[Endoscopic therapy with adrenalin injection for bleeding due to diverticular disease of the colon].
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Aged↗
Biomedical subjects
S de las Heras
Publications and source records attributed to S de las Heras.
Considerations on the thalamostriatal system with some functional implications.
The thalamostriatal projections are largely neglected in current reviews dealing with basal ganglia function. In the past few years, however, several studies have re-evaluated these projections and have postulated their implication in more complex tasks within the basal ganglia organization. In this review, we try to focus on the morphological and functional importance of this system in the basal ganglia of the rat, cat and monkey. Special attention is paid to the thalamus as an important place for interaction between the input and the output systems of the basal ganglia through the thalamostriatal projections. Thus, we stress on the overlapping thalamic territories between the thalamic projection of the output nuclei of the basal ganglia and the thalamostriatal neurons. Our experimental data support the existence of several thalamic feedback circuits within the basal ganglia functional design. Finally, some considerations are provided upon the functional significance of these thalamic feedback circuits in the overall organization of the basal ganglia in health and disease.
Animals↗
Double retrograde tracer study of the thalamostriatal projections to the cat caudate nucleus.
The distribution of thalamostriatal neurons projecting to the cat caudate nucleus was examined by retrograde fluorescent tracers. Thus, Fast Blue and Diamidino Yellow were concomitantly injected in different rostrocaudal, dorsoventral, or mediolateral sectors of the caudate nucleus. The main findings of this study are as follows: (1) few double-labeled cells were found after two injections in different sectors of the caudate nucleus; (2) double-labeled neurons were more abundant after adjacent injections and they were mainly located in the caudal intralaminar nuclei, in the rhomboid nucleus and in the dorsal mediodorsal nucleus; and (3) there were variations in the spatial organization of the thalamostriatal neurons projecting to various sectors of the caudate nucleus in the different thalamic nuclei known to project to this part of the striatum.
Amidines↗
Thalamic interaction between the input and the output systems of the basal ganglia.
The striatal return through the thalamus is largely neglected in current studies dealing with basal ganglia function, and its role within this circuitry remains obscure. In this contribution the thalamus is regarded as an important place of interaction between the input and the output organization of the basal ganglia. In support of this idea, a brief overview is provided of some of the most recent findings concerning the thalamus in relation to the basal ganglia circuitry. In particular, we have focused on the thalamostriatal projections themselves, on the output of the basal ganglia to the thalamus and also on the overlapping territories between the thalamic projection of the output nuclei and the thalamostriatal neurons. These data support the existence of several thalamic feedback circuits within the basal ganglia neural system. Finally, some considerations are provided upon the functional significance of these thalamic feedback circuits in the overall organization of the basal ganglia.
Animals↗
Distribution of calbindin D-28k and parvalbumin neurons and fibers in the rat basal ganglia.
This review deals with the distribution of immunoreactivity for calbindin D-28k (CB) and parvalbumin (PV) in the different nuclei of the rodent basal ganglia analyzed with the data available after the use of single and double antigen procedures applied to single sections. These findings reveal that CB and PV are distributed according to a highly heterogeneous pattern in the caudate putamen complex (CPu), globus pallidus (GP), entopeduncular nucleus (EP), subthalamic nucleus (STh) and substantia nigra (SN) of the rat. In each basal ganglia structure, the two calcium-binding proteins label different neuronal subsets. Therefore, the use of CB and PV immunohistochemistry may be considered as an excellent tool to define distinct chemoarchitectonic and functional domains within the complex organization of the basal ganglia. Double immunohistochemical methods are also useful to illustrate the relationships between the different chemical subdivisions of the CPu, GP, EP, STh and SN and the chemically characterized connections with each other and with other forebrain and brainstem structures. However, specific rules should be followed when combining single and double immunostaining procedures, and the results of such studies must be evaluated with caution. When they are used properly, these methods can reveal hitherto unknown principles of organization of the basal ganglia and thus shed new light on the anatomical and functional organization of this set of subcortical structures involved in the control of motor behavior.
Animals↗
Overlapping territories between the thalamostriatal and nigrothalamic projections in cats.
Fluorescent tracers were injected into different regions of the caudate nucleus and HRP-WGA in the substantia nigra of the cat in order to analyse the thalamic distribution of retrogradely labelled thalamostriatal neurones and anterogradely labelled nigrothalamic terminals within the thalamus. Overlapping thalamic territories between the thalamostriatal neurones projecting to areas of the caudate nucleus and the nigrothalamic connections were observed in the rostral nuclei of the central thalamic group (ventral anterior nucleus, ventral anterior-ventral lateral complex and ventral medial nucleus) and, more restricted, in the rostral (rhomboid, paracentral, ventral lateral, dorsal mediodorsal nuclei) and caudal intralaminar nuclei (centromedian-parafascicular complex). This study provides evidence of the existence of thalamic areas in which the input and output of the basal ganglia converge.
Animals↗
Overlapping territories between the thalamostriatal and nigrothalamic projections in cats.
Fluorescent tracers were injected into different regions of the caudate nucleus and HRP-WGA in the substantia nigra of the cat in order to analyse the thalamic distribution of retrogradely labelled thalamostriatal neurones and anterogradely labelled nigrothalamic terminals within the thalamus. Overlapping thalamic territories between the thalamostriatal neurones projecting to areas of the caudate nucleus and the nigrothalamic connections were observed in the rostral nuclei of the central thalamic group (ventral anterior nucleus, ventral anterior-ventral lateral complex and ventral medial nucleus) and, more restricted, in the rostral (rhomboid, paracentral, ventral lateral, dorsal mediodorsal nuclei) and caudal intralaminar nuclei (centromedian-parafascicular complex). This study provides evidence of the existence of thalamic areas in which the input and output of the basal ganglia converge.
Animals↗
Re-examination of topographic distribution of thalamic neurons projecting to the caudate nucleus. A retrograde labeling study in the cat.
The distribution of thalamic neurons projecting directly to the caudate nucleus (CN) was examined using the retrograde labeling method. Horseradish peroxidase conjugated with wheat germ agglutinin (HRP-WGA) or a fluorescent tracer (either Fast Blue (FB) or Diamidino Yellow (DY)) was injected into various parts of the CN. The main findings were as follows: (1) labeled neurons were distributed most densely in the intralaminar nuclei, midline thalamic nuclei and centre median-parafascicular complex, and less densely in the ventroanterior (VA), ventrolateral (VL) and ventromedial (VM) nuclei. (2) After injections into the rostral parts of the CN, a moderate number of retrogradely labeled neuronal cell bodies was observed in VA, VL and VM. However, only very few, if any, labeled neurons were found in these nuclei after injections into the caudal parts of the CN. (3) After injections into the most dorsolateral parts of the CN, labeled neurons were seen in the lateralmost part of the VA and VL. (4) Many retrogradely labeled neurons were consistently found in the lateral wing of the rhomboid nucleus after injections into the CN. (5) No clear topography was detected in the arrangement of labeled neurons in the rhomboid, dorsal mediodorsal, centrolateral or paracentral nuclei. (6) After injections into the rostral parts of the CN, the most prominent labeling was observed in the ventral part of the centre médian-parafascicular complex.
Amidines↗
[Neurosciences: an example of an interdisciplinary approach as an efficient strategy in the scientific investigation].
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Education, Medical↗
New data on the anatomical organization of the thalamostriatal projections.
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Acetylcholinesterase↗
A topographic re-evaluation of the nigrostriatal projections to the caudate nucleus in the cat with multiple retrograde tracers.
The anatomical organization of the cat nigrostriatal projections to the caudate nucleus was studied by retrograde tracer techniques. Horseradish peroxidase conjugated with wheat germ agglutinin and fluorescent retrograde tracers such as Fast Blue and Diamidino Yellow were injected concomitantly in different regions of the caudate nucleus. The distribution of single and double retrogradely labeled neurons was analysed in the substantia nigra pars compacta, substantia nigra pars lateralis, retrorubral area and adjacent ventral tegmental area. Adjacent sections processed for acetylcholinesterase were used as histochemical markers for the densocellular zone of the substantia nigra. The main findings of this study are: (1) The rostral caudate nucleus receives projections mainly from the caudal substantia nigra while the caudal caudate nucleus receives projections from all rostrocaudal levels of the substantia nigra. (2) The substantia nigra pars lateralis projects very specifically to the caudal caudate nucleus. (3) The ventral retrorubral area close to the medial lemniscus projects to all rostrocaudal levels of the caudate nucleus. (4) The rostral caudate nucleus receives projections mainly from the medial substantia nigra while more caudal sectors of the caudate nucleus receive projections from the medial and lateral substantia nigra. (5) A dorsoventral inversion of nigrostriatal projections from the medial substantia nigra pars compacta and the adjacent ventral tegmental area to the caudate nucleus was established. In contrast, we found zones within the retrorubral area projecting both to the dorsal and ventral caudate nucleus. (6) Distant injections of two different fluorescent tracers regarding both the dorsoventral and the rostrocaudal coordinates, yielded double-labeled neurons that were mainly located in the medial and caudal portions of the substantia nigra and in the ventral retrorubral area. However, the number of double-labeled neurons was higher after separated injections in the dorsoventral axis, suggesting that the collateralization to the caudate nucleus occurs mainly in the dorsoventral plane. (7) A clustering organization of nigrostriatal cells projecting to the caudate nucleus was detected mainly in the intermediate rostrocaudal part of the substantia nigra pars compacta and in the retrorubral area. The results of this comprehensive study on the cat nigrostriatal pathway to the caudate nucleus show novel findings on the anatomical organization of the nigrostriatal projections which might help the understanding of the complex architecture of nigral neurons projecting to the caudate nucleus in carnivores.
Amidines↗
Organization of thalamic projections to the ventral striatum in the primate.
Although thalamic projections to the dorsal striatum are well described in primates and other species, little is known about thalamic projections to the ventral or "limbic" striatum in the primate. This study explores the organization of the thalamic projections to the ventral striatum in the primate brain by means of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) and Lucifer yellow (LY) retrograde tracer techniques. In addition, because functional and connective differences have been described for the core and shell components of the nucleus accumbens in the rat and are thought to be similar in the primate, this study also explores whether these regions of the nucleus accumbens can be distinguished by their thalamic input. Tracer injections are placed in different portions of the ventral striatum, including the medial and lateral regions of the ventral striatum; the central region of the ventral striatum, including the dorsal part of the core of the nucleus accumbens; and the shell region of the nucleus accumbens. Retrogradely labeled neurons are located mainly in the midline nuclear group (anterior and posterior paraventricular, paratenial, rhomboid, and reuniens thalamic nuclei) and in the parafascicular thalamic nucleus. Additional labeled cells are found in other portions of the intralaminar nuclear group as well as in other thalamic nuclei in the ventral, anterior, medial, lateral, and posterior thalamic nuclear groups. The distribution of labeled cells varies depending on the area of the ventral striatum injected. All regions of the ventral striatum receive strong projections from the midline thalamic nuclei and from the parafascicular nucleus. In addition, the medial region of the ventral striatum receives numerous projections from the central superior lateral nucleus, the magnocellular subdivision of the ventral anterior nucleus, and parts of the mediodorsal nucleus. After injection into the lateral region of the ventral striatum, few labeled neurons are seen scattered in nuclei of the intralaminar and ventral thalamic groups and occasional labeled cells in the mediodorsal nucleus. The central region of the ventral striatum, including the dorsal part of the core of the nucleus accumbens, receives a limited projection from the midline thalamic, predominantly from the rhomboid nucleus. It receives much smaller projections from the central medial nucleus and the ventral, anterior, and medial thalamic groups. The shell of the nucleus accumbens receives the most limited projection from the thalamus and is innervated almost exclusively by the midline thalamic nuclei and the central medial and parafascicular nuclei. The shell is distinguished from the rest of the ventral striatum in that it receives the fewest projections from the ventral, anterior, medial, and lateral thalamic nuclei.
Animals↗
Organization of the striatal projections from the rostral caudate nucleus to the globus pallidus, the entopeduncular nucleus, and the pars reticulata of the substantia nigra in the cat.
This study explores the organization of the striatal projections from the rostral caudate nucleus to the output nuclei of the basal ganglia in the cat. Tracer deposits were stereotaxically injected in different dorsoventral, mediolateral, and rostrocaudal sectors of the head of the caudate nucleus using horseradish peroxidase (HRP) conjugated with wheat germ agglutinin (HRP-WGA) either alone or mixed with free HRP. After the injections, a detailed analysis of the terminal labeling was carried out within the globus pallidus (GP), the entopeduncular nucleus (Ep), and the substantia nigra (SN) pars reticulata (SNR). Our findings illustrate how different dorsoventral, mediolateral, and rostrocaudal parts of the rostral caudate nucleus project primarily to similarly positioned but spatially segregated parts of GP. The striatoentopeduncular pathway was also organized topographically, but there was overlapping by projections from different parts of the rostral caudate nucleus. Areas of topographical segregation and zones of overlap were detected in the organization of the striatal projections from the rostral caudate nucleus to SNR. These results raise the possibility of distinct striatal actions upon different sectors of the output nuclei of the basal ganglia and, indirectly, upon their targets in the thalamus and brainstem.
Animals↗