Biomedical subjects

Sean J Kirkpatrick

Publications and source records attributed to Sean J Kirkpatrick.

4 recordsLinked to original sources

PubMed2006

Development of a reinforced porcine elastin composite vascular scaffold.

Elastin, a principal structural component of native arteries, has distinct biological and mechanical advantages when used as a biomaterial; however, its low ultimate tensile strength has limited its use as an arterial conduit. We have developed a scaffold, consisting of a purified elastin tubular conduit strengthened with fibrin bonded layers of acellular small intestinal submucosa (aSIS) for potential use as a small diameter vascular graft. The addition of aSIS increased the ultimate tensile strength of the elastin conduits nine-fold. Burst pressures for the elastin composite vascular scaffold (1,396 +/- 309 mmHg) were significantly higher than pure elastin conduits (162 +/- 36 mmHg) and comparable to native saphenous veins. The average suture pullout strength of the elastin composite vascular scaffolds was 14.612 +/- 3.677 N, significantly higher than the pure elastin conduit (0.402 +/- 0.098 N), but comparable to native porcine carotid arteries (13.994 +/- 4.344 N). Cyclic circumferential strain testing indicated that the composite scaffolds were capable of withstanding physiological loading conditions for at least 83 h. Implantation of the elastin composites as carotid interposition grafts in swine demonstrated its superiority to clinically acceptable ePTFE with significantly longer average patency times of 5.23 h compared to 4.15 h. We have developed a biologically based elastin scaffold with suitable mechanical properties and low thrombogenicity for in vivo implantation, and with the potential for cellular repopulation and host integration reestablishing an appropriate elastic artery.

Animals↗

PubMed2005

A primer on radiometry.

Correctly measuring radiometric quantities and reporting radiometric data is essential in developing a more complete understanding of the requirements and polymerization kinetics of dental resin composites. Presented herein is an introduction to the fundamental concepts, quantities and units of radiometry. A brief discussion of photometry and how it differs from radiometry is also included. While the basic principles of radiometry are relatively straightforward, a great deal of attention must be paid to the details when performing radiometric measurements and reporting radiometric data. Meaningful radiometric measurements require very close attention to nomenclature and mathematical definitions. Radiometric, not photometric quantities should always be used, unless the goal is to report how the human eye perceives light.

Composite Resins↗

PubMed2003

Acousto-optical characterization of the viscoelastic nature of a nuchal elastin tissue scaffold.

A nondestructive, acousto-optical method for characterizing the mechanical loss factor of biological tissues and tissue scaffolds is presented and applied to the characterization of an elastin tissue scaffold derived from bovine nuchal ligament. The method relies on launching guided surface acoustic waves into the tissue scaffold with a small speaker and simultaneously illuminating a small region of the scaffold distant from the speaker with a low-power HeNe laser. The phase lag between the driving acoustic wave and the shift in the backscattered laser speckle pattern is determined as a measure of the mechanical loss factor of the scaffold, tan delta. Measurements of tan delta and elastic modulus were also made by traditional dynamic mechanical loading techniques. Through the central portion of the loading cycle, the elastic modulus of the elastin scaffold was 1.2 x 10(6) +/- 1 x 10(5) N x m(-2) (parallel to fiber orientation). The estimated value of tan delta in the direction parallel to the elastin fibers was 0.03 +/- 0.017 by traditional methods and 0.029 +/- 0.03 when using the acousto-optical method. In the direction perpendicular to fiber orientation, tan delta was measured as 0.14 +/- 0.056 by the acousto-optical method. Because of a lack of mechanical integrity, it was not possible to measure tan delta in the direction perpendicular to fiber orientation by traditional methods. The acousto-optical method may prove to be useful in the mechanical characterization of developing engineered tissues.

Acoustics↗

PubMedDate not supplied

Low-frequency surface wave propagation and the viscoelastic behavior of porcine skin.

A physical model describing the propagation of low-frequency surface waves in relation to the viscoelastic behavior of porcine skin is presented, along with a series of empirical studies testing the performance of the model. The model assumes that the skin behaves as a semi-infinite, locally isotropic, viscoelastic half-space. While the assumption of a semi-infinite body is violated, this violation does not appear to have a significant impact on the performance of the model based on the empirical studies. 1-Hz surface waves in the skin propagate primarily as Rayleigh waves with a wavelength and velocity of approximately 3 m and 3.0 m/s, respectively. The amplitude of the acoustic wave, as measured by tracking the acoustic stress wave-induced shift in a backscattered laser speckle pattern, decreases exponentially with lateral distance from the acoustic source. Using this model of surface wave propagation, the mechanical loss factor or tan delta of the skin is measured to be on the order of 0.14+/-0.07. The results presented are consistent with earlier works on the propagation of low-frequency acoustic waves in biological tissues, and should serve as a theoretical and empirical basis for using the wave characteristics of propagating surface waves in combination with the mechanical behavior of the tissue for biomechanical studies and for potential diagnostic applications.

Acoustics↗