Biomedical subjects
Publications and source records attributed to S el-Hawaary.
Bacteriophages recovery was enhanced by the addition of Mg2+ divalent cation or when the raw sewage filtrate was adjusted to pH 4.5. On the other hand, when Mg2+ or Ca2+ divalent cations were added to the acidified sewage filtrate, all sensitive phase species were found to be phage-resistant.
All the species tested were sensitive to gamma radiation treatment, even at a dose as low as 0.001 Mrad. The spore-former Bacillus cereus (PCJ 213) proved to be more resistant than the rest of the tested species, while the Gram-negative organisms were more susceptible to irradiation treatment. The tolerance among those organisms is depicted by the descending order: Proteus vulgaris, irregular VI, Escherichia coli I, E. coli III, and Salmonella typhi-murium. Yet all the tested faecal streptococci species, with the exception of Streptococcus bovis, may be regarded as holding an intermediate position between the more resistant B. cereus (PCJ 213) and the more sensitive Gram-negative organisms.
Samples of Nile and Ismailia canal water were subjected to direct inoculation and incubation at elevated temperatures. The results obtained showed that the use of MacConkey broth medium was better than EC broth. In addition, the Direct MPN technique revealed to be suitable and superior by 90% of the examined samples. A total of 300 isolates was recovered from Direct MPN-technique and identified by using IMViC reactions plus Eijkman test. 96% of the isolates were E. coli proper and 4% irregular types.
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