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SJ Kim

Publications and source records attributed to SJ Kim.

6 recordsLinked to original sources
PubMed2000

Embryo recovery and transfer for the production of transgenic goats from Korean native strain, Capra hircus aegagrus.

During the breeding season in Korea (September 1997 to April 1998), a goat embryo recovery and transfer program using a Korean native strain (Capra hircus aegagrus) was performed for the production of transgenic goats. Donors were synchronized with norgestomet implants and superovulated by a combined treatment with FSH and hCG. The treatment regime induced a consistent and efficient superovulation rate of 90% from donors with an ovulation rate of 12.1+/-0.5. 50.5% of the recovered oocytes/embryos were fertilized and most of them were at the 1-cell stage. After DNA microinjection, a total of 188 embryos were immediately transferred to naturally cycling or hormonally synchronized recipients with two or three embryos per animal. There was a tendency for the pregnancy rate of naturally cycling recipients to be higher (38.9%) than that of hormonally synchronized recipients (25.7%). When the recipients were classified into a two or three embryo-received group, the embryo viability was markedly decreased from 58.3% in two embryo-received group to 35.3% in three embryo-received group, without an increase in the kidding rate. This resulted from a high occurrence of abortions or stillbirths from the multiple-pregnant recipients which had received three embryos. This indicated that the transfer of two embryos per recipient is highly recommendable for an optimal embryo survival in Korean goats. Altogether, 188 embryos were transferred to 71 recipients and two transgenic Korean goats were produced from 25 offspring.

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PubMed2000

Cu(II) Sorption Mechanism on Montmorillonite: An Electron Paramagnetic Resonance Study.

The mechanism of Cu(II) sorption on montmorillonite was studied with electron paramagnetic resonance (EPR) spectroscopy. The major sorbed species were successfully identified by EPR spectroscopy as they showed distinct signals due to their strength of binding and local structure. The EPR results together with macroscopic sorption data show that the sorption involves at least three different mechanisms. The dominant sorption mechanism changed with pH and Na(+) content in solution. In the acidic pH range, the sorption is independent of pH but is dependent on Na(+) concentration. Like free copper ions, the sorbed Cu(II) shows an isotropic absorption line. It is thought that the Cu(II) is ion-exchanged in the interlayer site and is capable of free tumbling motion despite electrostatic binding force. In the near neutral pH range, the sorption is strongly pH dependent and the sorbed Cu(II) shows no EPR signal. It is interpreted that the Cu(II) is sorbed by a surface complexation mechanism to form an inner-sphere surface complex. The EPR spectrum with a dipolar splitting pattern means that the dimeric Cu(II) surface species is the dominant sorbed species in the basic pH region where the Cu-Cu internuclear distance of the dimer is estimated to be approximately 3.3 Å. Copyright 2000 Academic Press.

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PubMed1998

Local orbital forcing of antarctic climate change during the last interglacial

During the last interglacial, Antarctic climate changed before that of the Northern Hemisphere. Large local changes in precession forcing could have produced this pattern if there were a rectified response in sea ice cover. Results from a coupled sea ice-ocean general circulation model supported this hypothesis when it was tested for three intervals around the last interglacial. Such a mechanism may play an important role in contributing to phase offsets between Northern and Southern Hemisphere climate change for other time intervals.

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PubMed1997

Immunohistochemical Detection of P-glycoprotein in Breast Cancer and Its Significance as a Prognostic Factor.

Overexpression of P-glycoprotein (Pgp) in tumors is one of the major mechanisms which mediates the multidrug resistance (MDR) phenotype. To evaluate the prognostic significance of Pgp in breast cancer, Pgp expression was examined in paraffin-embedded tissue sections of 94 breast cancer specimens by immunohistochemistry. Tissue specimens were obtained by mastectomy without preoperative chemotherapy. UIC2 monoclonal antibody which recognizes an extracellular epitope of human Pgp was employed. Of the 94 breast cancer specimens, 35(37.2%)were positive for Pgp expression. Pgp expression had no correlation with menopausal or hormone receptor status, axillary Iymph node involvement or tumor size. However, a significant correlation was observed between Pgp expression and disease relapse (p=0.0322). Pgp-positive patients showed a significantly shorter disease-free survival period than Pgp-negative patients by the Kaplan-Meier method (p=0.0433). These results suggest that immunohistochemical detection of Pgp in breast cancer tissue may have prognostic value after radical operation.

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