Biomedical subjects

Sergio Caputi

Publications and source records attributed to Sergio Caputi.

25 records · Page 2Linked to original sources

PubMed2004

Calcium sulfate: analysis of MG63 osteoblast-like cell response by means of a microarray technology.

Calcium sulfate (CaS) is an highly biocompatible material that has the characteristic of being one of the simplest as well as one of the synthetic bone-like graft with the longest clinical history, spanning more than 100 years. Solidified or crystallized CaS is very osteogenic in vivo. As the surface CaS dissolves in body fluid, the calcium ions form calcium phosphate that reprecipitates on the surface forming an osteoblast "friendly" environment. How this "friendly" environment alters osteoblast activity to promote bone formation is poorly understood. We therefore attempted to address this question by using microarray techniques to identified genes that are differently regulated in osteoblasts exposed to CaS. By using DNA microarrays containing 19,200 genes, we identified in osteoblast-like cells line (MG-63) cultured with CaS (Surgiplaster, Classimplant, Roma, Italy) several genes that expression was significantly upregulated. The differentially expressed genes cover a broad range of functional activities: (a) immunity, (b) lysosomal enzymes production, (c) cell cycle regulation, (d) and signaling transduction. It was also possible to detect some genes whose function is unknown. The data reported are, to our knowledge, the first genetic portrait of CaS effects. They can be relevant to better understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

Calcium Sulfate↗

PubMed2004

Genetic profiling of granular cell myoblastoma.

Granular cell tumor (GCT), or granular cell myoblastoma, is a relatively uncommon lesion of the soft tissues. It can occur in any organ, and the tongue is more often affected. GCT has unknown etiology, uncertain histogenesis, and a not always benign nature. Benign myoblastomas are the great majority, but rare malignant lesions have been reported. To have more information regarding the genetic events involved in GCT, the authors decided to perform an expression profile. A sample was derived from a surgically resected GCT of the tongue. RNA extracted from normal tongue (mucosa plus muscle) was used as control. By using DNA microarrays containing 19,200 genes, the authors identified several genes for which expression was significantly up- or down-regulated. The differentially expressed genes cover a broad range of functional activities: (1) signal transduction, (2) cell cycle regulation, and (3) cytoskeleton organization. It was also possible to detect some genes whose function is unknown. The data reported are, to the authors' knowledge, the first genetic portrait of GCT. Mutations in some of the described genes are related to neural alterations and mental diseases, and this fact supports the idea of a neural origin of myoblastoma. Several markers have been identified that will help in identifying the biological behavior (when malignant lesions will be described), as well as the gene whose products could be potentially disease-specific targets for therapy.

Cell Cycle Proteins↗

PubMed2004

Bacterial adhesion on commercially pure titanium and zirconium oxide disks: an in vivo human study.

BACKGROUND: Little is known about the mechanisms of bacterial interaction with implant materials in the oral cavity. A correlation between plaque accumulation and progressive bone loss around implants has been reported. Bacterial adhesion shows a direct positive correlation with surface roughness. Other surface characteristics also seem to be extremely important with regard to plaque formation. Different adhesion affinities of bacteria have been reported for different materials. The aim of this study was to characterize the percentage of surface covered by bacteria on commercially pure titanium and zirconium oxide disks. METHODS: Ten patients participated in this study. A removable acrylic device was adapted to the molar-premolar region, and commercially pure titanium (control) and zirconium oxide (test) disks were glued to the buccal aspect of each device. The surface roughness of titanium and test specimens was similar. After 24 hours, all disks were removed and processed for scanning electron microscopy, for the evaluation of the portion of surface covered by bacteria. RESULTS: In control specimens, the area covered by bacteria was 19.3% +/- 2.9; in test specimens, the area was 12.1% +/- 1.96. The disk surface covered by bacteria on test specimens was significantly lower than that of control specimens (P = 0.0001). CONCLUSION: Our results demonstrate that zirconium oxide may be a suitable material for manufacturing implant abutments with a low colonization potential.

Adult↗

PubMed2003

Adherence of investment to Au-Pd-Ag alloy using a vacuum-argon pressure casting machine.

OBJECTIVE: The removal of adhered investment material from marginal metal surfaces represents a possible risk of poor marginal adaptation. The aim of this work was to evaluate the effects of argon on investment adherence to the casting surface and on the alloy surface microstructure. METHODS: A die of stainless steel was used. 10 wax copings were cast by vacuum-argon pressure, while 10 copings by vacuum-air pressure using a Combilabor CLG-77 A in a graphite crucible; supply was direct with curved sprues of slashed circle 3.5 mm. The alloy used was Herabond. After coarse investment, removal of all specimens were investigated with scanning electron microscopy and semi-quantitative digital densitometry. RESULTS: The specimens cast under vacuum-argon pressure showed less amount of investment adhered (unpaired t test of grouped specimens, difference 2.896x10(5)pixel; p=0.002) while the grain size on the casting surface increased significantly p=0.000 (difference 5.968(microm). SIGNIFICANCE: Vacuum-argon pressure supply during casting reduces the investment adherence to the metal surface, and increases the packing density and size of the grains, especially on the casting surface. These minimize the risk of marginal distortions due to casting cleaning procedures and facilitate the finishing and polishing procedures.

Adhesiveness↗

PubMed2003

Ability of healthy and inflamed human dental pulp to reduce hydrogen peroxide.

This study examined the defensive ability of human dental pulp against H2O2 in healthy and reversible and irreversible pulpitis tissues through determination of catalase activity by spectrophotometric methods. Thirty-five systemically healthy patients were donors of the pulp tissue, and pulp conditions were assessed using clinical and X-ray evaluations. Catalase activity was 1.61 +/- 0.23 U mg(-1) protein in the healthy tissues, 2.99 +/- 0.45 U mg(-1) protein in the reversible pulpitis tissues, and 2.44 +/- 467 mU mg(-1) protein in the irreversible pulpitis tissues. All differences between the groups were statistically significant. These results point to a role for catalase during dental pulp inflammation in humans, and therefore demonstrate an inherent biological defense system against reactive oxidants in human dental pulp.

Adult↗

PubMed2003

A positioning device for computed tomography: a clinical report.

This clinical report describes a device (Centrascan) used to assist in the correct alignment of the patient's head during computed tomography (CT) assessment of a proposed implant site. To obtain the desired anatomic detail, CT requires precise alignment of the axial images at right angles to the long axis of the proposed implant. This clinical report compared the anatomic morphology of a projected implant site derived from axial images provided by DentaScan software analysis of the CT scan acquisition. Images from a conventional scan, with the patient's head aligned along the frontal plane (perpendicular to the Frankfort plane) and along the sagittal plane (coinciding with either the cortical bone of the hard palate or the inferior border of the mandible), were compared with images acquired by use of the Centrascan device. The two scans differed substantially. In particular, the cross-sectional images obtained by the conventional procedure showed a distorted anatomy; conversely, the images obtained by the Centrascan procedure showed a better reproduction of the examined area. The Centrascan device seemed to help the radiologist achieve a more correct alignment of the patient's head during CT scan acquisition. Further studies are necessary to fully explore the relative technical merits of the Centrascan device.

Dental Implants↗

PubMed2003

Bacterial adhesion on titanium nitride-coated and uncoated implants: an in vivo human study.

Titanium nitride (TiN) has been used in many fields as a surgical instrument coating that makes the surgical materials more resistant to wear and corrosion. The aim of the present study was an in vivo evaluation of the bacterial adhesion to TiN-coated (test) and uncoated (control) titanium implants. Six patients aged between 21 and 25 years and in excellent systemic health participated in the study. All of the participants gave their informed consent. The participants were selected on the basis of good periodontal health and no signs of mouth breathing. In each of the 6 participants, a removable acrylic device was adapted to the molar-premolar region of each quadrant of the jaws. One 4 x 13 mm titanium implant was glued to the buccal aspect of each device. The plasma spray covered 11.5 mm of the body of the implant, whereas the neck was machined titanium. Test implants were glued to the right devices and control implants were glued to the left devices. After 24 hours, the implants were removed from each device and processed for scanning electron microscopy for evaluation of the machined portion of the implant covered by bacteria. A total of 24 implants were used in this study, 12 test and 12 control. Surface characterization of the machined portion of the neck of the implant was performed on an additional 10 implants (5 test and 5 control). On test implants the implant surface covered by bacteria was significantly lower compared with that of control implants (P = .0001). The surface roughness was similar in both groups. TiN surfaces showed a significant reduction of the presence of bacteria, and this fact could probably be important in the decrease of the inflammation of the peri-implant soft tissues.

Adult↗