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Sambit K Mohanty

Publications and source records attributed to Sambit K Mohanty.

At least 19 recordsLinked to original sources
PubMed2004

Cytologic diagnosis of ossifying fibromyxoid tumor of soft tissue: a case report.

Ossifying fibromyxoid tumor (OFMT) of soft tissue is an uncommon, recently described neoplasm. It is usually, but not universally, a benign neoplasm and cases of metastatic so-called "malignant OFMT" have been reported. Although the histopathologic features of OFMT are well characterized, the same is not entirely true so far as the cytology literature is concerned. There are only three cytologic descriptions of OFMT to date to the best of our knowledge. A 62-year-old woman presented with a subcutaneous well-circumscribed nodular soft tissue mass at the upper end of the shoulder. X-ray and computed tomography revealed an incomplete shell of calcification surrounding the nodular mass along with foci of ossification within it. Fine-needle aspiration (FNA) cytology showed round to polygonal to spindle shaped neoplastic cells arranged in clusters, cords, and small aggregates and lying discretely in a mucoid background. Some osteoid-like material was also seen. The tumor was completely excised and histopathologic examination confirmed the diagnosis of OFMT. OFMT should be considered as a possibility in FNA cytology of myxoid soft tissue tumor especially with radiologic evidence of ossification.

Biopsy, Fine-Needle↗
PubMed2004

Aging changes in the human brain: a histochemical and immunohistochemical study.

There are very few studies on neuropathological changes of aging in the Indian population. In the present study, the incidence of aging changes in 20 non-demented elderly humans above the age of 55 years were observed. The incidence of neurofibrillary tangles (NFT) increased from 20% in 50-55-year-olds to 80% in 55-year-olds and over. Senile plaques (SP) increased from 20% in 50-55-year-olds to 80% in 55-60-year-olds and 40% in 60-year-olds and over. The results of immunohistochemistry to label the NFT (Tau) and SP (beta-amyloid) were comparable to the Bielchowsky's modified silver methanamine method. The incidence of age related changes and their antigenic character in the limited sample studied from north-west India appear to be comparable to those from the southern part of India and Western hemisphere.

Aged↗
PubMed2004

Mean nuclear volume in ovarian papillary serous cystadenocarcinoma.

OBJECTIVE: To measure mean nuclear volume (MNV), estimated by the stereologic intercept method, in ovarian serous carcinomas and to compare that between omentum-positive cases (cases with omental metastasis) and -negative cases. STUDY DESIGN: This retrospective study consisted of 29 cases (stage I and II = 13 cases, and stage IIIb = 16 cases) of ovarian papillary serous cystadenocarcinoma. MNV of all cases (at primary and metastatic sites) was estimated with an image cytometer. The nuclear point intersection method was used to measure nuclear volume. Mean intercepted diameters of at least 50 nuclei were measured. MNV was correlated with histologic grade, International Federation of Gynecology and Obstetrics (FIGO) staging of ovarian malignancy and primary tumor versus metastatic deposits. RESULTS: MNVs estimated in omentum-negative (FIGO stage I and II) and omentum-positive (FIGO stage IIIb) cases were 1,022.48 +/- 608.45 and 2,152 +/- 1,317.51 microns 3 (P = .05, Student t test), respectively. Significant differences in MNV were also observed among the different grades of tumor. However, no significant difference was observed between MNVs of primary and metastatic tumors in omentum-positive cases. CONCLUSION: Estimates of MNV on conventional histopathology sections may provide objective and useful criteria for relatively subjective histopathology grading and staging (FIGO stage I and II vs. IIIb).

Cell Nucleus↗
PubMed2004

Mean nuclear volume in squamous cell carcinoma of the vulva.

OBJECTIVE: To compare mean nuclear volume (MNV) estimated by the stereologic intercept method in lymph node-positive and -negative cases of squamous cell carcinoma of the vulva. STUDY DESIGN: This retrospective study consisted of 53 cases (lymph node metastasis, n = 19; cases without lymph node metastasis, n = 34) of squamous cell carcinoma of the vulva. MNV was estimated with the help of an image cytometer. The nuclear point intersection method was used to measure MNV. The mean nuclear volumes of both lymph node-positive and -negative cases were compared. RESULT: MNV in the lymph node-negative and -positive cases was 717.0 +/- 533.1 and 1,961.4 +/- 1,369.6 microns 3, respectively (P < .000, Mann-Whitney U test). There was a significant difference in MNV between the 2 groups of tumors. CONCLUSION: The observations from the present study suggest that estimation of MNV of malignant squamous cells from the vulva on conventional histopathology sections may provide an objective and useful diagnostic tool in predicting lymph node metastasis.

Carcinoma, Squamous Cell↗
PubMed2003

Cytologic diagnosis of fibrous hamartoma of infancy: a case report.

Fibrous hamartoma of infancy (FHI) is a rare benign lesion in the first 2 years of life with a distinctive histomorphologic appearance. There were only two descriptions of this entity in the cytology literature. In this article, the detailed clinical profile and cytologic features of a case of FHI is described in a 6-month-old male infant who presented with a nodular mass in the anterior axillary fold. Fine-needle aspiration (FNA) cytology smears showed an admixture of adipose tissue fragments, discretely lying, and clusters of fibroblastic cells in association with myxoid and collagenous matrix. FNA cytology features along with proper clinical history, radiological findings, family history, and location of the lesion may be helpful to suggest the probable preoperative diagnosis of this lesion.

Axilla↗
PubMed2003

Fractal dimensions of breast lesions on cytology smears.

In the present study, box-counting fractal dimensions of benign and malignant cells of breast tumors on cytology material were measured and compared. We selected fine-needle aspiration cytology smears of 14 cases of histopathology-proven infiltrating duct carcinomas and 7 cases of fibroadenoma of the breast. Five cells were randomly selected from each case. Box-counting fractal dimensions of all cells were measured with the help of an image cytometer (Leica, Cambridge, UK), using Quantimet 600 software (Leica). In total, 70 malignant cells and 35 benign cells were studied. The mean fractal dimensions of malignant cells and benign cells were 0.9571 +/- 0.1265 and 0.8354 +/- 0.1367, respectively. There was significant difference in the fractal dimension of malignant and benign cells (P = 0.006, Mann-Whitney nonparametric test). The measurement of fractal dimension may be helpful in discriminating malignant from benign cells. This may be another discriminating feature of malignant cells, along with classic image morphometry based on Euclidean geometry.

Biopsy, Fine-Needle↗
PubMed2003

Progressive macular hemangioma: a new entity?

We report a 22-year-old man with an extensive cutaneous vascular lesion involving the left arm since the age of 2 months. The lesion was macular and had been growing steadily in extent since its appearance. It had crossed the midline in several places. There was no involvement of any internal organ. Histopathology and immunostaining revealed unmistakable evidence of vascular proliferation. The predominantly macular character of the lesion, even with passage of time, besides the absence of several other features, usually associated with a tufted angioma, led us to the use of the term 'progressive macular hemangioma' for this condition.

Adult↗
PubMed2003

Manual and automated AgNOR count in differentiating reactive mesothelial from metastatic malignant cells in serous effusions.

OBJECTIVE: To distinguish reactive mesothelial cells from malignant cells in serous effusions using manual and automated methods of enumeration of argyrophilic nucleolar organizer regions (AgNORs). STUDY DESIGN: In this prospective study, 38 samples of benign (19 cases) and malignant (19 cases) serous effusions were included. AgNOR stain was used in each case along with routine Papanicolaou stain. The smears were examined under an oil immersion objective, and AgNOR dots were counted by direct observation independently by 2 observers. Automated AgNOR counting and morphometry were performed with a Quantimet 600 image cytometer (Leica, Cambridge, England). At least 100 cells were counted in each case. The number of AgNOR dots in individual cells, AgNOR area, nuclear area, AgNOR vs. nuclear area and nuclear perimeter were measured. Data on benign and malignant cells were compared. RESULTS: The AgNOR dots were discrete and smaller in benign effusion cases as compared to coarse and aggregated in malignant effusion cases. In benign reactive effusion cases the mean number of AgNOR dots per nucleus was 2.33 +/- 0.71 and 2.83 +/- 1.15 by the manual and automated method, respectively, whereas that for malignant effusion cases was 7.48 +/- 2.51 and 8.09 +/- 1.69 by the manual and automated method, respectively. Mean total AgNOR areas in benign and malignant groups were 4.77 +/- 2.66 microns 2 and 38.22 +/- 13.71 microns 2, respectively. Mean nuclear area, nuclear perimeter and ratio of AgNORs vs. nuclear area were 48.72 +/- 19.30 microns 2, 24.68 +/- 10.25 microns and .098 in benign effusion cases as compared to 174.25 +/- 82.36 microns 2, 69.03 +/- 27.23 microns and 0.22 in malignant effusion samples. All these values were significantly higher (P < .001, Student's t test) in malignant cells as compared to benign reactive cells. CONCLUSION: AgNOR dot enumeration, AgNOR area and ratio of AgNORs to nuclear area are valuable adjuncts to cytomorphology in differentiating reactive mesothelial cells from malignant cells in serous effusions. Automated AgNOR counting is rapid and less cumbersome.

Adenocarcinoma↗
PubMed2003

Bone marrow metastasis in solid tumors.

Bone marrow examination has been increasingly useful in documenting metastatic involvement of tumors. A retrospective analysis of 73 cases of bone marrow metastasis of solid tumors revealed 27 cases in the pediatric age group and 46 cases in the adult age group. All 27 pediatric cases were that of neuroblastoma. In the adult bone marrow metastasis from carcinoma prostate were present in 22 cases followed by carcinoma breast in 13 cases. Rest were 5 cases of carcinoma lung, 4 cases of carcinoma colon, 1 case each of carcinoma thyroid and renal cell carcinoma. A number of associated features were observed which may help to suggest bone marrow metastasis, in the absence of tumor cells in the bone marrow.

Adolescent↗