[Bird breeder's lung. Epidemiological and immunological remarks apropos of 25 cases].
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Biomedical subjects
Publications and source records attributed to S Walbaum.
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An adult house mouse infected with an alveolar hydatid of Echinococcus multilocularis was trapped in the cellar of an inhabited house in a small village in Auvergne (France) where 3 human cases of alveolar hydatid disease had been diagnosed. The rodent harboured a young hepatic larval cyst. This indicated the presence of infective eggs of the parasite inside the house and outbuildings, an important observation concerning the circulation of the parasite in a domestic environment.
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Chlamydospore production by Candida albicans was investigated in rice cream decoction inoculated with a blastospore suspension. Effects of glucose, KH2PO4, (NH4)2SO4 and biotin addition were estimated using a "chlamydospore index" i.e. the mean-number of mature chlamydospores produced from a single blastospore development. If no other component was added, it appeared that better sporulation was obtained with both glucose concentration and inoculum size optimum (0.5 g/l and 7.8 X 10(3) blastospores/ml respectively). In glucose-KH2PO4 enriched medium, chlamydosporulation was the best with 0.5 g/l glucose and 0.5 g/l KH2PO4. The addition of biotin reduced the chlamydospore index at the concentrations tested (0.6 to 20 microgram/l). When glucose and (NH4)2SO4 were added together, it seemed that one of the C or N component had to be in low concentration for a good sporulation. The action of salts was also investigated; and finally a synthetic medium is proposed. The improvement of this medium is now in progress.
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Candida albicans is cultivated on rice cream decoction with glucose for chlamydospore production. A developmental stage refered as readiness is recognized. At this stage, the pseudomycelial thalli are able to produce chlamydospore in water without further stimulus by the sporulation medium. The greater the inoculum, the earlier appears the readiness. During the growth phase up to the readiness, the light is inhibiting the chlamydosporulation and favorising the yeast development. After the readiness, light still inhibits chlalmydospore formation.