Sean T Corbett | PubMed Health

PubMed2006

Vitamin D receptor found in human sperm.

OBJECTIVES: Several animal studies have suggested that vitamin D and the vitamin D receptor (VDR) play a role in male fertility. The mechanism of action in the testis and the interaction with sperm is unknown. The presence of the VDR on sperm has never been demonstrated. The objective of this pilot study was to investigate the presence of the VDR on human sperm. METHODS: A prospective study of sperm collected from 10 fertile men, mean age 33.7 +/- 2.2 years, was undertaken. Qualitative analysis for VDR was performed by immunohistochemistry using a monoclonal antibody to human VDR. For comparison of the spatial relationship of the receptor, qualitative analysis of the androgen receptor on sperm was performed. Immunoprecipitation and immunoblotting of total sperm protein lysate using VDR antibodies further characterized the VDR. RESULTS: Immunohistochemistry demonstrated that the VDR was located predominantly on the head/nucleus of the sperm and mid-piece. Immunoblotting confirmed the presence of the VDR with a molecular weight of 50 kDa in all subjects. CONCLUSIONS: Our results have demonstrated the presence of the VDR on human sperm for what we believe to be the first time.

Adult↗

PubMed2006

Voiding ability using propofol sedation in children undergoing voiding cystourethrograms: a retrospective analysis.

PURPOSE: The ability of a child to void during cystourethrography is important in detecting vesicoureteral reflux. The potential effect of sedation on the capacity to void may impair our ability to detect vesicoureteral reflux. Since 2001, most voiding cystourethrograms at our institution have been performed with moderate sedation using propofol. To assess the impact of sedation on the ability of children to void, we retrospectively evaluated a group of patients who underwent sedated voiding cystourethrograms and compared them to a group undergoing nonsedated voiding cystourethrograms. MATERIALS AND METHODS: The nonsedated group consisted of children 2 to 8 years old who underwent voiding cystourethrography between 1996 and 2001. The sedated group consisted of children the same age who underwent voiding cystourethrography between 2002 and 2004. Patient characteristics, presenting symptoms, bladder capacity, emptying ability and diagnoses were recorded. Children were categorized as receiving sedation vs not receiving sedation. All sedated children received propofol deep sedation. Statistical analyses were performed using the 2-sided t test and Fisher's exact test. RESULTS: Of 544 charts reviewed 287 were within the age range defined. Of these children 85% were female. Sex was evenly matched between the sedated and nonsedated groups. Urinary tract infections (65%) and previous vesicoureteral reflux (25%) were the most common factors prompting voiding cystourethrography. Sedation was administered in 146 patients, of whom 80 (55%) were able to void to completion. Of the 141 patients who did not receive sedation 125 (89%) were able to void to completion (p <0.001). CONCLUSIONS: Children who underwent voiding cystourethrography with sedation were less likely to void to completion. This finding may impair our ability to detect vesicoureteral reflux in children accurately. Large prospective studies are needed for better assessment of bladder emptying and sedation when performing voiding cystourethrography.

Child↗