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Sang Cheol Seong

Publications and source records attributed to Sang Cheol Seong.

13 recordsLinked to original sources
PubMed2006

Is the degree of osteophyte formation associated with the symptoms and functions in the patellofemoral joint in patients undergoing total knee arthroplasty?

Osteophyte formation and loss of articular cartilage are the main pathologic features of osteoarthritis in the patellofemoral joint. The goal of this study was to determine whether the degree of osteophyte formation was associated with the degree of cartilage loss and the patellofemoral symptoms and functions in osteoarthritic patients undergoing total knee arthroplasty (TKA). We evaluated 71 knees in 54 consecutive patients who underwent TKA under the diagnosis of osteoarthritis. The degree of patellofemoral osteophyte formation was assessed by proportional diameter and proportional rim involvement. The cartilage status was assessed by depth and size of the cartilage lesion. The patellofemoral symptoms and functions included anterior knee pain, abilities of chair-rising and stair-climbing and quadriceps muscle power. No significant association was found between the degrees of patellar or trochlear osteophyte formation with anterior knee pain or any of the patellofemoral functional parameters (P>0.05). The degree of osteophyte formation in the patella and the trochlea had a significant but weak correlation with the status of patellar cartilage and the status of the trochlear cartilage, respectively (correlation coefficient = 0.22-0.41 and P<0.05). This study indicates that physicians need to be cautious not to easily reach the conclusion that the patellofemoral symptoms and functional disabilities can be attributed to the presence of osteophyte in the patellofemoral joint in consulting patients with the patellofemoral radiographs or deciding on patellar resurfacing in TKA.

Aged↗
PubMed2006

Delayed recurrent hemarthrosis after staple fixation of tibial avulsion fracture of the posterior cruciate ligament: a case report.

This study reports a case of unusual vascular complication related to the staple fixation for the tibial avulsion fracture of the posterior cruciate ligament (PCL). The patient, who experienced recurrent hemarthrosis 12 months after staple fixation for the avulsion fracture of the PCL, was successfully managed by removing the staple and suturing the bleeding focus of the popliteal artery. Injury to the popliteal artery by the prominent staples could be the culprit causing the recurrent hemarthrosis. This type of delayed popliteal artery injury should be kept in mind in open reduction and internal fixation for the tibial avulsion fracture of the PCL.

Adult↗
PubMed2006

Intraobserver and interobserver reliability of the assessment of the patellar articular cartilage in osteoarthritic patients undergoing total knee arthroplasty.

This study was performed to investigate intraobserver and interobserver reliability of the intraoperative assessment of patellar cartilage status and the decision on patellar resurfacing based on the cartilage assessment in osteoarthritic patients undergoing total knee arthroplasty. Photographs of patellar cartilage were taken in 80 knees of 53 patients undergoing total knee arthroplasty. Three investigators assessed the depth and size of the cartilage lesion and made a decision on patellar resurfacing, using the photographs twice. Intraobserver and interobserver reliabilities were calculated with the kappa coefficient. Cartilage assessment had substantial intraobserver (kappa = 0.77 for depth and 0.71 for size) and moderate interobserver (kappa = 0.54 for depth and 0.45 for size) reliabilities. Decision on patellar resurfacing had almost perfect intraobserver and substantial interobserver reliabilities (kappa = 0.88 and 0.68, respectively). We found that intraoperative assessment of the patellar articular cartilage and the decision for patellar resurfacing are reliable.

Aged↗
PubMed2006

Degree of degeneration and chondroitinase ABC treatment of human articular cartilage affect adhesion of chondrocytes.

The aim of the present study was to investigate whether degree of degeneration and chondroitinase ABC treatment of articular cartilage affect adhesion of chondrocytes to its surface. Human articular cartilage explants were harvested from patients undergoing total knee joint replacement arthroplasty, cut into discs of 0.5-0.8 mm thick and 3.75 mm in diameter, and then divided into three groups according to the degree of degeneration: G0, normal; G1, superficial fissures, and G2, deep fissures. These discs were stored at 70 degrees C until use. Chondrocytes (3 x 10(5) cell/mL in 100 microL of medium) isolated from articular cartilage by enzymatic digestion were transferred to a well plate containing cartilage discs and cultured for a maximum of 21 days. The cultures were divided into one of six groups according to the degree of degeneration and treatment of chondroitinase ABC of the disc. The morphologies of the disc surface and of cultured chondrocytes were assessed histologically. Chondrocyte adhesion, proliferation, and synthesis of proteoglycan and protein were analyzed. Chondrocyte adhesion significantly increased on the discs with higher degrees of degeneration, that is, maximum adhesion in G2, G1, and G0, subsequently. However, adhesion was not significantly different among the three groups at day 3. Chondrocyte adhesion on the chondroitinase ABC-treated disc significantly increased in comparison with that on the nontreated discs with the same degree of degeneration. However, proliferation of chondrocytes and synthesis of glycosaminoglycan and protein were not significant, regardless of degree of degeneration or chondroitinase ABC treatment. The present study demonstrated that chondrocyte adhesion on articular cartilage disc increased along with its degree of degeneration and with treatment of chondroitinase ABC, but that proliferation and synthesis of matrix of the cultured chondrocytes were not significantly influenced by both of them.

Aged↗
PubMed2004

Effects of dehydroepiandrosterone on articular cartilage during the development of osteoarthritis.

OBJECTIVE: To investigate the in vivo effects of dehydroepiandrosterone (DHEA) on knee joints during the development of experimentally induced osteoarthritis (OA). METHODS: Twenty-two mature NZW rabbits underwent bilateral anterior cruciate ligament transection (ACLT) and received 0.3-ml intraarticular injections of DHEA (at a concentration of 100 microM in phosphate buffered saline) and control solution in the right and left knees, respectively, beginning 4 weeks after ACLT and continuing once weekly for 5 weeks. All animals were killed 9 weeks after surgery, and the knee joints were assessed by gross morphologic, histologic, histomorphometric, and biochemical methods. RESULTS: Gross morphologic inspection following India ink application showed that the right femoral condyles, which received DHEA, demonstrated less severe cartilage damage than did the contralateral condyles. The thickness, area, and roughness of the DHEA-treated femoral condyles provided evidence of a cartilage-protecting effect of DHEA following ACLT. These results were supported by gene expression analysis. Messenger RNA expression of a proinflammatory cytokine, interleukin-1beta, and catabolic enzymes, matrix metalloproteinases 1 and 3, was reduced in the cartilage of the DHEA-treated knee joints, and expression of tissue inhibitor of metalloproteinase 1 was increased. CONCLUSION: Results of the present study demonstrate a cartilage-protecting effect of DHEA during the development of OA following ACLT in a rabbit model.

Animals↗
PubMed2004

Outcome of anterior cruciate ligament reconstruction using quadriceps tendon autograft.

PURPOSE: The purpose of this study was to determine the outcome of anterior cruciate ligament (ACL) reconstruction using a quadriceps tendon autograft. TYPE OF STUDY: A case series of patients who had received arthroscopic ACL reconstructions using quadriceps tendon autograft was retrospectively evaluated. METHODS: Sixty-seven ACL reconstructions were evaluated at a mean of 41 months (range, 27 to 49 months). Clinical assessment was made using a modified Lysholm score, documentation of International Knee Documentation Committee (IKDC), the anterior knee pain questionnaire of Shelbourne and Trumper, and by KT-2000 arthrometric analysis. Isokinetic strength testing and radiographic assessments were also performed. RESULTS: Arthrometric analysis showed that 63 knees (94%) were graded A or B with a median laxity of 2 mm postoperatively. The Lysholm score improved postoperatively from 71 to 90 ( P < or = .05). Extension peak torque of the quadriceps muscle recovered to 82% and 89% of that of the contralateral knee at 180 degrees/second at 1 year and 2 years after surgery, respectively. The patellar position in terms of congruence angle and Insall-Salvati ratio did not show any significant change. Only 4 patients complained of moderate pain on kneeling and 1 patient complained of harvest-site tenderness. CONCLUSIONS: ACL reconstruction using a quadriceps tendon autograft showed satisfactory results with reduced donor-site morbidities. The quadriceps tendon can be a reliable source of graft, and is comparable to bone-patellar tendon-bone or hamstring tendon in ACL reconstruction. LEVEL OF EVIDENCE: Level IV, Case Series (no, or historical, control group).

Adolescent↗
PubMed2004

Effects of a chitosan scaffold containing TGF-beta1 encapsulated chitosan microspheres on in vitro chondrocyte culture.

The objectives of this study were (1) to develop a three-dimensional chitosan scaffold in combination with transforming growth factor-beta1 (TGF-beta1)-loaded chitosan microspheres and (2) to evaluate the effect of the TGF-beta1 release on the chondrogenic potential of rabbit chondrocytes in the scaffolds. TGF-beta1 was loaded into chitosan microspheres using an emulsion-crosslinking method, resulting in spherical shapes with a size ranging from 0.3 to 1.5 microm. Controlled release of TGF-beta1, as measured by enzyme-linked immunosorbent assay (ELISA), was observed with chitosan microspheres over 7 days. Chitosan solutions (2% and 3%) were fabricated into two types of scaffolds with different pore morphologies and mechanical properties using a freeze-drying technique, with the result that scaffold with higher concentrations showed smaller pores and lower porosity, leading to a much stronger scaffold. The TGF-beta1 microspheres were incorporated into the scaffolds at a concentration of 10 ng TGF-beta1/scaffold and then chondrocytes seeded into each scaffold and incubated in vitro for 2 weeks. The 2% chitosan scaffolds showed higher cell attachment levels than the 3% chitosan scaffolds (P < 0.01), regardless of the TGF-beta1 microspheres. Both the proliferation rate and glycosaminoglycan (GAG) production were significantly higher for scaffolds incorporating TGF-beta1 microspheres than for the control scaffolds without microspheres 10 days after incubation. Extracellular matrix staining by Safranin O and immunohistochemistry for type II collagen both significantly increased in scaffolds containing TGF-beta1 microspheres. These results suggest that the TGF-beta1 microsphere incorporated in scaffolds have the potential to enhance cartilage formation.

Animals↗
PubMed2003

Tibial tunnel area changes following arthroscopic anterior cruciate ligament reconstructions with autogenous patellar tendon graft.

We investigated radiographic changes in tibial tunnel area after ACL reconstructions with autogenous patellar tendon grafts on anteroposterior and lateral radiographs over 3 years. Fifty patients followed up for at least 1 year were included in the study. Radiographs were taken on the day of surgery and 3, 6, 9, 12, 24, and 36 months postoperatively. Tibial tunnels on both radiographs were divided into proximal, middle, and distal one-third. The area of each one-third and the greatest diameter of the tibial tunnel on both radiographs was measured using an image-processing software. According to the tunnel area changes, the shape of tibial tunnel was classified into one of four shapes; cylinder, mallet, reverse bottle, and reverse triangle. The correlations between area, diameter and shape of the tunnel, and clinical variables including arthrometer measurement and clinical score were determined. The areas of each one-third of the tibial tunnels on lateral radiographs was always greater than that on anteroposterior radiographs, although the diameters on the two radiographs did not differ significantly. The area of proximal one-third largest and that of distal one-third smallest on both radiographs at any time point. The enlargement and reduction occurred within 3 months and tended to continue for 9 months. Thereafter the tunnel change stabilized on both radiographs. The most common shape of the enlarged tunnels was cylindrical on anteroposterior radiographs reverse triangle on lateral radiographs. No negative effects of enlarged area, diameter, or tunnel shape on clinical results were found in our study.

Adolescent↗
PubMed2003

Analysis of initial fixation strength of press-fit fixation technique in anterior cruciate ligament reconstruction. A comparative study with titanium and bioabsorbable interference screw using porcine lower limb.

We performed a controlled laboratory study to evaluate the initial fixation strength of press-fit technique. Forty porcine lower limbs were used and divided into four groups according to the method of fixation; group 1 (press-fit+1.4 mm), in which the diameter difference between the bone plug and the femoral tunnel was 1.4 mm; group 2 (press-fit+1.4 mm, 30 degrees), in which the diameter difference was the same with group 1, but the tensile loading axis was 30 degrees away from the long axis of the femoral tunnel; group 3 (titanium), in which a titanium interference screw was used for fixation; group 4 (bioabsorbable), in which a bioabsorbable interference screw was used for fixation. The graft in the press-fit group was harvested with a hollow oscillating saw with inner diameter of 9.4 mm to obtain consistent and completely circular shape of the bone plug. The femoral tunnel with diameter of 8 mm was drilled at the original ACL insertion. Following the bone plug insertion into the femoral tunnel and applying a preload of 20 N, the specimen underwent 500 loading cycles between 0 and 2 mm of displacement. Thereafter the specimen was loaded to failure. There was no fixation site failure during the cyclic loading test. Significant differences in the stiffness, linear load, or failure mode among the groups were not found. The average ultimate failure load of group 1 and group 2 were not significantly different from those of group 3 and group 4. The press-fit groups demonstrated sufficient fixation strength for the rehabilitation and interference screw groups. The completely circular shape of the bone plug and increased diameter difference between the bone plug and the femoral tunnel seemed to contribute to the strong fixation.

Animals↗
PubMed2003

Posterolateral reconstruction using split Achilles tendon allograft.

Injury to the cruciate ligaments of the knee commonly occurs in association with posterolateral instability, which can cause severe functional disability including varus, posterior translation, and external rotational instability. Failure to diagnose and treat an injury of the posterolateral corner in a patient who has a tear of the cruciate ligament can also result in the failure of the reconstructed cruciate ligament. Unlike isolated posterior cruciate ligament injury, there seems to be a consensus of opinion that injury to the posterolateral corner, whether isolated or combined, is best treated by reconstructing the posterolateral corner along with the coexisting cruciate ligament injury, if combined. Commonly proposed methods of reconstructing the posterolateral corner have focused on the reconstruction of the popliteus, the popliteofibular ligament, and the lateral collateral ligament. We introduce a new technique for reconstructing the posterolateral corner using a split Achilles tendon allograft. Our method reasonably addresses the several pitfalls in the reconstruction of the posterolateral corner, including (1) concurrent reconstruction of important posterolateral structures, (2) regaining the isometry of the lateral collateral ligament, (3) repositioning the reconstructed popliteus into its original position, and (4) providing a secure fixation method.

Achilles Tendon↗
PubMed2003

Clinical efficacy and safety of Lyprinol, a patented extract from New Zealand green-lipped mussel (Perna Canaliculus) in patients with osteoarthritis of the hip and knee: a multicenter 2-month clinical trial.

OBJECTIVE: To validate the clinical efficacy and safety of Lyprinol (a patented extract from Perna Canaliculus), a 5-LOX inhibitor in patients with osteoarthritis. METHODS: In this multicenter trial, 60 patients with symptomatic osteoarthritis of the knee and hip were included to receive Lyprinol at a dose of 2 capsules twice a day. After a 4- and 8-week treatment period, the following parameters were analyzed: visual analogue scale; Lequesne functional index; global assessment by patients; global assessment by physician; and adverse effects. RESULTS: Lyprinol treatment led to significant improvement of the signs and symptoms of osteoarthritis as determined by all efficacy measures. After a 4- and 8-week treatment period, 53% and 80% (respectively) of patients experienced significant pain relief, and improvement of joint function. There was no reported adverse effect during this clinical trial. CONCLUSION: Lyprinol was very effective and is a promising anti-inflammatory product that relieves the signs and symptoms of osteoarthritis, without adverse effect.

Aged↗
PubMed2002

Alternative splicing of type II procollagen gene in the dedifferentiation of rat epiphyseal chondrocytes serially cultured in monolayer.

Type II procollagen is synthesized in two forms (IIA and IIB), generated by the alternative splicing of its precursor mRNA. The purpose of this study was to determine whether alternative splicing of type II procollagen pre-mRNA occurs during the dedifferentiation of chondrocytes in monolayer culture. We serially cultured rat epiphyseal chondrocytes for eight weeks in monolayer, and investigated the expression patterns of the two forms of type II procollagen transcripts according to the duration of the culture period. Reverse transcription-polymerase chain reaction and northern analysis revealed that both transcripts peaked on the day of cell isolation, but the expression level of IIB form was much higher than that of IIA form. The expression of IIA form decreased gradually, whereas the expression of IIB form decreased so rapidly that the ratio of type IIB and type IIA transcripts was reversed in the second week. In situ hybridization showed that all cells expressed type IIA mRNA throughout the culture period. The signal intensity for type IIA transcript decreased gradually in accordance with the results of RT-PCR and northern analysis. In situ hybridization of type IIB mRNA showed different results from in situ hybridization of type IIA mRNA. Like type IIA transcript, the freshly isolated chondrocytes and the cells in the first week showed positive signals for type IIB transcript. However, in the second week, positive signal was detected neither in polygonal cells nor large fibroblastic cells. Only a few cells of small size were weakly positive. From the third to the eighth week, no positive signal was detected. These findings indicate that alternative splicing of type II procollagen gene does occur and is regulated during the dedifferentiation of chondrocytes in monolayer culture.

Alternative Splicing↗