 out of 28 maternal sera and 1 out of 28 cord sera. All maternal sera and only 6 cord sera showed IgM antibodies against Ascaris cuticle and 20 out of 22 maternal sera and 8 out of 22 cord sera tested, showed IgM antibodies to Ascaris unembryonated egg antigen. Sixteen out of 22 maternal sera and none out of 22 cord sera showed IgM antibody against Toxocara canis antigen, ruling out cross reaction and transplacental leak. The results of our study suggest that the Ascaris infection can spread to the fetus and elicit immune response.

Antibodies, Helminth↗
PubMed1989

Evidence for the presence of phosphoinositide cycle and its involvement in cellular signal transduction in the rabbit lens.

In the lens, free inositol is present at high concentrations. The lens transports inositol from the extracellular source but can also synthesize inositol from glucose via inositol-1-phosphate. The inositol containing phospholipid (phosphoinositides) constitutes only 10% of the total phospholipid in the membrane and was suggested to play some key role in the cellular differentiation. Recently, one of the phosphoinositides, PIP2, was located in the epithelial cells but not in fiber cells. Prostaglandin, which uses one of the phosphoinositide metabolites, diacylglycerol, as a precursor in its biosynthesis was also found in the lens. The evidence, although scanty, do provide some clues to the possibility that lens may contain a phosphoinositide cycle similar to retina and cornea. In this study we demonstrated that rabbit lens epithelial cells could incorporate 3H-inositol into the membrane and the label accumulated in all three phosphoinositides, PI, PIP and PIP2 with PI as the predominant form. Both PI Kinase and PIP Kinase were found in the lens epithelial homogenate which incorporated (gamma-32P) ATP into PI and PIP to form their respective product, PIP and PIP2. The membrane bound PI Synthase was also demonstrated by using a cell free system. The lens cells showed distinctive response to some agonists such as Ca2+, EGF, glucagon, serotonin but not the others such as insulin, FGF. It is therefore concluded that lens epithelium cells, like other cell types has a complete and functional phosphoinositide cycle.

1-Phosphatidylinositol 4-Kinase↗