PubMed Health⌕ Search

Biomedical subjects

S Watari

Publications and source records attributed to S Watari.

At least 19 recordsLinked to original sources
PubMed1988

Pharmacokinetics of an active cadralazine metabolite in plasma and blood vessels of spontaneously hypertensive rats.

The plasma and blood vessel (aorta and mesenteric artery) levels of (+/-)-6-[ethyl(2-hydroxypropyl)amino]-3-hydrazinopyridazine (ISF-2405), an active metabolite of cadralazine, were determined and compared with the changes in blood pressure in spontaneously hypertensive rats after single oral administration of cadralazine. The mean plasma level of ISF-2405 reached a maximum at 0.5 h after oral administration of 3 mg/kg of cadralazine, followed by a rapid elimination with a half-life of 1.0 h, whereas the mean aorta level of ISF-2405 reached a maximum at 4 h after dosing, followed by a slow elimination with a half-life of 6.5 h. The mean mesenteric artery levels of ISF-2405 were almost the same as the mean aorta levels over the time investigated. Both patterns of aorta and mesenteric artery levels of ISF-2405 were in good agreement with those of the hypotensive effects. These findings substantiate the assumption that the slow onset and long duration of the pharmacological effect of cadralazine is closely related to the distribution pattern of the active metabolite ISF-2405 in blood vessels, a target tissue of antihypertensive vasodilator drugs.

Animals↗
PubMed1987

Determination of ISF-2405, an active metabolite of cadralazine, in plasma and tissues by radioimmunoassay.

A radioimmunoassay has been developed which makes possible sensitive and specific determination of an active metabolite of cadralazine, (+/-)-6-[ethyl(2-hydroxypropyl)amino]-3-hydrazinopyridazine (ISF-2405), in plasma and tissues. On account of its lability in a sample solution, ISF-2405 in biological samples was selectively derivatized to a stable form, (+/-)-6-[ethyl(2-hydroxypropyl)amino]-3-(3,5-dimethyl-1-pyrazolyl) pyridazine (ISF-3349), by treatment with acetylacetone. The concentration of ISF-2405 was determined by the assay of the resultant ISF-3349. Antiserum against ISF-3349 was elicited in guinea pigs immunized with the ISF-3349 derivative coupled with bovine serum albumin. The obtained antiserum was highly specific for ISF-3349, and did not cross-react with either cadralazine or its metabolites. [Pyrazole-4-3H]labeled ISF-3349 with a specific activity of 13.9 Ci/mmol was used as the radioligand. Assays of ISF-2405 in plasma and tissues were possible over the concentration range from 0.4 to 6.4 ng/ml with 1 ml of plasma, and from 2 to 64 ng/g with 100 mg of tissue. Plasma and blood vessel levels of ISF-2405 in rats after single oral administration of cadralazine have also been determined by the present method.

Animals↗
PubMed1986

Lateral approach to the hook of hamate for its fracture.

We have recently experienced one case of the fracture of the hook of the hamate that was diagnosed only by the CT scan. As a surgical approach, lateral approach to the hook of the hamate for its fracture was utilized. We found that this approach was safe, easy and quick.

Adult↗
PubMed1985

New surgical procedure for correction of club hand.

Two cases of radial club hand were treated by a new surgical procedure. This procedure consisted of correction of the wrist deviation and pollicization. The metacarpal of the pollicized digit was used for radial osseous support.

Child↗
PubMed1984

Surgical treatment of cleft hand and its associated deformities.

Surgical procedures frequently used in the treatment of the cleft hand and its related deformities are presented. The need to pay attention to the particular stage of the developmental mechanism producing the deformity before undertaking surgery is stressed, for in addition to closure of the central cleft, treatment must include correction of the various symptoms associated with the finger ray fusion.

Child, Preschool↗
PubMed1984

[Sensitive determination of haloperidol in human serum by radioimmunoassay].

An improved procedure for the sensitive determination of the neuroleptic drug, haloperidol, in human serum is described. The method is based on a single hexane extraction procedure and a radioimmunoassay. Antiserum was elicited in guinea pigs immunized with haloperidol hemisuccinate derivative coupled to bovine serum albumin. Any appreciable cross-reactivity was observed neither with known metabolites of haloperidol nor with haloperidol decanoate (KD-136). Some devices in the extraction procedure, e.g. the use of 10 ml of extraction solvent, made it possible to measure haloperidol levels as low as 0.05 ng/ml. The accuracy and reproducibility of the method were shown to be sufficient. The present method can be used to study the pharmacokinetics of haloperidol in the phase 1 study of KD-136. In addition, it is simple enough for use in clinical laboratories that are monitoring haloperidol concentrations in the blood of psychiatric patients.

Evaluation Studies as Topic↗
PubMed1979

Experimental studies on the effects of tension on intraneural microcirculation in sutured peripheral nerves.

Tension on an end-to-end nerve suture causes injury to the intraneural vessels. Impairment of the transverse anastomoses occurs first, followed by degeneration of the intrafascicular vessels. The the transverse anastomoses undergo complete degeneration, and the extrafascicular vessels also sustain severe damage. This series of circulatory disturbances is an important factor to consider when determining the advisability of an end-to-end nerve suture.

Angiography↗