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S Yanagisawa

Publications and source records attributed to S Yanagisawa.

18 recordsLinked to original sources
PubMed1992

MNF1, a leaf tissue-specific DNA-binding protein of maize, interacts with the cauliflower mosaic virus 35S promoter as well as the C4 photosynthetic phosphoenolpyruvate carboxylase gene promoter.

When gel shift assays were performed with maize nuclear extract and a DNA fragment containing the cauliflower mosaic virus (CaMV) 35S promoter, three DNA-protein complexes were observed. Analyses with nuclear extracts prepared from green leaves, etiolated leaves, stems and roots showed that the complexes resulted from the existence of at least two nuclear factors. One of them is presumably a constitutive nuclear factor found in all tissues tested, and another is a leaf-specific factor present both in green and etiolated leaves. This leaf-specific nuclear factor seemed to be identical to MNF1, previously identified as a factor interacting with the promoter of the maize gene for phosphoenolpyruvate carboxylase involved in the C4 photosynthesis. Deletion analysis revealed that MNF1 binds to the sequence from -281 to -235 relative to the transcription start site of the CaMV 35S promoter. MNF1-like nuclear protein was also found in tobacco nuclear extracts. The possibility that MNF1 participates as a positive trans-acting factor in the expression of genes in maize leaves is discussed.

Base Sequence↗
PubMed1992

Molecular evolution of phosphoenolpyruvate carboxylase for C4 photosynthesis in maize: comparison of its cDNA sequence with a newly isolated cDNA encoding an isozyme involved in the anaplerotic function.

Molecular events associated with the evolution of an enzyme for C4 photosynthesis were investigated. In maize, at least three isozymes of phosphoenolpyruvate carboxylase [EC 4.1.1.31] are known: the C4-form, the C2-form and the root-form, being named according to their physiological roles and pattern of tissue distribution [Ting, I.P. & Osmond, C.B. (1973) Plant Physiol. 51, 448-453]. A cDNA clone which presumably encodes the C3-form isozyme was newly isolated and analyzed. Comparison of the sequences of the C3-form and C4-form isozymes revealed that (i) the homologies in the nucleotide and deduced amino acid sequences were 71 and 77%, respectively, and (ii) the gene for the C4-form isozyme evolved under strong G/C pressure. The genes for these isozymes were found to be located apart on different chromosomes. A phylogenetic tree was constructed using 8 amino acid sequences of phosphoenolpyruvate carboxylases from various sources. The topology of the tree indicated that, at least in monocots such as maize and sorghum, the genes for the C4-form and C3-form isozymes diverged from their common ancestral gene earlier than the monocot-dicot divergence (about 2 x 10(8) yr ago), though the divergence of maize (C4 plant) from wheat (C3 plant) is supposed to have occurred much later (6 x 10(7) yr ago).

Amino Acid Sequence↗
PubMed1992

[Histological evidence of prolonged coronary artery spasm in a necropsy case of recurrent myocardial infarction].

A 69-year-old male had acute inferior infarction during an operation of an aneurysm of the abdominal aorta and died from multiple organ failure 3 weeks after the operation. His initial coronary angiogram (CAG) showed no significant stenosis, but repeated CAG showed that the patients had developed a prolonged spasm in the mid-portion of the right coronary artery. Histologically, we observed eccentric luminal narrowing with atheromatous plaque where the spasms had been demonstrated, and a "waving form" of elastic fibers accompanied by intimal thickening in the opposite site of the atheromatous plaque. These findings suggest that organic stenosis may be underestimated angiographically because of compensatory enlargement of the non-atheromatous site in some patients with coronary artery spasm, and prolonged spasms may augment organic stenosis by following intimal thickening.

Aged↗
PubMed1990

Phosphoenolpyruvate carboxylase prevalent in maize roots: isolation of a cDNA clone and its use for analyses of the gene and gene expression.

In search of the gene family for phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] in C4 plants, we isolated from a maize root cDNA library a new cDNA clone that cross-hybridized with a cDNA for PEPC involved in the C4 photosynthesis (Yanagisawa et al. (1988) FEBS Lett. 229, 107-110). Alignment of the nucleotide sequence with that of the probe cDNA revealed the absence of sequence homology in the 3' non-coding region between the two cDNAs. Southern blot hybridization probed with this specific sequence indicated that the corresponding gene is unique in the maize genome. Northern blot hybridization using the same probe showed that this gene-family member was expressed most strongly in roots and also in green leaves to a lesser extent, but not significantly in etiolated leaves.

Base Sequence↗
PubMed1978

Partial distal trisomy 13q resulting from familial reciprocal 5/13 translocation.

A female infant with partial trisomy 13, 46,XX,der(5),t(5;13) (p15;q22)mat, for the distal part of the long arm is reported. The clinical and autopsy findings were similar to those of complete trisomy 13, except for harelip and cleft palate, and sloping forehead. Fetal hemoglobin and nuclear appendages in polymorphonuclear leukocytes were normal. Loci for these traits are discussed.

Abnormalities, Multiple↗
PubMed1978

Stature and body weight growth during adolescence based on longitudinal data of Japanese children born during World War II.

Longitudinal survey data of stature and body weight from age 7 to 17 were obtained for 100 boys and 100 girls during World War II. The growth rates and the average annual increments were compared with those of children born after the war. Growth attained at age 7 as a percentage of that at age 17 is larger in children of the control group, presumably as a result of an improved environment affecting the growth increment. The age at maximum velocity is six months to one year earlier for the current group of children. Although the maximum velocities for both items and sexes are nearly the same in the groups compared, the total increments are larger in the current group of children. Age, distance, and maximum velocity at adolescent growth spurt were obtained for each child. The mean values were compared according to growth patterns and growth attained at age 7. The "increasing type" growth group has the highest velocity at the greatest distance and the oldest age for stature. Children who were taller or heavier at age 7 have velocity peaks with greater distances.

Adolescent↗
PubMed1977

Stature and body weight growth patterns from longitudinal data of Japanese children born during World War II.

Stature and body weight data of 100 boys and 100 girls from 7 to 17 years of age in Shimodate City who were born during World War II were longitudinally analyzed. The children were significantly smaller and lighter throughout their growth period than those born 11 years after the end of the war. The correlation coefficient between statures at each age and at age 17 showed a gradual increase with increasing age, while that between statures at each age and at age 7 decreased with age. However, a drop in the correlation coefficient was found during puberty, at age 11 for girls and at age 13 for boys. Comparing the normalized distance from mean values of stature and body weight at age 7, at puberty, and at age 17, only 51% of the children continued to be in the same relative position for both height and weight, 6% of boys and 4% of girls showing a decreasing pattern for both and 4% of boys and 7% of girls showing an increasing pattern for both. Thus, about 60% of the children of either sex presented parallel stature and body weight growth patterns for ages from 7 to 17.

Adolescent↗