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S van Dijk

Publications and source records attributed to S van Dijk.

23 records · Page 2Linked to original sources

Use of fast protein liquid chromatography in the purification of inhibin from bovine follicular fluid.

Inhibin from bovine follicular fluid was partly purified using affinity chromatography on immobilized Procion Red 3B, gel filtration on Sephadex G-25 and ion-exchange chromatography on the fast protein liquid chromatography system. Inhibin was subsequently characterized using preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and electroelution. Biological activity was associated with a protein with an apparent molecular weight of approximately 65 kD.

Animals↗

Changes of serum enzyme activities in cows with milk fat depression.

Serum activities of sorbitol dehydrogenase (SDH), lactate dehydrogenase (LDH), gamma-glutamyltranspeptidase (gamma-GT), and alkaline phosphatase (ALP) were measured before, during, and after milk fat depression in 9 cows fed a high-concentrate ration during 2 experiments. In 7 of the 9 cows, increases in serum SDH and LDH activities were observed during milk fat depression. The gamma-GT activity showed only moderate changes, whereas the ALP activity remained unchanged. During recovery from milk fat depression, decreases of SDH, LDH, and gamma-GT activities were found in nearly all cows. Association of this phenomenon with changes found in beef cattle, such as ruminal lesions and liver abscesses, are discussed.

Alkaline Phosphatase↗

[Results of treatment by rotation in left-sided abomasal displacement (author's transl)].

The results are given of the treatment of thirty cows with left-sided abomasal displacement by rotation, followed by intravenous injection of hypertonic sodium chloride solution and a calcium borogluconate solution. In addition, ammonium chloride was administered orally for a few days. The rations of the animals were gradually restored to normal levels. The purpose was to examine whether recurrences following rotation could be prevented by supplementary measures. Of these thirty animals, five did not respond to treatment because of complications that would otherwise not have reacted favourably to any form of treatment either. Correction of the abomasal displacement was successful in more than 90 per cent (23 animals) of the remaining five cows. However, abomasal displacement recurred in sixteen of these twenty-three animals (70%). Of these fourteen cows, two animals developed an abomasal torsion. It is concluded that the supplementary measures adopted will not prevent recurrences.

Abomasum↗